Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06780 and RR42_RS06785 overlap by 4 nucleotides RR42_RS06785 and RR42_RS06790 overlap by 1 nucleotides RR42_RS06790 and RR42_RS06795 are separated by 5 nucleotides RR42_RS06795 and RR42_RS06800 are separated by 287 nucleotides
RR42_RS06780: RR42_RS06780 - hypothetical protein, at 1,468,984 to 1,470,300
_RS06780
RR42_RS06785: RR42_RS06785 - hypothetical protein, at 1,470,297 to 1,470,662
_RS06785
RR42_RS06790: RR42_RS06790 - hypothetical protein, at 1,470,662 to 1,472,362
_RS06790
RR42_RS06795: RR42_RS06795 - hypothetical protein, at 1,472,368 to 1,472,844
_RS06795
RR42_RS06800: RR42_RS06800 - hypothetical protein, at 1,473,132 to 1,474,007
_RS06800
Position (kb)
1470
1471
1472
1473 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1469.710 kb on - strand, within RR42_RS06780 at 1469.830 kb on - strand, within RR42_RS06780 at 1469.830 kb on - strand, within RR42_RS06780 at 1469.830 kb on - strand, within RR42_RS06780 at 1469.907 kb on + strand, within RR42_RS06780 at 1469.982 kb on - strand, within RR42_RS06780 at 1470.183 kb on + strand at 1470.196 kb on + strand at 1470.233 kb on + strand at 1470.234 kb on - strand at 1470.263 kb on + strand at 1470.401 kb on - strand, within RR42_RS06785 at 1470.404 kb on + strand, within RR42_RS06785 at 1470.405 kb on - strand, within RR42_RS06785 at 1470.405 kb on - strand, within RR42_RS06785 at 1470.660 kb on + strand at 1470.661 kb on - strand at 1470.677 kb on + strand at 1470.677 kb on + strand at 1470.678 kb on - strand at 1470.678 kb on - strand at 1470.678 kb on - strand at 1470.693 kb on + strand at 1470.957 kb on - strand, within RR42_RS06790 at 1470.957 kb on - strand, within RR42_RS06790 at 1471.085 kb on + strand, within RR42_RS06790 at 1471.086 kb on - strand, within RR42_RS06790 at 1471.226 kb on + strand, within RR42_RS06790 at 1471.304 kb on + strand, within RR42_RS06790 at 1471.304 kb on + strand, within RR42_RS06790 at 1471.304 kb on + strand, within RR42_RS06790 at 1471.305 kb on - strand, within RR42_RS06790 at 1471.323 kb on - strand, within RR42_RS06790 at 1471.433 kb on + strand, within RR42_RS06790 at 1471.434 kb on - strand, within RR42_RS06790 at 1471.566 kb on - strand, within RR42_RS06790 at 1471.817 kb on + strand, within RR42_RS06790 at 1471.817 kb on + strand, within RR42_RS06790 at 1471.880 kb on + strand, within RR42_RS06790 at 1471.880 kb on + strand, within RR42_RS06790 at 1471.925 kb on + strand, within RR42_RS06790 at 1471.926 kb on - strand, within RR42_RS06790 at 1471.963 kb on + strand, within RR42_RS06790 at 1472.058 kb on + strand, within RR42_RS06790 at 1472.059 kb on - strand, within RR42_RS06790 at 1472.059 kb on - strand, within RR42_RS06790 at 1472.153 kb on + strand, within RR42_RS06790 at 1472.153 kb on + strand, within RR42_RS06790 at 1472.154 kb on - strand, within RR42_RS06790 at 1472.154 kb on - strand, within RR42_RS06790 at 1472.622 kb on + strand, within RR42_RS06795 at 1472.622 kb on + strand, within RR42_RS06795 at 1472.622 kb on + strand, within RR42_RS06795 at 1472.623 kb on - strand, within RR42_RS06795 at 1472.692 kb on + strand, within RR42_RS06795 at 1472.693 kb on - strand, within RR42_RS06795 at 1472.693 kb on - strand, within RR42_RS06795 at 1472.781 kb on - strand, within RR42_RS06795 at 1472.801 kb on + strand at 1472.802 kb on - strand at 1472.843 kb on - strand at 1472.869 kb on + strand at 1472.870 kb on - strand at 1472.870 kb on - strand at 1472.870 kb on - strand at 1472.904 kb on + strand at 1472.905 kb on - strand at 1472.946 kb on + strand at 1472.947 kb on - strand at 1472.947 kb