Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12895 and RR42_RS12900 are separated by 3 nucleotides RR42_RS12900 and RR42_RS12905 are separated by 3 nucleotides RR42_RS12905 and RR42_RS12910 are separated by 19 nucleotides RR42_RS12910 and RR42_RS12915 are separated by 188 nucleotides RR42_RS12915 and RR42_RS12920 are separated by 4 nucleotides
RR42_RS12895: RR42_RS12895 - cytochrome oxidase subunit III, at 2,771,822 to 2,772,748
_RS12895
RR42_RS12900: RR42_RS12900 - hypothetical protein, at 2,772,752 to 2,772,979
_RS12900
RR42_RS12905: RR42_RS12905 - peptidase S41, at 2,772,983 to 2,773,660
_RS12905
RR42_RS12910: RR42_RS12910 - cbb3-type cytochrome c oxidase subunit I, at 2,773,680 to 2,775,128
_RS12910
RR42_RS12915: RR42_RS12915 - cytochrome oxidase maturation protein Cbb3, at 2,775,317 to 2,775,463
_RS12915
RR42_RS12920: RR42_RS12920 - carbonate dehydratase, at 2,775,468 to 2,778,038
_RS12920
Position (kb)
2773
2774
2775
2776 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2772.714 kb on + strand at 2772.714 kb on + strand at 2772.714 kb on + strand at 2772.715 kb on - strand at 2772.715 kb on - strand at 2772.715 kb on - strand at 2772.985 kb on - strand at 2773.386 kb on + strand, within RR42_RS12905 at 2773.395 kb on + strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.465 kb on - strand, within RR42_RS12905 at 2773.468 kb on + strand, within RR42_RS12905 at 2773.468 kb on + strand, within RR42_RS12905 at 2773.759 kb on + strand at 2773.759 kb on + strand at 2773.760 kb on - strand at 2773.760 kb on - strand at 2773.826 kb on - strand, within RR42_RS12910 at 2773.855 kb on + strand, within RR42_RS12910 at 2773.855 kb on + strand, within RR42_RS12910 at 2773.934 kb on - strand, within RR42_RS12910 at 2773.934 kb on - strand, within RR42_RS12910 at 2773.991 kb on - strand, within RR42_RS12910 at 2774.027 kb on - strand, within RR42_RS12910 at 2774.027 kb on - strand, within RR42_RS12910 at 2774.167 kb on + strand, within RR42_RS12910 at 2774.363 kb on + strand, within RR42_RS12910 at 2774.364 kb on - strand, within RR42_RS12910 at 2774.364 kb on - strand, within RR42_RS12910 at 2774.364 kb on - strand, within RR42_RS12910 at 2774.365 kb on + strand, within RR42_RS12910 at 2774.365 kb on + strand, within RR42_RS12910 at 2774.366 kb on - strand, within RR42_RS12910 at 2774.413 kb on + strand, within RR42_RS12910 at 2774.414 kb on - strand, within RR42_RS12910 at 2774.447 kb on - strand, within RR42_RS12910 at 2774.449 kb on + strand, within RR42_RS12910 at 2774.450 kb on - strand, within RR42_RS12910 at 2774.450 kb on - strand, within RR42_RS12910 at 2774.618 kb on - strand, within RR42_RS12910 at 2774.618 kb on - strand, within RR42_RS12910 at 2774.644 kb on + strand, within RR42_RS12910 at 2774.645 kb on - strand, within RR42_RS12910 at 2774.648 kb on + strand, within RR42_RS12910 at 2774.649 kb on - strand, within RR42_RS12910 at 2774.695 kb on + strand, within RR42_RS12910 at 2774.749 kb on + strand, within RR42_RS12910 at 2774.881 kb on + strand, within RR42_RS12910 at 2775.024 kb on + strand at 2775.086 kb on - strand at 2775.109 kb on + strand at 2775.110 kb on - strand at 2775.451 kb on - strand at 2775.603 kb on - strand at 2775.845 kb on - strand, within RR42_RS12920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,772,714 + -0.3 2,772,714 + +0.3 2,772,714 + -1.3 2,772,715 - -1.4 2,772,715 - -0.7 2,772,715 - -0.8 2,772,985 - -0.4 2,773,386 + RR42_RS12905 0.59 -0.3 2,773,395 + RR42_RS12905 0.61 +1.0 2,773,465 - RR42_RS12905 0.71 +2.5 2,773,465 - RR42_RS12905 0.71 -1.2 2,773,465 - RR42_RS12905 0.71 +0.3 2,773,465 - RR42_RS12905 0.71 -1.0 2,773,465 - RR42_RS12905 0.71 -1.1 2,773,465 - RR42_RS12905 0.71 +1.8 2,773,468 + RR42_RS12905 0.72 -0.3 2,773,468 + RR42_RS12905 0.72 -0.1 2,773,759 + -0.5 2,773,759 + +0.0 2,773,760 - -0.5 2,773,760 - +0.1 2,773,826 - RR42_RS12910 0.10 +0.6 2,773,855 + RR42_RS12910 0.12 -0.0 2,773,855 + RR42_RS12910 0.12 -0.6 2,773,934 - RR42_RS12910 0.18 -0.9 2,773,934 - RR42_RS12910 0.18 +1.0 2,773,991 - RR42_RS12910 0.21 -0.1 2,774,027 - RR42_RS12910 0.24 -1.4 2,774,027 - RR42_RS12910 0.24 -1.3 2,774,167 + RR42_RS12910 0.34 +0.7 2,774,363 + RR42_RS12910 0.47 -0.2 2,774,364 - RR42_RS12910 0.47 -0.5 2,774,364 - RR42_RS12910 0.47 -0.7 2,774,364 - RR42_RS12910 0.47 -0.2 2,774,365 + RR42_RS12910 0.47 -0.5 2,774,365 + RR42_RS12910 0.47 -0.1 2,774,366 - RR42_RS12910 0.47 -0.3 2,774,413 + RR42_RS12910 0.51 -0.1 2,774,414 - RR42_RS12910 0.51 -0.3 2,774,447 - RR42_RS12910 0.53 -0.4 2,774,449 + RR42_RS12910 0.53 -1.6 2,774,450 - RR42_RS12910 0.53 +0.2 2,774,450 - RR42_RS12910 0.53 -0.4 2,774,618 - RR42_RS12910 0.65 -1.0 2,774,618 - RR42_RS12910 0.65 +0.6 2,774,644 + RR42_RS12910 0.67 -0.4 2,774,645 - RR42_RS12910 0.67 -0.6 2,774,648 + RR42_RS12910 0.67 +0.7 2,774,649 - RR42_RS12910 0.67 -0.9 2,774,695 + RR42_RS12910 0.70 -0.0 2,774,749 + RR42_RS12910 0.74 -0.7 2,774,881 + RR42_RS12910 0.83 -1.8 2,775,024 + +0.3 2,775,086 - -1.1 2,775,109 + -1.9 2,775,110 - -0.8 2,775,451 - -1.3 2,775,603 - +0.5 2,775,845 - RR42_RS12920 0.15 -0.6
Or see this region's nucleotide sequence