Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09295 and RR42_RS09300 are separated by 56 nucleotides RR42_RS09300 and RR42_RS09305 are separated by 153 nucleotides
RR42_RS09295: RR42_RS09295 - phospholipase, at 1,965,842 to 1,966,546
_RS09295
RR42_RS09300: RR42_RS09300 - aromatic ring-opening dioxygenase LigB, at 1,966,603 to 1,967,433
_RS09300
RR42_RS09305: RR42_RS09305 - LysR family transcriptional regulator, at 1,967,587 to 1,968,483
_RS09305
Position (kb)
1966
1967
1968 Strain fitness (log2 ratio)
-1
0
1 at 1965.787 kb on - strand at 1965.787 kb on - strand at 1965.843 kb on + strand at 1966.652 kb on + strand at 1966.652 kb on + strand at 1966.652 kb on + strand at 1966.652 kb on + strand at 1966.652 kb on + strand at 1966.652 kb on + strand at 1966.653 kb on - strand at 1966.653 kb on - strand at 1966.653 kb on - strand at 1966.677 kb on - strand at 1966.677 kb on - strand at 1966.803 kb on - strand, within RR42_RS09300 at 1967.306 kb on - strand, within RR42_RS09300 at 1967.495 kb on - strand at 1967.495 kb on - strand at 1967.500 kb on - strand at 1967.500 kb on - strand at 1967.541 kb on + strand at 1967.543 kb on + strand at 1967.939 kb on - strand, within RR42_RS09305 at 1968.002 kb on - strand, within RR42_RS09305 at 1968.080 kb on + strand, within RR42_RS09305 at 1968.080 kb on + strand, within RR42_RS09305 at 1968.080 kb on + strand, within RR42_RS09305 at 1968.081 kb on - strand, within RR42_RS09305 at 1968.242 kb on - strand, within RR42_RS09305 at 1968.388 kb on + strand, within RR42_RS09305 at 1968.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,965,787 - -0.7 1,965,787 - +0.4 1,965,843 + +0.4 1,966,652 + +0.2 1,966,652 + -0.6 1,966,652 + -0.1 1,966,652 + +0.1 1,966,652 + +0.8 1,966,652 + +0.0 1,966,653 - +0.3 1,966,653 - -0.3 1,966,653 - +0.2 1,966,677 - -0.9 1,966,677 - +0.0 1,966,803 - RR42_RS09300 0.24 -0.9 1,967,306 - RR42_RS09300 0.85 -1.5 1,967,495 - -0.4 1,967,495 - +0.3 1,967,500 - +0.7 1,967,500 - -0.2 1,967,541 + +0.2 1,967,543 + +1.0 1,967,939 - RR42_RS09305 0.39 +0.5 1,968,002 - RR42_RS09305 0.46 -0.9 1,968,080 + RR42_RS09305 0.55 -0.4 1,968,080 + RR42_RS09305 0.55 +0.4 1,968,080 + RR42_RS09305 0.55 -0.5 1,968,081 - RR42_RS09305 0.55 -0.2 1,968,242 - RR42_RS09305 0.73 +0.3 1,968,388 + RR42_RS09305 0.89 +0.2 1,968,398 - -0.1
Or see this region's nucleotide sequence