Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08935 and RR42_RS08940 are separated by 91 nucleotides RR42_RS08940 and RR42_RS08945 are separated by 82 nucleotides RR42_RS08945 and RR42_RS08950 are separated by 97 nucleotides
RR42_RS08935: RR42_RS08935 - peptidylprolyl isomerase, at 1,879,953 to 1,880,741
_RS08935
RR42_RS08940: RR42_RS08940 - hypothetical protein, at 1,880,833 to 1,881,183
_RS08940
RR42_RS08945: RR42_RS08945 - hypothetical protein, at 1,881,266 to 1,881,628
_RS08945
RR42_RS08950: RR42_RS08950 - carbonic anhydrase, at 1,881,726 to 1,882,478
_RS08950
Position (kb)
1881
1882 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1880.587 kb on + strand, within RR42_RS08935 at 1880.587 kb on - strand, within RR42_RS08935 at 1880.588 kb on - strand, within RR42_RS08935 at 1880.643 kb on + strand, within RR42_RS08935 at 1880.643 kb on + strand, within RR42_RS08935 at 1880.643 kb on + strand, within RR42_RS08935 at 1880.644 kb on - strand, within RR42_RS08935 at 1880.644 kb on - strand, within RR42_RS08935 at 1880.644 kb on - strand, within RR42_RS08935 at 1880.665 kb on - strand at 1880.665 kb on - strand at 1880.734 kb on + strand at 1880.734 kb on + strand at 1880.734 kb on + strand at 1880.735 kb on - strand at 1880.735 kb on - strand at 1880.735 kb on - strand at 1880.739 kb on + strand at 1880.740 kb on - strand at 1880.802 kb on + strand at 1880.803 kb on - strand at 1881.158 kb on + strand at 1881.159 kb on - strand at 1881.159 kb on - strand at 1881.256 kb on + strand at 1881.256 kb on + strand at 1881.257 kb on - strand at 1881.257 kb on - strand at 1881.262 kb on + strand at 1881.263 kb on - strand at 1881.263 kb on - strand at 1881.263 kb on - strand at 1881.263 kb on - strand at 1881.263 kb on - strand at 1881.267 kb on + strand at 1881.267 kb on + strand at 1881.268 kb on - strand at 1881.268 kb on - strand at 1881.300 kb on + strand at 1881.300 kb on + strand at 1881.301 kb on - strand at 1881.394 kb on - strand, within RR42_RS08945 at 1881.676 kb on - strand at 1881.690 kb on + strand at 1881.691 kb on - strand at 1881.691 kb on - strand at 1881.691 kb on - strand at 1881.706 kb on - strand at 1881.763 kb on + strand at 1881.763 kb on + strand at 1881.818 kb on + strand, within RR42_RS08950 at 1881.818 kb on + strand, within RR42_RS08950 at 1881.818 kb on + strand, within RR42_RS08950 at 1881.818 kb on + strand, within RR42_RS08950 at 1881.819 kb on - strand, within RR42_RS08950 at 1881.819 kb on - strand, within RR42_RS08950 at 1881.819 kb on - strand, within RR42_RS08950 at 1882.029 kb on + strand, within RR42_RS08950 at 1882.029 kb on + strand, within RR42_RS08950 at 1882.030 kb on - strand, within RR42_RS08950 at 1882.030 kb on - strand, within RR42_RS08950 at 1882.094 kb on + strand, within RR42_RS08950 at 1882.107 kb on - strand, within RR42_RS08950 at 1882.234 kb on + strand, within RR42_RS08950 at 1882.235 kb on - strand, within RR42_RS08950 at 1882.272 kb on - strand, within RR42_RS08950 at 1882.272 kb on - strand, within RR42_RS08950 at 1882.349 kb on - strand, within RR42_RS08950 at 1882.349 kb on - strand, within RR42_RS08950 at 1882.421 kb on + strand at 1882.422 kb on - strand at 1882.422 kb on - strand at 1882.422 kb on - strand at 1882.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,880,587 + RR42_RS08935 0.80 -0.3 1,880,587 - RR42_RS08935 0.80 +0.3 1,880,588 - RR42_RS08935 0.80 -1.6 1,880,643 + RR42_RS08935 0.87 +0.5 1,880,643 + RR42_RS08935 0.87 +0.4 1,880,643 + RR42_RS08935 0.87 -1.7 1,880,644 - RR42_RS08935 0.88 +0.1 1,880,644 - RR42_RS08935 0.88 +0.5 1,880,644 - RR42_RS08935 0.88 +0.2 1,880,665 - -0.4 1,880,665 - +0.2 1,880,734 + +0.9 1,880,734 + -0.2 1,880,734 + +0.5 1,880,735 - +0.4 1,880,735 - -0.5 1,880,735 - +0.5 1,880,739 + -0.2 1,880,740 - +1.2 1,880,802 + -0.4 1,880,803 - -3.1 1,881,158 + +0.1 1,881,159 - +0.0 1,881,159 - -0.0 1,881,256 + +0.2 1,881,256 + +0.3 1,881,257 - +1.3 1,881,257 - +1.1 1,881,262 + -0.4 1,881,263 - -1.2 1,881,263 - +0.5 1,881,263 - +0.2 1,881,263 - -1.1 1,881,263 - -0.5 1,881,267 + -0.1 1,881,267 + +0.1 1,881,268 - +1.0 1,881,268 - -0.1 1,881,300 + +2.6 1,881,300 + +0.2 1,881,301 - +0.6 1,881,394 - RR42_RS08945 0.35 -1.0 1,881,676 - +0.4 1,881,690 + -0.2 1,881,691 - -0.5 1,881,691 - -0.8 1,881,691 - +0.2 1,881,706 - -0.5 1,881,763 + +0.4 1,881,763 + -0.7 1,881,818 + RR42_RS08950 0.12 +0.3 1,881,818 + RR42_RS08950 0.12 -0.6 1,881,818 + RR42_RS08950 0.12 -0.6 1,881,818 + RR42_RS08950 0.12 +1.4 1,881,819 - RR42_RS08950 0.12 +1.1 1,881,819 - RR42_RS08950 0.12 +0.1 1,881,819 - RR42_RS08950 0.12 +1.3 1,882,029 + RR42_RS08950 0.40 -0.2 1,882,029 + RR42_RS08950 0.40 -0.5 1,882,030 - RR42_RS08950 0.40 -0.6 1,882,030 - RR42_RS08950 0.40 -0.5 1,882,094 + RR42_RS08950 0.49 +0.0 1,882,107 - RR42_RS08950 0.51 +0.4 1,882,234 + RR42_RS08950 0.67 +0.3 1,882,235 - RR42_RS08950 0.68 +0.0 1,882,272 - RR42_RS08950 0.73 -0.7 1,882,272 - RR42_RS08950 0.73 -0.7 1,882,349 - RR42_RS08950 0.83 +0.1 1,882,349 - RR42_RS08950 0.83 +2.1 1,882,421 + +0.5 1,882,422 - +1.1 1,882,422 - +1.0 1,882,422 - -0.4 1,882,422 - -1.8
Or see this region's nucleotide sequence