Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07595 and RR42_RS07600 are separated by 13 nucleotides RR42_RS07600 and RR42_RS07605 are separated by 373 nucleotides RR42_RS07605 and RR42_RS07610 are separated by 76 nucleotides
RR42_RS07595: RR42_RS07595 - hypothetical protein, at 1,626,852 to 1,627,655
_RS07595
RR42_RS07600: RR42_RS07600 - membrane protein, at 1,627,669 to 1,627,929
_RS07600
RR42_RS07605: RR42_RS07605 - poly-beta-hydroxybutyrate polymerase, at 1,628,303 to 1,630,096
_RS07605
RR42_RS07610: RR42_RS07610 - acetyl-CoA acetyltransferase, at 1,630,173 to 1,631,354
_RS07610
Position (kb)
1628
1629
1630
1631 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.488 kb on + strand, within RR42_RS07595 at 1627.489 kb on - strand, within RR42_RS07595 at 1627.499 kb on + strand, within RR42_RS07595 at 1627.500 kb on - strand, within RR42_RS07595 at 1627.500 kb on - strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.503 kb on + strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.504 kb on - strand, within RR42_RS07595 at 1627.593 kb on + strand at 1627.593 kb on + strand at 1627.594 kb on - strand at 1627.600 kb on - strand at 1627.805 kb on + strand, within RR42_RS07600 at 1627.805 kb on + strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.806 kb on - strand, within RR42_RS07600 at 1627.835 kb on + strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600 at 1627.836 kb on - strand, within RR42_RS07600 at 1627.869 kb on + strand, within RR42_RS07600 at 1627.870 kb on - strand, within RR42_RS07600 at 1627.870 kb on - strand, within RR42_RS07600 at 1627.879 kb on - strand, within RR42_RS07600 at 1627.901 kb on + strand, within RR42_RS07600 at 1627.901 kb on + strand, within RR42_RS07600 at 1627.901 kb on + strand, within RR42_RS07600 at 1627.901 kb on + strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.902 kb on - strand, within RR42_RS07600 at 1627.925 kb on + strand at 1627.925 kb on + strand at 1627.925 kb on + strand at 1627.925 kb on + strand at 1627.925 kb on + strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1627.926 kb on - strand at 1628.058 kb on + strand at 1628.058 kb on + strand at 1628.170 kb on + strand at 1628.170 kb on + strand at 1628.276 kb on + strand at 1628.276 kb on + strand at 1628.276 kb on + strand at 1628.276 kb on + strand at 1628.276 kb on + strand at 1628.277 kb on - strand at 1628.277 kb on - strand at 1628.465 kb on - strand at 1628.465 kb on - strand at 1628.685 kb on - strand, within RR42_RS07605 at 1628.978 kb on + strand, within RR42_RS07605 at 1628.979 kb on - strand, within RR42_RS07605 at 1628.991 kb on - strand, within RR42_RS07605 at 1629.008 kb on + strand, within RR42_RS07605 at 1629.075 kb on - strand, within RR42_RS07605 at 1629.077 kb on + strand, within RR42_RS07605 at 1629.077 kb on + strand, within RR42_RS07605 at 1629.078 kb on - strand, within RR42_RS07605 at 1629.116 kb on + strand, within RR42_RS07605 at 1629.136 kb on + strand, within RR42_RS07605 at 1629.143 kb on + strand, within RR42_RS07605 at 1629.543 kb on - strand, within RR42_RS07605 at 1629.656 kb on - strand, within RR42_RS07605 at 1629.744 kb on - strand, within RR42_RS07605 at 1629.749 kb on + strand, within RR42_RS07605 at 1629.750 kb on - strand, within RR42_RS07605 at 1630.046 kb on + strand at 1630.095 kb on - strand at 1630.144 kb on - strand at 1630.192 kb on + strand at 1630.626 kb