Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07050 and RR42_RS07055 are separated by 284 nucleotides RR42_RS07055 and RR42_RS07060 overlap by 4 nucleotides RR42_RS07060 and RR42_RS07065 are separated by 307 nucleotides
RR42_RS07050: RR42_RS07050 - hypothetical protein, at 1,511,150 to 1,511,752
_RS07050
RR42_RS07055: RR42_RS07055 - hypothetical protein, at 1,512,037 to 1,512,543
_RS07055
RR42_RS07060: RR42_RS07060 - hypothetical protein, at 1,512,540 to 1,512,755
_RS07060
RR42_RS07065: RR42_RS07065 - hypothetical protein, at 1,513,063 to 1,515,870
_RS07065
Position (kb)
1512
1513 Strain fitness (log2 ratio)
-2
-1
0
1 at 1511.547 kb on + strand, within RR42_RS07050 at 1511.547 kb on + strand, within RR42_RS07050 at 1511.548 kb on - strand, within RR42_RS07050 at 1511.585 kb on + strand, within RR42_RS07050 at 1511.585 kb on + strand, within RR42_RS07050 at 1511.681 kb on - strand, within RR42_RS07050 at 1511.681 kb on - strand, within RR42_RS07050 at 1511.681 kb on - strand, within RR42_RS07050 at 1511.681 kb on - strand, within RR42_RS07050 at 1511.681 kb on - strand, within RR42_RS07050 at 1511.725 kb on + strand at 1511.785 kb on + strand at 1511.786 kb on - strand at 1511.788 kb on + strand at 1511.789 kb on - strand at 1511.881 kb on - strand at 1512.033 kb on - strand at 1512.041 kb on + strand at 1512.041 kb on + strand at 1512.041 kb on + strand at 1512.042 kb on - strand at 1512.089 kb on + strand, within RR42_RS07055 at 1512.089 kb on + strand, within RR42_RS07055 at 1512.121 kb on + strand, within RR42_RS07055 at 1512.121 kb on + strand, within RR42_RS07055 at 1512.331 kb on + strand, within RR42_RS07055 at 1512.331 kb on + strand, within RR42_RS07055 at 1512.332 kb on - strand, within RR42_RS07055 at 1512.332 kb on - strand, within RR42_RS07055 at 1512.332 kb on - strand, within RR42_RS07055 at 1512.332 kb on - strand, within RR42_RS07055 at 1512.403 kb on + strand, within RR42_RS07055 at 1512.414 kb on - strand, within RR42_RS07055 at 1512.437 kb on - strand, within RR42_RS07055 at 1512.445 kb on - strand, within RR42_RS07055 at 1512.520 kb on + strand at 1512.520 kb on + strand at 1512.521 kb on - strand at 1512.521 kb on - strand at 1512.521 kb on - strand at 1512.596 kb on - strand, within RR42_RS07060 at 1512.619 kb on - strand, within RR42_RS07060 at 1512.619 kb on - strand, within RR42_RS07060 at 1512.719 kb on - strand, within RR42_RS07060 at 1512.756 kb on - strand at 1512.756 kb on - strand at 1512.756 kb on - strand at 1512.823 kb on + strand at 1512.823 kb on + strand at 1512.824 kb on - strand at 1512.833 kb on - strand at 1512.935 kb on - strand at 1512.984 kb on - strand at 1513.055 kb on + strand at 1513.152 kb on - strand at 1513.153 kb on + strand at 1513.154 kb on - strand at 1513.154 kb on - strand at 1513.297 kb on - strand at 1513.451 kb on - strand, within RR42_RS07065 at 1513.452 kb on + strand, within RR42_RS07065 at 1513.498 kb on - strand, within RR42_RS07065 at 1513.621 kb on + strand, within RR42_RS07065 at 1513.621 kb on + strand, within RR42_RS07065 at 1513.633 kb on + strand, within RR42_RS07065 at 1513.713 kb on + strand, within RR42_RS07065 at 1513.713 kb on + strand, within RR42_RS07065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,511,547 + RR42_RS07050 0.66 +0.3 1,511,547 + RR42_RS07050 0.66 +0.7 1,511,548 - RR42_RS07050 0.66 +0.3 1,511,585 + RR42_RS07050 0.72 -0.5 1,511,585 + RR42_RS07050 0.72 +1.6 1,511,681 - RR42_RS07050 0.88 +0.7 1,511,681 - RR42_RS07050 0.88 +0.5 1,511,681 - RR42_RS07050 0.88 +0.1 1,511,681 - RR42_RS07050 0.88 -2.0 1,511,681 - RR42_RS07050 0.88 -1.4 1,511,725 + +0.8 1,511,785 + -0.8 1,511,786 - +0.3 1,511,788 + -0.7 1,511,789 - -0.1 1,511,881 - -0.0 1,512,033 - +0.1 1,512,041 + +1.1 1,512,041 + +0.0 1,512,041 + -0.4 1,512,042 - -0.7 1,512,089 + RR42_RS07055 0.10 -0.8 1,512,089 + RR42_RS07055 0.10 -0.3 1,512,121 + RR42_RS07055 0.17 -1.1 1,512,121 + RR42_RS07055 0.17 +0.4 1,512,331 + RR42_RS07055 0.58 -0.9 1,512,331 + RR42_RS07055 0.58 +0.4 1,512,332 - RR42_RS07055 0.58 -0.5 1,512,332 - RR42_RS07055 0.58 +1.6 1,512,332 - RR42_RS07055 0.58 -0.5 1,512,332 - RR42_RS07055 0.58 -0.4 1,512,403 + RR42_RS07055 0.72 -1.0 1,512,414 - RR42_RS07055 0.74 -0.3 1,512,437 - RR42_RS07055 0.79 -0.8 1,512,445 - RR42_RS07055 0.80 -0.3 1,512,520 + +0.7 1,512,520 + +0.2 1,512,521 - +0.9 1,512,521 - +0.4 1,512,521 - -1.7 1,512,596 - RR42_RS07060 0.26 -0.3 1,512,619 - RR42_RS07060 0.37 -1.4 1,512,619 - RR42_RS07060 0.37 -0.5 1,512,719 - RR42_RS07060 0.83 -0.9 1,512,756 - -1.9 1,512,756 - -1.1 1,512,756 - -0.2 1,512,823 + -0.5 1,512,823 + +0.9 1,512,824 - -0.5 1,512,833 - -0.4 1,512,935 - +0.2 1,512,984 - -0.0 1,513,055 + -2.0 1,513,152 - +0.4 1,513,153 + +0.8 1,513,154 - +0.6 1,513,154 - -0.5 1,513,297 - +1.1 1,513,451 - RR42_RS07065 0.14 -0.0 1,513,452 + RR42_RS07065 0.14 -0.7 1,513,498 - RR42_RS07065 0.15 -0.9 1,513,621 + RR42_RS07065 0.20 +0.9 1,513,621 + RR42_RS07065 0.20 +0.7 1,513,633 + RR42_RS07065 0.20 +0.6 1,513,713 + RR42_RS07065 0.23 +1.4 1,513,713 + RR42_RS07065 0.23 +0.7
Or see this region's nucleotide sequence