Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07135 and RR42_RS07140 are separated by 183 nucleotides RR42_RS07140 and RR42_RS07145 are separated by 9 nucleotides
RR42_RS07135: RR42_RS07135 - hypothetical protein, at 1,528,616 to 1,530,304
_RS07135
RR42_RS07140: RR42_RS07140 - hypothetical protein, at 1,530,488 to 1,530,958
_RS07140
RR42_RS07145: RR42_RS07145 - hypothetical protein, at 1,530,968 to 1,532,830
_RS07145
Position (kb)
1530
1531 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1529.741 kb on + strand, within RR42_RS07135 at 1529.826 kb on - strand, within RR42_RS07135 at 1530.014 kb on + strand, within RR42_RS07135 at 1530.041 kb on + strand, within RR42_RS07135 at 1530.042 kb on - strand, within RR42_RS07135 at 1530.050 kb on + strand, within RR42_RS07135 at 1530.053 kb on + strand, within RR42_RS07135 at 1530.054 kb on - strand, within RR42_RS07135 at 1530.066 kb on + strand, within RR42_RS07135 at 1530.221 kb on + strand at 1530.221 kb on + strand at 1530.302 kb on + strand at 1530.317 kb on + strand at 1530.319 kb on - strand at 1530.535 kb on + strand, within RR42_RS07140 at 1530.718 kb on + strand, within RR42_RS07140 at 1530.957 kb on - strand at 1530.968 kb on - strand at 1530.968 kb on - strand at 1531.205 kb on + strand, within RR42_RS07145 at 1531.255 kb on + strand, within RR42_RS07145 at 1531.272 kb on + strand, within RR42_RS07145 at 1531.272 kb on + strand, within RR42_RS07145 at 1531.477 kb on - strand, within RR42_RS07145 at 1531.502 kb on + strand, within RR42_RS07145 at 1531.504 kb on + strand, within RR42_RS07145 at 1531.504 kb on + strand, within RR42_RS07145 at 1531.504 kb on + strand, within RR42_RS07145 at 1531.505 kb on - strand, within RR42_RS07145 at 1531.679 kb on - strand, within RR42_RS07145 at 1531.740 kb on - strand, within RR42_RS07145 at 1531.813 kb on - strand, within RR42_RS07145 at 1531.884 kb on - strand, within RR42_RS07145 at 1531.884 kb on - strand, within RR42_RS07145 at 1531.937 kb on + strand, within RR42_RS07145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,529,741 + RR42_RS07135 0.67 +2.1 1,529,826 - RR42_RS07135 0.72 +1.5 1,530,014 + RR42_RS07135 0.83 +0.0 1,530,041 + RR42_RS07135 0.84 -0.2 1,530,042 - RR42_RS07135 0.84 -1.1 1,530,050 + RR42_RS07135 0.85 -1.0 1,530,053 + RR42_RS07135 0.85 +0.5 1,530,054 - RR42_RS07135 0.85 -0.0 1,530,066 + RR42_RS07135 0.86 +0.5 1,530,221 + +1.0 1,530,221 + -0.4 1,530,302 + +0.2 1,530,317 + -1.4 1,530,319 - -1.7 1,530,535 + RR42_RS07140 0.10 -2.9 1,530,718 + RR42_RS07140 0.49 +0.6 1,530,957 - -0.1 1,530,968 - -0.1 1,530,968 - -0.9 1,531,205 + RR42_RS07145 0.13 -0.1 1,531,255 + RR42_RS07145 0.15 +0.5 1,531,272 + RR42_RS07145 0.16 -0.6 1,531,272 + RR42_RS07145 0.16 -0.6 1,531,477 - RR42_RS07145 0.27 -0.4 1,531,502 + RR42_RS07145 0.29 -0.4 1,531,504 + RR42_RS07145 0.29 -0.1 1,531,504 + RR42_RS07145 0.29 +1.1 1,531,504 + RR42_RS07145 0.29 -1.4 1,531,505 - RR42_RS07145 0.29 +0.8 1,531,679 - RR42_RS07145 0.38 +0.1 1,531,740 - RR42_RS07145 0.41 -0.1 1,531,813 - RR42_RS07145 0.45 -0.5 1,531,884 - RR42_RS07145 0.49 +0.9 1,531,884 - RR42_RS07145 0.49 -2.3 1,531,937 + RR42_RS07145 0.52 -1.4
Or see this region's nucleotide sequence