on + strand, within RR42_RS07610 at 1630.626 kb on + strand, within RR42_RS07610 at 1630.627 kb on - strand, within RR42_RS07610 at 1630.636 kb on - strand, within RR42_RS07610 at 1630.674 kb on + strand, within RR42_RS07610 at 1630.675 kb on - strand, within RR42_RS07610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,627,488 + RR42_RS07595 0.79 +0.0 1,627,488 + RR42_RS07595 0.79 -2.6 1,627,488 + RR42_RS07595 0.79 +0.4 1,627,489 - RR42_RS07595 0.79 +0.1 1,627,499 + RR42_RS07595 0.80 -0.1 1,627,500 - RR42_RS07595 0.81 -0.8 1,627,500 - RR42_RS07595 0.81 -0.1 1,627,503 + RR42_RS07595 0.81 -0.2 1,627,503 + RR42_RS07595 0.81 +0.1 1,627,503 + RR42_RS07595 0.81 +0.1 1,627,503 + RR42_RS07595 0.81 +0.1 1,627,503 + RR42_RS07595 0.81 -0.2 1,627,504 - RR42_RS07595 0.81 +0.2 1,627,504 - RR42_RS07595 0.81 +0.2 1,627,504 - RR42_RS07595 0.81 -0.6 1,627,504 - RR42_RS07595 0.81 -1.1 1,627,504 - RR42_RS07595 0.81 -0.4 1,627,593 + -1.1 1,627,593 + +0.3 1,627,594 - -0.5 1,627,600 - -0.3 1,627,805 + RR42_RS07600 0.52 -0.3 1,627,805 + RR42_RS07600 0.52 -0.1 1,627,806 - RR42_RS07600 0.52 -1.3 1,627,806 - RR42_RS07600 0.52 +0.7 1,627,806 - RR42_RS07600 0.52 -0.7 1,627,806 - RR42_RS07600 0.52 -0.9 1,627,806 - RR42_RS07600 0.52 +0.2 1,627,835 + RR42_RS07600 0.64 +2.2 1,627,836 - RR42_RS07600 0.64 +0.2 1,627,836 - RR42_RS07600 0.64 +1.1 1,627,836 - RR42_RS07600 0.64 -0.5 1,627,869 + RR42_RS07600 0.77 +0.0 1,627,870 - RR42_RS07600 0.77 +0.6 1,627,870 - RR42_RS07600 0.77 -1.0 1,627,879 - RR42_RS07600 0.80 +0.9 1,627,901 + RR42_RS07600 0.89 +0.4 1,627,901 + RR42_RS07600 0.89 -1.7 1,627,901 + RR42_RS07600 0.89 +0.3 1,627,901 + RR42_RS07600 0.89 +2.9 1,627,902 - RR42_RS07600 0.89 +0.7 1,627,902 - RR42_RS07600 0.89 +0.4 1,627,902 - RR42_RS07600 0.89 +0.2 1,627,902 - RR42_RS07600 0.89 -0.3 1,627,902 - RR42_RS07600 0.89 +0.8 1,627,902 - RR42_RS07600 0.89 -0.1 1,627,902 - RR42_RS07600 0.89 +0.2 1,627,902 - RR42_RS07600 0.89 -1.6 1,627,902 - RR42_RS07600 0.89 -0.4 1,627,902 - RR42_RS07600 0.89 +0.3 1,627,902 - RR42_RS07600 0.89 -0.0 1,627,902 - RR42_RS07600 0.89 -1.2 1,627,925 + -0.4 1,627,925 + -0.1 1,627,925 + +0.4 1,627,925 + +2.2 1,627,925 + +3.2 1,627,926 - -0.5 1,627,926 - -0.9 1,627,926 - +1.3 1,627,926 - +1.1 1,627,926 - +1.6 1,627,926 - -0.2 1,627,926 - -2.5 1,628,058 + -0.5 1,628,058 + -0.1 1,628,170 + -1.3 1,628,170 + +0.2 1,628,276 + -0.4 1,628,276 + -0.9 1,628,276 + -0.4 1,628,276 + +0.2 1,628,276 + -0.7 1,628,277 - -1.7 1,628,277 - -3.1 1,628,465 - -0.9 1,628,465 - -0.5 1,628,685 - RR42_RS07605 0.21 -1.7 1,628,978 + RR42_RS07605 0.38 -1.1 1,628,979 - RR42_RS07605 0.38 -0.1 1,628,991 - RR42_RS07605 0.38 +0.2 1,629,008 + RR42_RS07605 0.39 -2.1 1,629,075 - RR42_RS07605 0.43 -0.5 1,629,077 + RR42_RS07605 0.43 -0.2 1,629,077 + RR42_RS07605 0.43 -1.1 1,629,078 - RR42_RS07605 0.43 -1.9 1,629,116 + RR42_RS07605 0.45 -0.1 1,629,136 + RR42_RS07605 0.46 -2.1 1,629,143 + RR42_RS07605 0.47 +0.1 1,629,543 - RR42_RS07605 0.69 -1.0 1,629,656 - RR42_RS07605 0.75 +0.3 1,629,744 - RR42_RS07605 0.80 -1.1 1,629,749 + RR42_RS07605 0.81 +0.9 1,629,750 - RR42_RS07605 0.81 +0.6 1,630,046 + -2.6 1,630,095 - -2.5 1,630,144 - -1.3 1,630,192 + +0.0 1,630,626 + RR42_RS07610 0.38 -0.2 1,630,626 + RR42_RS07610 0.38 -0.3 1,630,627 - RR42_RS07610 0.38 -0.2 1,630,636 - RR42_RS07610 0.39 -0.7 1,630,674 + RR42_RS07610 0.42 -0.2 1,630,675 - RR42_RS07610 0.42 -1.0
Or see this region's nucleotide sequence