Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11760 and RR42_RS11765 are separated by 29 nucleotides RR42_RS11765 and RR42_RS11770 overlap by 8 nucleotides RR42_RS11770 and RR42_RS11775 are separated by 238 nucleotides RR42_RS11775 and RR42_RS11780 are separated by 74 nucleotides
RR42_RS11760: RR42_RS11760 - hypothetical protein, at 2,516,047 to 2,516,628
_RS11760
RR42_RS11765: RR42_RS11765 - protein RnfH, at 2,516,658 to 2,517,005
_RS11765
RR42_RS11770: RR42_RS11770 - cyclase, at 2,516,998 to 2,517,435
_RS11770
RR42_RS11775: RR42_RS11775 - single-stranded DNA-binding protein, at 2,517,674 to 2,518,126
_RS11775
RR42_RS11780: RR42_RS11780 - stomatin 2, at 2,518,201 to 2,519,133
_RS11780
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2516.041 kb on + strand at 2516.042 kb on - strand at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.171 kb on - strand, within RR42_RS11760 at 2516.494 kb on + strand, within RR42_RS11760 at 2516.494 kb on + strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.495 kb on - strand, within RR42_RS11760 at 2516.761 kb on + strand, within RR42_RS11765 at 2516.953 kb on + strand, within RR42_RS11765 at 2516.953 kb on + strand, within RR42_RS11765 at 2517.011 kb on + strand at 2517.012 kb on - strand at 2517.114 kb on - strand, within RR42_RS11770 at 2517.383 kb on + strand, within RR42_RS11770 at 2517.383 kb on + strand, within RR42_RS11770 at 2517.421 kb on - strand at 2517.421 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.438 kb on - strand at 2517.643 kb on + strand at 2518.124 kb on + strand at 2518.125 kb on - strand at 2518.328 kb on + strand, within RR42_RS11780 at 2518.328 kb on + strand, within RR42_RS11780 at 2518.328 kb on + strand, within RR42_RS11780 at 2518.329 kb on - strand, within RR42_RS11780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,516,041 + -0.1 2,516,042 - +0.6 2,516,171 - RR42_RS11760 0.21 -0.5 2,516,171 - RR42_RS11760 0.21 -0.5 2,516,171 - RR42_RS11760 0.21 -0.2 2,516,171 - RR42_RS11760 0.21 -0.0 2,516,494 + RR42_RS11760 0.77 -0.5 2,516,494 + RR42_RS11760 0.77 +0.4 2,516,495 - RR42_RS11760 0.77 +0.7 2,516,495 - RR42_RS11760 0.77 -0.5 2,516,495 - RR42_RS11760 0.77 -0.2 2,516,761 + RR42_RS11765 0.30 -0.2 2,516,953 + RR42_RS11765 0.85 +0.4 2,516,953 + RR42_RS11765 0.85 -0.4 2,517,011 + +0.5 2,517,012 - -0.1 2,517,114 - RR42_RS11770 0.26 -3.2 2,517,383 + RR42_RS11770 0.88 -0.8 2,517,383 + RR42_RS11770 0.88 -0.2 2,517,421 - -3.5 2,517,421 - -2.4 2,517,438 - -0.8 2,517,438 - +0.8 2,517,438 - +0.0 2,517,438 - -0.3 2,517,438 - +0.7 2,517,643 + +0.1 2,518,124 + +0.4 2,518,125 - +0.2 2,518,328 + RR42_RS11780 0.14 -0.1 2,518,328 + RR42_RS11780 0.14 -0.4 2,518,328 + RR42_RS11780 0.14 -0.3 2,518,329 - RR42_RS11780 0.14 +0.5
Or see this region's nucleotide sequence