Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12060 and RR42_RS12065 are separated by 195 nucleotides RR42_RS12065 and RR42_RS12070 are separated by 47 nucleotides RR42_RS12070 and RR42_RS12075 overlap by 4 nucleotides RR42_RS12075 and RR42_RS12080 are separated by 81 nucleotides
RR42_RS12060: RR42_RS12060 - O-sialoglycoprotein endopeptidase, at 2,585,287 to 2,586,054
_RS12060
RR42_RS12065: RR42_RS12065 - acyl-CoA-binding protein, at 2,586,250 to 2,586,519
_RS12065
RR42_RS12070: RR42_RS12070 - MFS transporter, at 2,586,567 to 2,588,036
_RS12070
RR42_RS12075: RR42_RS12075 - hypothetical protein, at 2,588,033 to 2,588,503
_RS12075
RR42_RS12080: RR42_RS12080 - lipase, at 2,588,585 to 2,589,301
_RS12080
Position (kb)
2586
2587
2588
2589 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2586.176 kb on + strand at 2586.176 kb on + strand at 2586.177 kb on - strand at 2586.177 kb on - strand at 2586.340 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.475 kb on + strand, within RR42_RS12065 at 2586.476 kb on - strand, within RR42_RS12065 at 2586.817 kb on + strand, within RR42_RS12070 at 2586.910 kb on + strand, within RR42_RS12070 at 2587.085 kb on + strand, within RR42_RS12070 at 2587.085 kb on + strand, within RR42_RS12070 at 2587.085 kb on + strand, within RR42_RS12070 at 2587.088 kb on - strand, within RR42_RS12070 at 2587.088 kb on - strand, within RR42_RS12070 at 2587.108 kb on + strand, within RR42_RS12070 at 2587.108 kb on + strand, within RR42_RS12070 at 2587.124 kb on - strand, within RR42_RS12070 at 2587.195 kb on + strand, within RR42_RS12070 at 2587.607 kb on + strand, within RR42_RS12070 at 2587.675 kb on + strand, within RR42_RS12070 at 2587.675 kb on + strand, within RR42_RS12070 at 2587.675 kb on + strand, within RR42_RS12070 at 2587.676 kb on - strand, within RR42_RS12070 at 2587.676 kb on - strand, within RR42_RS12070 at 2587.676 kb on - strand, within RR42_RS12070 at 2587.897 kb on - strand at 2588.169 kb on + strand, within RR42_RS12075 at 2588.461 kb on + strand at 2588.520 kb on + strand at 2588.520 kb on + strand at 2588.521 kb on - strand at 2588.521 kb on - strand at 2588.534 kb on - strand at 2588.536 kb on + strand at 2588.536 kb on + strand at 2588.536 kb on + strand at 2588.536 kb on + strand at 2588.536 kb on + strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.537 kb on - strand at 2588.649 kb on + strand at 2588.649 kb on + strand at 2588.649 kb on + strand at 2588.649 kb on + strand at 2588.649 kb on + strand at 2588.650 kb on - strand at 2588.657 kb on + strand, within RR42_RS12080 at 2588.658 kb on - strand, within RR42_RS12080 at 2588.787 kb on + strand, within RR42_RS12080 at 2588.787 kb on + strand, within RR42_RS12080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,586,176 + -0.6 2,586,176 + -0.4 2,586,177 - -0.6 2,586,177 - -1.1 2,586,340 + RR42_RS12065 0.33 -0.7 2,586,415 + RR42_RS12065 0.61 +0.0 2,586,415 + RR42_RS12065 0.61 -2.3 2,586,475 + RR42_RS12065 0.83 -0.4 2,586,476 - RR42_RS12065 0.84 -0.1 2,586,817 + RR42_RS12070 0.17 +0.5 2,586,910 + RR42_RS12070 0.23 -0.1 2,587,085 + RR42_RS12070 0.35 +1.0 2,587,085 + RR42_RS12070 0.35 +1.0 2,587,085 + RR42_RS12070 0.35 +0.1 2,587,088 - RR42_RS12070 0.35 +1.2 2,587,088 - RR42_RS12070 0.35 -1.4 2,587,108 + RR42_RS12070 0.37 +0.9 2,587,108 + RR42_RS12070 0.37 -0.7 2,587,124 - RR42_RS12070 0.38 +0.4 2,587,195 + RR42_RS12070 0.43 +0.7 2,587,607 + RR42_RS12070 0.71 +0.0 2,587,675 + RR42_RS12070 0.75 -0.1 2,587,675 + RR42_RS12070 0.75 -0.7 2,587,675 + RR42_RS12070 0.75 +1.2 2,587,676 - RR42_RS12070 0.75 -0.6 2,587,676 - RR42_RS12070 0.75 +0.2 2,587,676 - RR42_RS12070 0.75 +1.0 2,587,897 - +0.6 2,588,169 + RR42_RS12075 0.29 -0.0 2,588,461 + +0.9 2,588,520 + -0.6 2,588,520 + -2.8 2,588,521 - -0.0 2,588,521 - +0.4 2,588,534 - +0.9 2,588,536 + +0.3 2,588,536 + -1.2 2,588,536 + -1.8 2,588,536 + +0.3 2,588,536 + -1.1 2,588,537 - +0.7 2,588,537 - -0.5 2,588,537 - -0.4 2,588,537 - -0.1 2,588,537 - +0.3 2,588,537 - -0.4 2,588,537 - -0.3 2,588,649 + +0.0 2,588,649 + -0.3 2,588,649 + +0.1 2,588,649 + -0.9 2,588,649 + -0.1 2,588,650 - -1.8 2,588,657 + RR42_RS12080 0.10 -0.5 2,588,658 - RR42_RS12080 0.10 +0.9 2,588,787 + RR42_RS12080 0.28 +0.5 2,588,787 + RR42_RS12080 0.28 -0.1
Or see this region's nucleotide sequence