Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11600 and RR42_RS11605 are separated by 171 nucleotides RR42_RS11605 and RR42_RS11610 are separated by 149 nucleotides RR42_RS11610 and RR42_RS11615 are separated by 86 nucleotides
RR42_RS11600: RR42_RS11600 - flavin reductase, at 2,483,085 to 2,483,627
_RS11600
RR42_RS11605: RR42_RS11605 - OsmY domain-containing protein, at 2,483,799 to 2,484,443
_RS11605
RR42_RS11610: RR42_RS11610 - serine hydrolase, at 2,484,593 to 2,486,884
_RS11610
RR42_RS11615: RR42_RS11615 - GTP-binding protein, at 2,486,971 to 2,488,245
_RS11615
Position (kb)
2484
2485
2486
2487 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2483.656 kb on + strand at 2483.682 kb on - strand at 2483.682 kb on - strand at 2483.806 kb on - strand at 2483.936 kb on + strand, within RR42_RS11605 at 2483.936 kb on + strand, within RR42_RS11605 at 2484.313 kb on + strand, within RR42_RS11605 at 2484.314 kb on - strand, within RR42_RS11605 at 2484.343 kb on - strand, within RR42_RS11605 at 2484.432 kb on - strand at 2484.460 kb on - strand at 2484.460 kb on - strand at 2484.503 kb on + strand at 2484.503 kb on + strand at 2484.534 kb on + strand at 2484.534 kb on + strand at 2484.534 kb on + strand at 2484.534 kb on + strand at 2484.534 kb on + strand at 2484.597 kb on + strand at 2484.597 kb on + strand at 2484.602 kb on + strand at 2484.754 kb on - strand at 2484.922 kb on - strand, within RR42_RS11610 at 2484.922 kb on - strand, within RR42_RS11610 at 2485.033 kb on - strand, within RR42_RS11610 at 2485.034 kb on + strand, within RR42_RS11610 at 2485.034 kb on + strand, within RR42_RS11610 at 2485.051 kb on + strand at 2485.051 kb on + strand, within RR42_RS11610 at 2485.120 kb on - strand, within RR42_RS11610 at 2485.194 kb on - strand, within RR42_RS11610 at 2485.194 kb on - strand, within RR42_RS11610 at 2485.353 kb on + strand, within RR42_RS11610 at 2485.372 kb on - strand, within RR42_RS11610 at 2485.375 kb on + strand, within RR42_RS11610 at 2485.375 kb on + strand, within RR42_RS11610 at 2485.375 kb on + strand, within RR42_RS11610 at 2485.375 kb on + strand, within RR42_RS11610 at 2485.465 kb on - strand, within RR42_RS11610 at 2485.471 kb on + strand, within RR42_RS11610 at 2485.472 kb on - strand, within RR42_RS11610 at 2485.691 kb on + strand, within RR42_RS11610 at 2485.692 kb on - strand, within RR42_RS11610 at 2485.692 kb on - strand, within RR42_RS11610 at 2485.866 kb on + strand, within RR42_RS11610 at 2486.407 kb on + strand, within RR42_RS11610 at 2486.408 kb on - strand, within RR42_RS11610 at 2486.409 kb on + strand, within RR42_RS11610 at 2486.409 kb on + strand, within RR42_RS11610 at 2486.410 kb on - strand, within RR42_RS11610 at 2486.410 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.466 kb on - strand, within RR42_RS11610 at 2486.524 kb on + strand, within RR42_RS11610 at 