Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11610 and RR42_RS11615 are separated by 86 nucleotides RR42_RS11615 and RR42_RS11620 are separated by 110 nucleotides
RR42_RS11610: RR42_RS11610 - serine hydrolase, at 2,484,593 to 2,486,884
_RS11610
RR42_RS11615: RR42_RS11615 - GTP-binding protein, at 2,486,971 to 2,488,245
_RS11615
RR42_RS11620: RR42_RS11620 - polysaccharide deacetylase, at 2,488,356 to 2,489,111
_RS11620
Position (kb)
2486
2487
2488
2489 Strain fitness (log2 ratio)
-2
-1
0
1 at 2486.407 kb on + strand, within RR42_RS11610 at 2486.408 kb on - strand, within RR42_RS11610 at 2486.409 kb on + strand, within RR42_RS11610 at 2486.409 kb on + strand, within RR42_RS11610 at 2486.410 kb on - strand, within RR42_RS11610 at 2486.410 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.524 kb on + strand, within RR42_RS11610 at 2486.582 kb on + strand, within RR42_RS11610 at 2486.653 kb on + strand, within RR42_RS11610 at 2486.945 kb on + strand at 2486.996 kb on + strand at 2486.996 kb on + strand at 2486.997 kb on - strand at 2487.033 kb on + strand at 2487.190 kb on + strand, within RR42_RS11615 at 2487.190 kb on + strand, within RR42_RS11615 at 2487.190 kb on + strand, within RR42_RS11615 at 2487.255 kb on - strand, within RR42_RS11615 at 2487.272 kb on + strand, within RR42_RS11615 at 2487.273 kb on - strand, within RR42_RS11615 at 2487.374 kb on + strand, within RR42_RS11615 at 2487.488 kb on + strand, within RR42_RS11615 at 2487.853 kb on - strand, within RR42_RS11615 at 2487.927 kb on - strand, within RR42_RS11615 at 2488.005 kb on - strand, within RR42_RS11615 at 2488.101 kb on + strand, within RR42_RS11615 at 2488.109 kb on - strand, within RR42_RS11615 at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.292 kb on + strand at 2488.293 kb on - strand at 2488.510 kb on + strand, within RR42_RS11620 at 2488.729 kb on + strand, within RR42_RS11620 at 2488.760 kb on + strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2488.761 kb on - strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.011 kb on + strand, within RR42_RS11620 at 2489.012 kb on - strand, within RR42_RS11620 at 2489.092 kb on - strand at 2489.117 kb on - strand at 2489.245 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,486,407 + RR42_RS11610 0.79 +0.2 2,486,408 - RR42_RS11610 0.79 -0.5 2,486,409 + RR42_RS11610 0.79 -1.2 2,486,409 + RR42_RS11610 0.79 -0.4 2,486,410 - RR42_RS11610 0.79 -0.8 2,486,410 - RR42_RS11610 0.79 +1.0 2,486,466 - RR42_RS11610 0.82 +0.9 2,486,466 - RR42_RS11610 0.82 +0.1 2,486,466 - RR42_RS11610 0.82 -2.7 2,486,466 - RR42_RS11610 0.82 -0.0 2,486,524 + RR42_RS11610 0.84 -0.5 2,486,582 + RR42_RS11610 0.87 -0.5 2,486,653 + RR42_RS11610 0.90 -0.0 2,486,945 + +0.2 2,486,996 + -0.7 2,486,996 + -0.1 2,486,997 - +0.6 2,487,033 + +0.1 2,487,190 + RR42_RS11615 0.17 -1.3 2,487,190 + RR42_RS11615 0.17 +0.5 2,487,190 + RR42_RS11615 0.17 -0.1 2,487,255 - RR42_RS11615 0.22 +0.6 2,487,272 + RR42_RS11615 0.24 -0.2 2,487,273 - RR42_RS11615 0.24 +1.1 2,487,374 + RR42_RS11615 0.32 -0.6 2,487,488 + RR42_RS11615 0.41 +0.8 2,487,853 - RR42_RS11615 0.69 -0.6 2,487,927 - RR42_RS11615 0.75 +0.6 2,488,005 - RR42_RS11615 0.81 -0.8 2,488,101 + RR42_RS11615 0.89 +0.1 2,488,109 - RR42_RS11615 0.89 +0.1 2,488,292 + -0.0 2,488,292 + +0.2 2,488,292 + +0.2 2,488,293 - +0.2 2,488,510 + RR42_RS11620 0.20 +0.1 2,488,729 + RR42_RS11620 0.49 +0.0 2,488,760 + RR42_RS11620 0.53 -0.8 2,488,761 - RR42_RS11620 0.54 +0.4 2,488,761 - RR42_RS11620 0.54 -0.2 2,489,011 + RR42_RS11620 0.87 -0.7 2,489,011 + RR42_RS11620 0.87 +0.9 2,489,012 - RR42_RS11620 0.87 -0.8 2,489,092 - +1.1 2,489,117 - -0.2 2,489,245 - -0.5
Or see this region's nucleotide sequence