Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06645 and RR42_RS06650 are separated by 265 nucleotides RR42_RS06650 and RR42_RS06655 overlap by 13 nucleotides RR42_RS06655 and RR42_RS06660 are separated by 135 nucleotides RR42_RS06660 and RR42_RS06665 are separated by 47 nucleotides
RR42_RS06645: RR42_RS06645 - hypothetical protein, at 1,449,333 to 1,450,430
_RS06645
RR42_RS06650: RR42_RS06650 - hypothetical protein, at 1,450,696 to 1,451,268
_RS06650
RR42_RS06655: RR42_RS06655 - hypothetical protein, at 1,451,256 to 1,451,453
_RS06655
RR42_RS06660: RR42_RS06660 - hypothetical protein, at 1,451,589 to 1,451,771
_RS06660
RR42_RS06665: RR42_RS06665 - reverse transcriptase, at 1,451,819 to 1,452,994
_RS06665
Position (kb)
1451
1452 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1450.363 kb on + strand at 1450.363 kb on + strand at 1450.364 kb on - strand at 1450.364 kb on - strand at 1450.366 kb on + strand at 1450.366 kb on + strand at 1450.367 kb on - strand at 1450.524 kb on + strand at 1450.544 kb on + strand at 1450.551 kb on - strand at 1450.556 kb on - strand at 1450.556 kb on - strand at 1450.556 kb on - strand at 1450.679 kb on + strand at 1450.679 kb on + strand at 1450.679 kb on + strand at 1450.680 kb on - strand at 1450.680 kb on - strand at 1450.680 kb on - strand at 1450.680 kb on - strand at 1450.683 kb on - strand at 1450.763 kb on + strand, within RR42_RS06650 at 1450.763 kb on + strand, within RR42_RS06650 at 1450.779 kb on - strand, within RR42_RS06650 at 1450.779 kb on - strand, within RR42_RS06650 at 1450.866 kb on + strand, within RR42_RS06650 at 1450.867 kb on - strand at 1450.898 kb on + strand, within RR42_RS06650 at 1450.898 kb on + strand, within RR42_RS06650 at 1450.899 kb on - strand, within RR42_RS06650 at 1450.899 kb on - strand, within RR42_RS06650 at 1450.899 kb on - strand, within RR42_RS06650 at 1450.978 kb on + strand, within RR42_RS06650 at 1450.979 kb on - strand, within RR42_RS06650 at 1451.087 kb on + strand, within RR42_RS06650 at 1451.088 kb on - strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.169 kb on + strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.170 kb on - strand, within RR42_RS06650 at 1451.179 kb on + strand, within RR42_RS06650 at 1451.270 kb on + strand at 1451.270 kb on + strand at 1451.270 kb on + strand at 1451.271 kb on - strand at 1451.271 kb on - strand at 1451.271 kb on - strand at 1451.271 kb on - strand at 1451.271 kb on - strand at 1451.307 kb on + strand, within RR42_RS06655 at 1451.308 kb on + strand, within RR42_RS06655 at 1451.308 kb on + strand, within RR42_RS06655 at 1451.309 kb on - strand, within RR42_RS06655 at 1451.410 kb on + strand, within RR42_RS06655 at 1451.446 kb on + strand at 1451.446 kb on + strand at 1451.447 kb on - strand at 1451.447 kb on - strand at 1451.461 kb on - strand at 1451.527 kb on - strand at 1451.590 kb on + strand at 1451.591 kb on - strand at 1451.680 kb on + strand, within RR42_RS06660 at 1451.797 kb