Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11845 and RR42_RS11850 are separated by 11 nucleotides RR42_RS11850 and RR42_RS11855 are separated by 37 nucleotides RR42_RS11855 and RR42_RS11860 are separated by 53 nucleotides RR42_RS11860 and RR42_RS11865 are separated by 120 nucleotides
RR42_RS11845: RR42_RS11845 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 2,533,165 to 2,534,343
_RS11845
RR42_RS11850: RR42_RS11850 - phosphatidate cytidylyltransferase, at 2,534,355 to 2,535,176
_RS11850
RR42_RS11855: RR42_RS11855 - UDP diphosphate synthase, at 2,535,214 to 2,535,987
_RS11855
RR42_RS11860: RR42_RS11860 - ribosome recycling factor, at 2,536,041 to 2,536,601
_RS11860
RR42_RS11865: RR42_RS11865 - uridylate kinase, at 2,536,722 to 2,537,432
_RS11865
Position (kb)
2535
2536 Strain fitness (log2 ratio)
-1
0
1 at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2536.622 kb on - strand at 2536.723 kb on + strand at 2536.724 kb on - strand at 2536.726 kb on - strand at 2536.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,534,363 - -0.3 2,534,363 - +1.3 2,534,363 - +0.2 2,534,363 - -1.0 2,536,622 - +1.2 2,536,723 + -0.2 2,536,724 - +0.1 2,536,726 - +0.8 2,536,726 - -0.7
Or see this region's nucleotide sequence