Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13015 and RR42_RS13020 overlap by 1 nucleotides RR42_RS13020 and RR42_RS13025 are separated by 98 nucleotides RR42_RS13025 and RR42_RS13030 are separated by 137 nucleotides
RR42_RS13015: RR42_RS13015 - fatty acid hydroxylase, at 2,801,413 to 2,802,387
_RS13015
RR42_RS13020: RR42_RS13020 - peptidase A8, at 2,802,387 to 2,803,268
_RS13020
RR42_RS13025: RR42_RS13025 - hypothetical protein, at 2,803,367 to 2,804,353
_RS13025
RR42_RS13030: RR42_RS13030 - ATP-dependent helicase, at 2,804,491 to 2,808,594
_RS13030
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2803.043 kb on + strand, within RR42_RS13020 at 2803.044 kb on - strand, within RR42_RS13020 at 2803.044 kb on - strand, within RR42_RS13020 at 2803.112 kb on - strand, within RR42_RS13020 at 2803.112 kb on - strand, within RR42_RS13020 at 2803.319 kb on + strand at 2803.319 kb on + strand at 2803.319 kb on + strand at 2803.319 kb on + strand at 2803.319 kb on + strand at 2803.320 kb on - strand at 2803.320 kb on - strand at 2803.725 kb on + strand, within RR42_RS13025 at 2803.755 kb on + strand, within RR42_RS13025 at 2803.756 kb on - strand, within RR42_RS13025 at 2803.906 kb on + strand, within RR42_RS13025 at 2803.906 kb on + strand, within RR42_RS13025 at 2803.972 kb on - strand, within RR42_RS13025 at 2803.972 kb on - strand, within RR42_RS13025 at 2803.972 kb on - strand, within RR42_RS13025 at 2804.044 kb on - strand, within RR42_RS13025 at 2804.354 kb on - strand at 2804.374 kb on + strand at 2804.375 kb on - strand at 2804.387 kb on + strand at 2804.388 kb on - strand at 2804.430 kb on - strand at 2804.430 kb on - strand at 2804.430 kb on - strand at 2804.430 kb on - strand at 2804.766 kb on - strand at 2804.793 kb on - strand at 2804.793 kb on - strand at 2804.906 kb on + strand, within RR42_RS13030 at 2804.906 kb on + strand, within RR42_RS13030 at 2804.906 kb on + strand, within RR42_RS13030 at 2804.907 kb on - strand, within RR42_RS13030 at 2805.095 kb on + strand, within RR42_RS13030 at 2805.095 kb on + strand, within RR42_RS13030 at 2805.095 kb on + strand, within RR42_RS13030 at 2805.095 kb on + strand, within RR42_RS13030 at 2805.096 kb on - strand, within RR42_RS13030 at 2805.096 kb on - strand, within RR42_RS13030 at 2805.096 kb on - strand, within RR42_RS13030 at 2805.181 kb on + strand, within RR42_RS13030 at 2805.275 kb on + strand, within RR42_RS13030 at 2805.275 kb on + strand, within RR42_RS13030 at 2805.275 kb on + strand, within RR42_RS13030 at 2805.276 kb on - strand, within RR42_RS13030 at 2805.276 kb on - strand, within RR42_RS13030 at 2805.351 kb on - strand, within RR42_RS13030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,803,043 + RR42_RS13020 0.74 -0.4 2,803,044 - RR42_RS13020 0.74 -2.6 2,803,044 - RR42_RS13020 0.74 +1.4 2,803,112 - RR42_RS13020 0.82 -0.3 2,803,112 - RR42_RS13020 0.82 +0.0 2,803,319 + -0.2 2,803,319 + +0.4 2,803,319 + +0.4 2,803,319 + +0.8 2,803,319 + +0.4 2,803,320 - +0.1 2,803,320 - -0.1 2,803,725 + RR42_RS13025 0.36 -1.1 2,803,755 + RR42_RS13025 0.39 -0.3 2,803,756 - RR42_RS13025 0.39 -1.0 2,803,906 + RR42_RS13025 0.55 +0.2 2,803,906 + RR42_RS13025 0.55 -2.3 2,803,972 - RR42_RS13025 0.61 -0.2 2,803,972 - RR42_RS13025 0.61 -3.6 2,803,972 - RR42_RS13025 0.61 -2.1 2,804,044 - RR42_RS13025 0.69 -0.6 2,804,354 - -0.7 2,804,374 + -2.0 2,804,375 - +1.0 2,804,387 + -0.0 2,804,388 - +0.0 2,804,430 - +0.2 2,804,430 - -0.0 2,804,430 - -0.2 2,804,430 - +1.4 2,804,766 - -0.3 2,804,793 - +1.0 2,804,793 - -0.4 2,804,906 + RR42_RS13030 0.10 +1.3 2,804,906 + RR42_RS13030 0.10 +1.4 2,804,906 + RR42_RS13030 0.10 +0.7 2,804,907 - RR42_RS13030 0.10 +0.3 2,805,095 + RR42_RS13030 0.15 +0.3 2,805,095 + RR42_RS13030 0.15 -0.0 2,805,095 + RR42_RS13030 0.15 +2.0 2,805,095 + RR42_RS13030 0.15 +0.7 2,805,096 - RR42_RS13030 0.15 +0.4 2,805,096 - RR42_RS13030 0.15 +0.3 2,805,096 - RR42_RS13030 0.15 +0.3 2,805,181 + RR42_RS13030 0.17 -0.3 2,805,275 + RR42_RS13030 0.19 +1.0 2,805,275 + RR42_RS13030 0.19 -1.2 2,805,275 + RR42_RS13030 0.19 +1.0 2,805,276 - RR42_RS13030 0.19 +0.3 2,805,276 - RR42_RS13030 0.19 +2.6 2,805,351 - RR42_RS13030 0.21 +0.4
Or see this region's nucleotide sequence