Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07145 and RR42_RS07150 are separated by 27 nucleotides RR42_RS07150 and RR42_RS07155 are separated by 72 nucleotides RR42_RS07155 and RR42_RS07160 are separated by 59 nucleotides RR42_RS07160 and RR42_RS07165 are separated by 1 nucleotides
RR42_RS07145: RR42_RS07145 - hypothetical protein, at 1,530,968 to 1,532,830
_RS07145
RR42_RS07150: RR42_RS07150 - hypothetical protein, at 1,532,858 to 1,533,571
_RS07150
RR42_RS07155: RR42_RS07155 - hypothetical protein, at 1,533,644 to 1,534,405
_RS07155
RR42_RS07160: RR42_RS07160 - hypothetical protein, at 1,534,465 to 1,535,247
_RS07160
RR42_RS07165: RR42_RS07165 - hypothetical protein, at 1,535,249 to 1,535,467
_RS07165
Position (kb)
1533
1534
1535 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1532.768 kb on + strand at 1532.857 kb on + strand at 1533.195 kb on - strand, within RR42_RS07150 at 1533.633 kb on - strand at 1533.640 kb on - strand at 1533.640 kb on - strand at 1533.719 kb on + strand at 1533.720 kb on - strand at 1533.941 kb on - strand, within RR42_RS07155 at 1534.281 kb on - strand, within RR42_RS07155 at 1534.344 kb on - strand at 1534.429 kb on + strand at 1534.462 kb on - strand at 1534.690 kb on + strand, within RR42_RS07160 at 1534.756 kb on + strand, within RR42_RS07160 at 1534.756 kb on + strand, within RR42_RS07160 at 1534.757 kb on - strand, within RR42_RS07160 at 1534.809 kb on - strand, within RR42_RS07160 at 1535.052 kb on - strand, within RR42_RS07160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,532,768 + -2.6 1,532,857 + +0.9 1,533,195 - RR42_RS07150 0.47 -1.6 1,533,633 - -1.6 1,533,640 - +0.3 1,533,640 - +0.2 1,533,719 + -0.3 1,533,720 - -0.1 1,533,941 - RR42_RS07155 0.39 +0.6 1,534,281 - RR42_RS07155 0.84 +2.1 1,534,344 - -0.4 1,534,429 + -1.2 1,534,462 - +0.4 1,534,690 + RR42_RS07160 0.29 -1.0 1,534,756 + RR42_RS07160 0.37 -0.6 1,534,756 + RR42_RS07160 0.37 +1.1 1,534,757 - RR42_RS07160 0.37 +0.5 1,534,809 - RR42_RS07160 0.44 -0.5 1,535,052 - RR42_RS07160 0.75 -0.1
Or see this region's nucleotide sequence