on - strand at 1472.961 kb on - strand at 1473.080 kb on + strand at 1473.080 kb on + strand at 1473.081 kb on - strand at 1473.172 kb on + strand at 1473.172 kb on + strand at 1473.172 kb on + strand at 1473.173 kb on - strand at 1473.267 kb on + strand, within RR42_RS06800 at 1473.268 kb on - strand, within RR42_RS06800 at 1473.268 kb on - strand, within RR42_RS06800 at 1473.268 kb on - strand, within RR42_RS06800 at 1473.268 kb on - strand, within RR42_RS06800 at 1473.269 kb on + strand, within RR42_RS06800 at 1473.269 kb on + strand, within RR42_RS06800 at 1473.270 kb on - strand, within RR42_RS06800 at 1473.270 kb on - strand, within RR42_RS06800 at 1473.270 kb on - strand, within RR42_RS06800 at 1473.270 kb on - strand, within RR42_RS06800 at 1473.270 kb on - strand, within RR42_RS06800 at 1473.321 kb on - strand, within RR42_RS06800 at 1473.343 kb on + strand, within RR42_RS06800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,469,710 - RR42_RS06780 0.55 +0.9 1,469,830 - RR42_RS06780 0.64 +0.4 1,469,830 - RR42_RS06780 0.64 +0.1 1,469,830 - RR42_RS06780 0.64 +0.1 1,469,907 + RR42_RS06780 0.70 +0.2 1,469,982 - RR42_RS06780 0.76 +0.2 1,470,183 + -0.5 1,470,196 + -0.0 1,470,233 + +3.2 1,470,234 - +0.7 1,470,263 + -0.1 1,470,401 - RR42_RS06785 0.28 -1.1 1,470,404 + RR42_RS06785 0.29 +0.6 1,470,405 - RR42_RS06785 0.30 +0.3 1,470,405 - RR42_RS06785 0.30 -0.1 1,470,660 + -0.2 1,470,661 - -0.4 1,470,677 + +1.0 1,470,677 + +0.5 1,470,678 - +0.4 1,470,678 - +0.3 1,470,678 - -2.4 1,470,693 + -0.1 1,470,957 - RR42_RS06790 0.17 +1.9 1,470,957 - RR42_RS06790 0.17 -0.0 1,471,085 + RR42_RS06790 0.25 +0.9 1,471,086 - RR42_RS06790 0.25 +0.6 1,471,226 + RR42_RS06790 0.33 -1.9 1,471,304 + RR42_RS06790 0.38 -1.5 1,471,304 + RR42_RS06790 0.38 -1.5 1,471,304 + RR42_RS06790 0.38 -0.3 1,471,305 - RR42_RS06790 0.38 +0.0 1,471,323 - RR42_RS06790 0.39 -0.8 1,471,433 + RR42_RS06790 0.45 +0.1 1,471,434 - RR42_RS06790 0.45 -1.2 1,471,566 - RR42_RS06790 0.53 -0.9 1,471,817 + RR42_RS06790 0.68 -0.5 1,471,817 + RR42_RS06790 0.68 -0.3 1,471,880 + RR42_RS06790 0.72 +0.9 1,471,880 + RR42_RS06790 0.72 -1.4 1,471,925 + RR42_RS06790 0.74 +0.1 1,471,926 - RR42_RS06790 0.74 -0.7 1,471,963 + RR42_RS06790 0.76 -1.8 1,472,058 + RR42_RS06790 0.82 -1.1 1,472,059 - RR42_RS06790 0.82 +0.7 1,472,059 - RR42_RS06790 0.82 -1.1 1,472,153 + RR42_RS06790 0.88 -1.0 1,472,153 + RR42_RS06790 0.88 -0.1 1,472,154 - RR42_RS06790 0.88 -0.5 1,472,154 - RR42_RS06790 0.88 +0.3 1,472,622 + RR42_RS06795 0.53 +0.4 1,472,622 + RR42_RS06795 0.53 -0.1 1,472,622 + RR42_RS06795 0.53 +0.1 1,472,623 - RR42_RS06795 0.53 +0.2 1,472,692 + RR42_RS06795 0.68 +0.1 1,472,693 - RR42_RS06795 0.68 +1.2 1,472,693 - RR42_RS06795 0.68 -1.0 1,472,781 - RR42_RS06795 0.87 +0.3 1,472,801 + +0.3 1,472,802 - -0.6 1,472,843 - -0.3 1,472,869 + +0.7 1,472,870 - -0.0 1,472,870 - -0.5 1,472,870 - +0.4 1,472,904 + -0.2 1,472,905 - +0.0 1,472,946 + -0.4 1,472,947 - +0.8 1,472,947 - +0.6 1,472,961 - +0.0 1,473,080 + +0.3 1,473,080 + +0.0 1,473,081 - +0.3 1,473,172 + +0.9 1,473,172 + +0.2 1,473,172 + -0.8 1,473,173 - -0.0 1,473,267 + RR42_RS06800 0.15 -1.4 1,473,268 - RR42_RS06800 0.16 -0.5 1,473,268 - RR42_RS06800 0.16 -0.7 1,473,268 - RR42_RS06800 0.16 +0.5 1,473,268 - RR42_RS06800 0.16 +0.2 1,473,269 + RR42_RS06800 0.16 -0.9 1,473,269 + RR42_RS06800 0.16 +1.7 1,473,270 - RR42_RS06800 0.16 -1.1 1,473,270 - RR42_RS06800 0.16 -0.6 1,473,270 - RR42_RS06800 0.16 -0.6 1,473,270 - RR42_RS06800 0.16 -0.7 1,473,270 - RR42_RS06800 0.16 +1.4 1,473,321 - RR42_RS06800 0.22 -0.5 1,473,343 + RR42_RS06800 0.24 +0.6
Or see this region's nucleotide sequence