Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10265 and RR42_RS10270 are separated by 52 nucleotides RR42_RS10270 and RR42_RS10275 are separated by 138 nucleotides RR42_RS10275 and RR42_RS10280 are separated by 3 nucleotides
RR42_RS10265: RR42_RS10265 - 3-hydroxyacyl-CoA dehydrogenase, at 2,197,332 to 2,199,410
_RS10265
RR42_RS10270: RR42_RS10270 - hypothetical protein, at 2,199,463 to 2,200,236
_RS10270
RR42_RS10275: RR42_RS10275 - LuxR family transcriptional regulator, at 2,200,375 to 2,201,007
_RS10275
RR42_RS10280: RR42_RS10280 - hypothetical protein, at 2,201,011 to 2,202,153
_RS10280
Position (kb)
2199
2200
2201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2198.523 kb on + strand, within RR42_RS10265 at 2198.802 kb on - strand, within RR42_RS10265 at 2198.815 kb on - strand, within RR42_RS10265 at 2198.815 kb on - strand, within RR42_RS10265 at 2199.077 kb on - strand, within RR42_RS10265 at 2199.138 kb on + strand, within RR42_RS10265 at 2199.138 kb on + strand, within RR42_RS10265 at 2199.149 kb on + strand, within RR42_RS10265 at 2199.150 kb on - strand, within RR42_RS10265 at 2199.150 kb on - strand, within RR42_RS10265 at 2199.168 kb on + strand, within RR42_RS10265 at 2199.554 kb on - strand, within RR42_RS10270 at 2199.554 kb on - strand, within RR42_RS10270 at 2199.627 kb on + strand, within RR42_RS10270 at 2199.627 kb on + strand, within RR42_RS10270 at 2199.627 kb on + strand, within RR42_RS10270 at 2199.627 kb on + strand, within RR42_RS10270 at 2199.627 kb on + strand, within RR42_RS10270 at 2199.794 kb on - strand, within RR42_RS10270 at 2200.142 kb on + strand, within RR42_RS10270 at 2200.353 kb on - strand at 2200.461 kb on - strand, within RR42_RS10275 at 2200.461 kb on - strand, within RR42_RS10275 at 2200.525 kb on + strand, within RR42_RS10275 at 2200.548 kb on - strand, within RR42_RS10275 at 2200.548 kb on - strand, within RR42_RS10275 at 2200.634 kb on - strand, within RR42_RS10275 at 2200.707 kb on - strand, within RR42_RS10275 at 2200.707 kb on - strand, within RR42_RS10275 at 2200.707 kb on - strand, within RR42_RS10275 at 2200.742 kb on + strand, within RR42_RS10275 at 2201.012 kb on + strand at 2201.231 kb on - strand, within RR42_RS10280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,198,523 + RR42_RS10265 0.57 +0.1 2,198,802 - RR42_RS10265 0.71 -0.1 2,198,815 - RR42_RS10265 0.71 -0.6 2,198,815 - RR42_RS10265 0.71 -0.0 2,199,077 - RR42_RS10265 0.84 +0.9 2,199,138 + RR42_RS10265 0.87 -0.1 2,199,138 + RR42_RS10265 0.87 -0.1 2,199,149 + RR42_RS10265 0.87 -0.6 2,199,150 - RR42_RS10265 0.87 -1.3 2,199,150 - RR42_RS10265 0.87 -0.7 2,199,168 + RR42_RS10265 0.88 -1.8 2,199,554 - RR42_RS10270 0.12 +2.4 2,199,554 - RR42_RS10270 0.12 +1.5 2,199,627 + RR42_RS10270 0.21 -1.6 2,199,627 + RR42_RS10270 0.21 +0.6 2,199,627 + RR42_RS10270 0.21 +0.3 2,199,627 + RR42_RS10270 0.21 +0.1 2,199,627 + RR42_RS10270 0.21 +0.4 2,199,794 - RR42_RS10270 0.43 -0.0 2,200,142 + RR42_RS10270 0.88 +0.5 2,200,353 - +1.1 2,200,461 - RR42_RS10275 0.14 -0.1 2,200,461 - RR42_RS10275 0.14 -1.1 2,200,525 + RR42_RS10275 0.24 -1.3 2,200,548 - RR42_RS10275 0.27 -0.3 2,200,548 - RR42_RS10275 0.27 +0.9 2,200,634 - RR42_RS10275 0.41 +1.6 2,200,707 - RR42_RS10275 0.52 +0.0 2,200,707 - RR42_RS10275 0.52 +1.1 2,200,707 - RR42_RS10275 0.52 +0.0 2,200,742 + RR42_RS10275 0.58 +0.7 2,201,012 + +0.1 2,201,231 - RR42_RS10280 0.19 +1.1
Or see this region's nucleotide sequence