Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08495 and RR42_RS08500 are separated by 107 nucleotides RR42_RS08500 and RR42_RS08505 are separated by 74 nucleotides RR42_RS08505 and RR42_RS08510 are separated by 317 nucleotides
RR42_RS08495: RR42_RS08495 - transcriptional regulator, at 1,789,025 to 1,789,273
_RS08495
RR42_RS08500: RR42_RS08500 - hypothetical protein, at 1,789,381 to 1,789,881
_RS08500
RR42_RS08505: RR42_RS08505 - DNA-binding protein, at 1,789,956 to 1,790,249
_RS08505
RR42_RS08510: RR42_RS08510 - hypothetical protein, at 1,790,567 to 1,790,989
_RS08510
Position (kb)
1789
1790
1791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1789.092 kb on + strand, within RR42_RS08495 at 1789.093 kb on - strand, within RR42_RS08495 at 1789.162 kb on - strand, within RR42_RS08495 at 1789.162 kb on - strand, within RR42_RS08495 at 1789.191 kb on + strand, within RR42_RS08495 at 1789.192 kb on - strand, within RR42_RS08495 at 1789.194 kb on - strand, within RR42_RS08495 at 1789.214 kb on + strand, within RR42_RS08495 at 1789.215 kb on - strand, within RR42_RS08495 at 1789.215 kb on - strand, within RR42_RS08495 at 1789.235 kb on - strand, within RR42_RS08495 at 1789.332 kb on + strand at 1789.333 kb on - strand at 1789.389 kb on + strand at 1789.389 kb on + strand at 1789.534 kb on + strand, within RR42_RS08500 at 1789.534 kb on + strand, within RR42_RS08500 at 1789.642 kb on + strand, within RR42_RS08500 at 1789.642 kb on + strand, within RR42_RS08500 at 1789.642 kb on + strand, within RR42_RS08500 at 1789.643 kb on - strand, within RR42_RS08500 at 1789.720 kb on + strand, within RR42_RS08500 at 1789.720 kb on + strand, within RR42_RS08500 at 1789.720 kb on + strand, within RR42_RS08500 at 1789.721 kb on - strand, within RR42_RS08500 at 1789.721 kb on - strand, within RR42_RS08500 at 1789.749 kb on + strand, within RR42_RS08500 at 1789.749 kb on + strand, within RR42_RS08500 at 1789.749 kb on + strand, within RR42_RS08500 at 1789.759 kb on + strand, within RR42_RS08500 at 1789.854 kb on + strand at 1789.855 kb on - strand at 1789.965 kb on + strand at 1789.965 kb on + strand at 1789.966 kb on - strand at 1789.966 kb on - strand at 1790.056 kb on + strand, within RR42_RS08505 at 1790.078 kb on - strand, within RR42_RS08505 at 1790.205 kb on + strand, within RR42_RS08505 at 1790.300 kb on + strand at 1790.301 kb on - strand at 1790.301 kb on - strand at 1790.349 kb on - strand at 1790.428 kb on + strand at 1790.428 kb on + strand at 1790.429 kb on - strand at 1790.441 kb on + strand at 1790.480 kb on + strand at 1790.500 kb on - strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.501 kb on + strand at 1790.502 kb on - strand at 1790.509 kb on + strand at 1790.509 kb on + strand at 1790.509 kb on + strand at 1790.509 kb on + strand at 1790.509 kb on + strand at 1790.509 kb on + strand at 1790.532 kb on + strand at 1790.927 kb on + strand, within RR42_RS08510 at 1790.956 kb on + strand at 1790.956 kb on + strand at 1791.021 kb on - strand at 1791.057 kb on - strand at 1791.130 kb on + strand at 1791.136 kb on - strand at 1791.239 kb on + strand at 1791.239 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,789,092 + RR42_RS08495 0.27 -1.3 1,789,093 - RR42_RS08495 0.27 -1.5 1,789,162 - RR42_RS08495 0.55 -0.2 1,789,162 - RR42_RS08495 0.55 -0.7 1,789,191 + RR42_RS08495 0.67 +1.0 1,789,192 - RR42_RS08495 0.67 -1.4 1,789,194 - RR42_RS08495 0.68 -1.2 1,789,214 + RR42_RS08495 0.76 +1.2 1,789,215 - RR42_RS08495 0.76 -0.3 1,789,215 - RR42_RS08495 0.76 +1.4 1,789,235 - RR42_RS08495 0.84 -1.9 1,789,332 + -0.6 1,789,333 - -1.1 1,789,389 + -0.5 1,789,389 + -1.8 1,789,534 + RR42_RS08500 0.31 +0.4 1,789,534 + RR42_RS08500 0.31 -1.2 1,789,642 + RR42_RS08500 0.52 -0.0 1,789,642 + RR42_RS08500 0.52 +2.1 1,789,642 + RR42_RS08500 0.52 +0.4 1,789,643 - RR42_RS08500 0.52 -2.8 1,789,720 + RR42_RS08500 0.68 -0.2 1,789,720 + RR42_RS08500 0.68 +0.9 1,789,720 + RR42_RS08500 0.68 -2.4 1,789,721 - RR42_RS08500 0.68 +0.0 1,789,721 - RR42_RS08500 0.68 -0.5 1,789,749 + RR42_RS08500 0.73 -1.5 1,789,749 + RR42_RS08500 0.73 -0.5 1,789,749 + RR42_RS08500 0.73 +0.1 1,789,759 + RR42_RS08500 0.75 +0.5 1,789,854 + -1.1 1,789,855 - -0.5 1,789,965 + +1.2 1,789,965 + +1.0 1,789,966 - -0.1 1,789,966 - -0.2 1,790,056 + RR42_RS08505 0.34 -1.7 1,790,078 - RR42_RS08505 0.41 -0.5 1,790,205 + RR42_RS08505 0.85 -1.5 1,790,300 + -1.5 1,790,301 - -1.4 1,790,301 - -0.5 1,790,349 - -0.5 1,790,428 + -1.2 1,790,428 + -0.8 1,790,429 - -0.3 1,790,441 + +0.0 1,790,480 + +0.2 1,790,500 - -0.3 1,790,501 + +0.7 1,790,501 + -0.5 1,790,501 + -1.4 1,790,501 + +0.8 1,790,501 + +0.5 1,790,501 + +1.2 1,790,501 + -0.4 1,790,501 + -0.1 1,790,501 + +1.1 1,790,502 - -1.8 1,790,509 + +0.3 1,790,509 + +1.3 1,790,509 + -0.2 1,790,509 + -2.0 1,790,509 + -0.7 1,790,509 + +1.2 1,790,532 + -0.1 1,790,927 + RR42_RS08510 0.85 +0.4 1,790,956 + +0.8 1,790,956 + +1.1 1,791,021 - +0.3 1,791,057 - -1.2 1,791,130 + -0.8 1,791,136 - +0.0 1,791,239 + +1.2 1,791,239 + -0.1
Or see this region's nucleotide sequence