Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10635 and RR42_RS10640 are separated by 769 nucleotides RR42_RS10640 and RR42_RS10645 are separated by 154 nucleotides RR42_RS10645 and RR42_RS10650 are separated by 177 nucleotides
RR42_RS10635: RR42_RS10635 - hypothetical protein, at 2,271,679 to 2,272,431
_RS10635
RR42_RS10640: RR42_RS10640 - alanine dehydrogenase, at 2,273,201 to 2,274,316
_RS10640
RR42_RS10645: RR42_RS10645 - hypothetical protein, at 2,274,471 to 2,274,818
_RS10645
RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762
_RS10650
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-2
-1
0
1 at 2272.556 kb on - strand at 2272.580 kb on - strand at 2272.591 kb on + strand at 2272.652 kb on - strand at 2272.751 kb on - strand at 2272.755 kb on - strand at 2272.777 kb on + strand at 2272.979 kb on - strand at 2273.119 kb on + strand at 2273.119 kb on + strand at 2273.120 kb on - strand at 2273.120 kb on - strand at 2273.130 kb on - strand at 2273.230 kb on - strand at 2273.230 kb on - strand at 2273.230 kb on - strand at 2273.417 kb on - strand, within RR42_RS10640 at 2273.430 kb on + strand, within RR42_RS10640 at 2273.431 kb on - strand, within RR42_RS10640 at 2273.454 kb on + strand, within RR42_RS10640 at 2273.454 kb on + strand, within RR42_RS10640 at 2273.454 kb on + strand, within RR42_RS10640 at 2273.454 kb on + strand, within RR42_RS10640 at 2273.454 kb on + strand, within RR42_RS10640 at 2273.455 kb on - strand, within RR42_RS10640 at 2273.576 kb on - strand, within RR42_RS10640 at 2273.662 kb on - strand, within RR42_RS10640 at 2273.756 kb on - strand, within RR42_RS10640 at 2273.759 kb on + strand, within RR42_RS10640 at 2273.759 kb on + strand, within RR42_RS10640 at 2273.759 kb on + strand, within RR42_RS10640 at 2273.760 kb on - strand, within RR42_RS10640 at 2273.760 kb on - strand, within RR42_RS10640 at 2273.760 kb on - strand, within RR42_RS10640 at 2273.968 kb on - strand, within RR42_RS10640 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.043 kb on - strand, within RR42_RS10640 at 2274.125 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.147 kb on - strand, within RR42_RS10640 at 2274.169 kb on - strand, within RR42_RS10640 at 2274.251 kb on - strand at 2274.472 kb on + strand at 2274.472 kb on + strand at 2274.473 kb on - strand at 2274.549 kb on + strand, within RR42_RS10645 at 2274.549 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.656 kb on + strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.894 kb on - strand at 2274.894 kb on - strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.896 kb on - strand at 2274.959 kb on - strand at 2274.966 kb on + strand at 2274.967 kb on - strand at 2274.967 kb on - strand at 2275.175 kb on + strand, within RR42_RS10650 at 2275.239 kb on + strand, within RR42_RS10650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,272,556 - -0.3 2,272,580 - +0.7 2,272,591 + -0.7 2,272,652 - +1.1 2,272,751 - -0.2 2,272,755 - -1.3 2,272,777 + +0.4 2,272,979 - +1.1 2,273,119 + -1.3 2,273,119 + +0.9 2,273,120 - +0.1 2,273,120 - -0.1 2,273,130 - -1.0 2,273,230 - -0.6 2,273,230 - +0.7 2,273,230 - -0.1 2,273,417 - RR42_RS10640 0.19 +0.1 2,273,430 + RR42_RS10640 0.21 +0.1 2,273,431 - RR42_RS10640 0.21 -1.3 2,273,454 + RR42_RS10640 0.23 -1.2 2,273,454 + RR42_RS10640 0.23 -1.0 2,273,454 + RR42_RS10640 0.23 -0.5 2,273,454 + RR42_RS10640 0.23 +0.2 2,273,454 + RR42_RS10640 0.23 +0.6 2,273,455 - RR42_RS10640 0.23 -1.0 2,273,576 - RR42_RS10640 0.34 +0.1 2,273,662 - RR42_RS10640 0.41 +0.4 2,273,756 - RR42_RS10640 0.50 +0.3 2,273,759 + RR42_RS10640 0.50 -1.0 2,273,759 + RR42_RS10640 0.50 -0.2 2,273,759 + RR42_RS10640 0.50 -1.4 2,273,760 - RR42_RS10640 0.50 -1.0 2,273,760 - RR42_RS10640 0.50 +0.5 2,273,760 - RR42_RS10640 0.50 -0.1 2,273,968 - RR42_RS10640 0.69 -0.0 2,274,037 - RR42_RS10640 0.75 +0.4 2,274,037 - RR42_RS10640 0.75 -1.0 2,274,043 - RR42_RS10640 0.75 +0.1 2,274,125 + RR42_RS10640 0.83 +0.2 2,274,146 + RR42_RS10640 0.85 +0.4 2,274,146 + RR42_RS10640 0.85 -0.7 2,274,147 - RR42_RS10640 0.85 -0.3 2,274,169 - RR42_RS10640 0.87 +0.2 2,274,251 - -0.9 2,274,472 + -1.3 2,274,472 + -0.1 2,274,473 - +0.1 2,274,549 + RR42_RS10645 0.22 -0.5 2,274,549 + RR42_RS10645 0.22 -0.5 2,274,654 + RR42_RS10645 0.53 +0.2 2,274,654 + RR42_RS10645 0.53 -0.6 2,274,655 - RR42_RS10645 0.53 +0.6 2,274,655 - RR42_RS10645 0.53 +0.7 2,274,656 + RR42_RS10645 0.53 +0.0 2,274,657 - RR42_RS10645 0.53 -0.6 2,274,657 - RR42_RS10645 0.53 -2.4 2,274,751 - RR42_RS10645 0.80 +0.3 2,274,751 - RR42_RS10645 0.80 -0.5 2,274,767 - RR42_RS10645 0.85 -0.2 2,274,767 - RR42_RS10645 0.85 +1.1 2,274,894 - -0.0 2,274,894 - +1.4 2,274,895 + -0.5 2,274,895 + -0.9 2,274,895 + +0.2 2,274,895 + +0.4 2,274,895 + +0.1 2,274,895 + +0.0 2,274,896 - -0.1 2,274,959 - -0.0 2,274,966 + -1.7 2,274,967 - +0.5 2,274,967 - -2.0 2,275,175 + RR42_RS10650 0.10 -0.4 2,275,239 + RR42_RS10650 0.14 +0.1
Or see this region's nucleotide sequence