Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07215 and RR42_RS07220 are separated by 305 nucleotides RR42_RS07220 and RR42_RS07225 are separated by 295 nucleotides
RR42_RS07215: RR42_RS07215 - hypothetical protein, at 1,539,514 to 1,539,840
_RS07215
RR42_RS07220: RR42_RS07220 - hypothetical protein, at 1,540,146 to 1,540,331
_RS07220
RR42_RS07225: RR42_RS07225 - hypothetical protein, at 1,540,627 to 1,540,842
_RS07225
Position (kb)
1540
1541 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1539.665 kb on + strand, within RR42_RS07215 at 1539.689 kb on - strand, within RR42_RS07215 at 1539.759 kb on - strand, within RR42_RS07215 at 1539.853 kb on + strand at 1539.854 kb on + strand at 1539.854 kb on + strand at 1539.855 kb on - strand at 1539.874 kb on + strand at 1539.874 kb on + strand at 1540.050 kb on + strand at 1540.051 kb on - strand at 1540.051 kb on - strand at 1540.104 kb on - strand at 1540.104 kb on - strand at 1540.177 kb on - strand, within RR42_RS07220 at 1540.195 kb on - strand, within RR42_RS07220 at 1540.206 kb on - strand, within RR42_RS07220 at 1540.206 kb on - strand, within RR42_RS07220 at 1540.207 kb on + strand, within RR42_RS07220 at 1540.451 kb on + strand at 1540.451 kb on + strand at 1540.452 kb on - strand at 1540.466 kb on + strand at 1540.572 kb on + strand at 1540.573 kb on - strand at 1540.573 kb on - strand at 1540.715 kb on + strand, within RR42_RS07225 at 1540.894 kb on + strand at 1540.894 kb on + strand at 1540.894 kb on + strand at 1540.918 kb on + strand at 1541.243 kb on + strand at 1541.244 kb on - strand at 1541.273 kb on + strand at 1541.273 kb on + strand at 1541.273 kb on + strand at 1541.400 kb on - strand at 1541.444 kb on + strand at 1541.445 kb on - strand at 1541.445 kb on - strand at 1541.567 kb on - strand at 1541.567 kb on - strand at 1541.661 kb on + strand at 1541.662 kb on - strand at 1541.738 kb on + strand at 1541.750 kb on - strand at 1541.766 kb on - strand at 1541.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,539,665 + RR42_RS07215 0.46 +0.2 1,539,689 - RR42_RS07215 0.54 -2.7 1,539,759 - RR42_RS07215 0.75 +0.1 1,539,853 + +0.3 1,539,854 + -0.1 1,539,854 + -1.5 1,539,855 - -0.1 1,539,874 + +0.7 1,539,874 + -1.8 1,540,050 + -0.7 1,540,051 - -0.2 1,540,051 - -0.3 1,540,104 - -0.3 1,540,104 - +0.3 1,540,177 - RR42_RS07220 0.17 -0.5 1,540,195 - RR42_RS07220 0.26 +0.5 1,540,206 - RR42_RS07220 0.32 -0.7 1,540,206 - RR42_RS07220 0.32 -0.3 1,540,207 + RR42_RS07220 0.33 +0.8 1,540,451 + -0.3 1,540,451 + -0.2 1,540,452 - +0.3 1,540,466 + +0.7 1,540,572 + -0.0 1,540,573 - +0.2 1,540,573 - -0.9 1,540,715 + RR42_RS07225 0.41 -0.9 1,540,894 + +0.1 1,540,894 + +0.2 1,540,894 + +0.4 1,540,918 + +0.6 1,541,243 + +0.2 1,541,244 - +0.9 1,541,273 + -0.1 1,541,273 + +0.9 1,541,273 + +0.9 1,541,400 - -0.1 1,541,444 + -0.6 1,541,445 - +0.5 1,541,445 - +2.9 1,541,567 - +0.9 1,541,567 - -0.7 1,541,661 + -1.4 1,541,662 - -0.1 1,541,738 + +0.5 1,541,750 - +2.3 1,541,766 - +0.1 1,541,766 - -0.1
Or see this region's nucleotide sequence