Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07120 and RR42_RS07125 are separated by 56 nucleotides RR42_RS07125 and RR42_RS07130 overlap by 8 nucleotides RR42_RS07130 and RR42_RS07135 are separated by 116 nucleotides
RR42_RS07120: RR42_RS07120 - hypothetical protein, at 1,526,499 to 1,527,764
_RS07120
RR42_RS07125: RR42_RS07125 - hypothetical protein, at 1,527,821 to 1,528,297
_RS07125
RR42_RS07130: RR42_RS07130 - hypothetical protein, at 1,528,290 to 1,528,499
_RS07130
RR42_RS07135: RR42_RS07135 - hypothetical protein, at 1,528,616 to 1,530,304
_RS07135
Position (kb)
1528
1529 Strain fitness (log2 ratio)
-2
-1
0
1 at 1527.370 kb on + strand, within RR42_RS07120 at 1527.407 kb on + strand, within RR42_RS07120 at 1527.605 kb on - strand, within RR42_RS07120 at 1527.605 kb on - strand, within RR42_RS07120 at 1527.666 kb on + strand at 1527.666 kb on + strand at 1527.680 kb on - strand at 1527.714 kb on + strand at 1527.714 kb on + strand at 1527.715 kb on - strand at 1527.715 kb on - strand at 1527.715 kb on - strand at 1527.776 kb on + strand at 1527.777 kb on - strand at 1527.777 kb on - strand at 1527.777 kb on - strand at 1527.863 kb on - strand at 1527.893 kb on + strand, within RR42_RS07125 at 1528.037 kb on - strand, within RR42_RS07125 at 1528.396 kb on - strand, within RR42_RS07130 at 1528.396 kb on - strand, within RR42_RS07130 at 1528.637 kb on + strand at 1528.637 kb on + strand at 1528.981 kb on + strand, within RR42_RS07135 at 1529.069 kb on + strand, within RR42_RS07135 at 1529.110 kb on + strand, within RR42_RS07135 at 1529.138 kb on + strand, within RR42_RS07135 at 1529.255 kb on + strand, within RR42_RS07135 at 1529.348 kb on + strand, within RR42_RS07135 at 1529.444 kb on + strand, within RR42_RS07135 at 1529.444 kb on + strand, within RR42_RS07135 at 1529.445 kb on - strand, within RR42_RS07135 at 1529.445 kb on - strand, within RR42_RS07135 at 1529.445 kb on - strand, within RR42_RS07135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,527,370 + RR42_RS07120 0.69 -0.6 1,527,407 + RR42_RS07120 0.72 -0.4 1,527,605 - RR42_RS07120 0.87 +0.3 1,527,605 - RR42_RS07120 0.87 -0.0 1,527,666 + +0.3 1,527,666 + +0.9 1,527,680 - +0.9 1,527,714 + -0.2 1,527,714 + -0.4 1,527,715 - -2.4 1,527,715 - +1.2 1,527,715 - -1.2 1,527,776 + -2.6 1,527,777 - +1.2 1,527,777 - +0.3 1,527,777 - +0.9 1,527,863 - +0.2 1,527,893 + RR42_RS07125 0.15 -0.7 1,528,037 - RR42_RS07125 0.45 +1.3 1,528,396 - RR42_RS07130 0.50 -0.9 1,528,396 - RR42_RS07130 0.50 -0.6 1,528,637 + -0.4 1,528,637 + +0.9 1,528,981 + RR42_RS07135 0.22 -0.2 1,529,069 + RR42_RS07135 0.27 -1.1 1,529,110 + RR42_RS07135 0.29 -0.2 1,529,138 + RR42_RS07135 0.31 +0.4 1,529,255 + RR42_RS07135 0.38 -1.2 1,529,348 + RR42_RS07135 0.43 -0.2 1,529,444 + RR42_RS07135 0.49 -1.2 1,529,444 + RR42_RS07135 0.49 -0.7 1,529,445 - RR42_RS07135 0.49 -0.2 1,529,445 - RR42_RS07135 0.49 -0.3 1,529,445 - RR42_RS07135 0.49 +0.2
Or see this region's nucleotide sequence