2486.582 kb on + strand, within RR42_RS11610 at 2486.653 kb on + strand, within RR42_RS11610 at 2486.945 kb on + strand at 2486.996 kb on + strand at 2486.996 kb on + strand at 2486.997 kb on - strand at 2487.033 kb on + strand at 2487.190 kb on + strand, within RR42_RS11615 at 2487.190 kb on + strand, within RR42_RS11615 at 2487.190 kb on + strand, within RR42_RS11615 at 2487.255 kb on - strand, within RR42_RS11615 at 2487.272 kb on + strand, within RR42_RS11615 at 2487.273 kb on - strand, within RR42_RS11615 at 2487.374 kb on + strand, within RR42_RS11615 at 2487.488 kb on + strand, within RR42_RS11615 at 2487.853 kb on - strand, within RR42_RS11615
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,483,656 + +0.0 2,483,682 - -1.8 2,483,682 - -0.9 2,483,806 - -0.5 2,483,936 + RR42_RS11605 0.21 -0.8 2,483,936 + RR42_RS11605 0.21 +1.1 2,484,313 + RR42_RS11605 0.80 +1.0 2,484,314 - RR42_RS11605 0.80 +0.2 2,484,343 - RR42_RS11605 0.84 +0.3 2,484,432 - -0.4 2,484,460 - +0.3 2,484,460 - -1.4 2,484,503 + +1.2 2,484,503 + +0.4 2,484,534 + +0.7 2,484,534 + +0.2 2,484,534 + +0.0 2,484,534 + -0.2 2,484,534 + +1.0 2,484,597 + -0.1 2,484,597 + -0.2 2,484,602 + +0.7 2,484,754 - +1.1 2,484,922 - RR42_RS11610 0.14 -0.4 2,484,922 - RR42_RS11610 0.14 -0.2 2,485,033 - RR42_RS11610 0.19 -0.0 2,485,034 + RR42_RS11610 0.19 +0.1 2,485,034 + RR42_RS11610 0.19 +0.4 2,485,051 + -0.8 2,485,051 + RR42_RS11610 0.20 +0.5 2,485,120 - RR42_RS11610 0.23 -0.1 2,485,194 - RR42_RS11610 0.26 +0.5 2,485,194 - RR42_RS11610 0.26 -0.9 2,485,353 + RR42_RS11610 0.33 -2.4 2,485,372 - RR42_RS11610 0.34 +1.8 2,485,375 + RR42_RS11610 0.34 +0.1 2,485,375 + RR42_RS11610 0.34 -1.3 2,485,375 + RR42_RS11610 0.34 -0.5 2,485,375 + RR42_RS11610 0.34 -1.3 2,485,465 - RR42_RS11610 0.38 +0.3 2,485,471 + RR42_RS11610 0.38 +0.3 2,485,472 - RR42_RS11610 0.38 -0.5 2,485,691 + RR42_RS11610 0.48 -0.3 2,485,692 - RR42_RS11610 0.48 -0.2 2,485,692 - RR42_RS11610 0.48 +0.8 2,485,866 + RR42_RS11610 0.56 +0.2 2,486,407 + RR42_RS11610 0.79 +0.2 2,486,408 - RR42_RS11610 0.79 -0.5 2,486,409 + RR42_RS11610 0.79 -1.2 2,486,409 + RR42_RS11610 0.79 -0.4 2,486,410 - RR42_RS11610 0.79 -0.8 2,486,410 - RR42_RS11610 0.79 +1.0 2,486,466 - RR42_RS11610 0.82 +0.9 2,486,466 - RR42_RS11610 0.82 +0.1 2,486,466 - RR42_RS11610 0.82 -2.7 2,486,466 - RR42_RS11610 0.82 -0.0 2,486,524 + RR42_RS11610 0.84 -0.5 2,486,582 + RR42_RS11610 0.87 -0.5 2,486,653 + RR42_RS11610 0.90 -0.0 2,486,945 + +0.2 2,486,996 + -0.7 2,486,996 + -0.1 2,486,997 - +0.6 2,487,033 + +0.1 2,487,190 + RR42_RS11615 0.17 -1.3 2,487,190 + RR42_RS11615 0.17 +0.5 2,487,190 + RR42_RS11615 0.17 -0.1 2,487,255 - RR42_RS11615 0.22 +0.6 2,487,272 + RR42_RS11615 0.24 -0.2 2,487,273 - RR42_RS11615 0.24 +1.1 2,487,374 + RR42_RS11615 0.32 -0.6 2,487,488 + RR42_RS11615 0.41 +0.8 2,487,853 - RR42_RS11615 0.69 -0.6
Or see this region's nucleotide sequence