on + strand at 1451.797 kb on + strand at 1451.798 kb on - strand at 1451.798 kb on - strand at 1451.798 kb on - strand at 1451.798 kb on - strand at 1451.798 kb on - strand at 1451.827 kb on + strand at 1451.827 kb on + strand at 1451.828 kb on - strand at 1451.838 kb on + strand at 1451.838 kb on + strand at 1451.839 kb on - strand at 1451.849 kb on + strand at 1451.849 kb on + strand at 1451.946 kb on + strand, within RR42_RS06665 at 1451.946 kb on + strand, within RR42_RS06665 at 1451.966 kb on + strand, within RR42_RS06665 at 1451.966 kb on + strand, within RR42_RS06665 at 1451.967 kb on - strand, within RR42_RS06665 at 1452.035 kb on + strand, within RR42_RS06665 at 1452.089 kb on + strand, within RR42_RS06665 at 1452.089 kb on + strand, within RR42_RS06665 at 1452.211 kb on - strand, within RR42_RS06665 at 1452.220 kb on - strand, within RR42_RS06665 at 1452.220 kb on - strand, within RR42_RS06665 at 1452.338 kb on + strand, within RR42_RS06665 at 1452.338 kb on + strand, within RR42_RS06665 at 1452.339 kb on - strand, within RR42_RS06665 at 1452.347 kb on - strand, within RR42_RS06665 at 1452.349 kb on - strand, within RR42_RS06665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,450,363 + -0.1 1,450,363 + +1.3 1,450,364 - -0.5 1,450,364 - +0.1 1,450,366 + -0.5 1,450,366 + -0.3 1,450,367 - +0.2 1,450,524 + -0.1 1,450,544 + +0.6 1,450,551 - +0.6 1,450,556 - -1.4 1,450,556 - -0.2 1,450,556 - -0.4 1,450,679 + -0.3 1,450,679 + +0.1 1,450,679 + +0.9 1,450,680 - -0.1 1,450,680 - +0.1 1,450,680 - -0.5 1,450,680 - +0.2 1,450,683 - +0.5 1,450,763 + RR42_RS06650 0.12 -0.7 1,450,763 + RR42_RS06650 0.12 +0.5 1,450,779 - RR42_RS06650 0.14 -0.7 1,450,779 - RR42_RS06650 0.14 -0.8 1,450,866 + RR42_RS06650 0.30 -0.3 1,450,867 - +0.9 1,450,898 + RR42_RS06650 0.35 +0.4 1,450,898 + RR42_RS06650 0.35 -0.5 1,450,899 - RR42_RS06650 0.35 -0.5 1,450,899 - RR42_RS06650 0.35 +0.7 1,450,899 - RR42_RS06650 0.35 +0.2 1,450,978 + RR42_RS06650 0.49 -0.7 1,450,979 - RR42_RS06650 0.49 +0.1 1,451,087 + RR42_RS06650 0.68 -0.1 1,451,088 - RR42_RS06650 0.68 -0.2 1,451,169 + RR42_RS06650 0.83 -0.9 1,451,169 + RR42_RS06650 0.83 -0.5 1,451,169 + RR42_RS06650 0.83 +0.5 1,451,169 + RR42_RS06650 0.83 -0.7 1,451,169 + RR42_RS06650 0.83 -0.7 1,451,169 + RR42_RS06650 0.83 -0.3 1,451,169 + RR42_RS06650 0.83 +0.2 1,451,170 - RR42_RS06650 0.83 -0.6 1,451,170 - RR42_RS06650 0.83 -0.9 1,451,170 - RR42_RS06650 0.83 -0.5 1,451,170 - RR42_RS06650 0.83 -0.3 1,451,170 - RR42_RS06650 0.83 -0.2 1,451,170 - RR42_RS06650 0.83 +0.3 1,451,170 - RR42_RS06650 0.83 +0.6 1,451,170 - RR42_RS06650 0.83 -0.3 1,451,170 - RR42_RS06650 0.83 -0.5 1,451,170 - RR42_RS06650 0.83 +0.6 1,451,179 + RR42_RS06650 0.84 -0.1 1,451,270 + +0.5 1,451,270 + +0.8 1,451,270 + -0.3 1,451,271 - -0.3 1,451,271 - -1.4 1,451,271 - -0.7 1,451,271 - +0.5 1,451,271 - +0.3 1,451,307 + RR42_RS06655 0.26 -1.4 1,451,308 + RR42_RS06655 0.26 -1.1 1,451,308 + RR42_RS06655 0.26 -1.9 1,451,309 - RR42_RS06655 0.27 +0.7 1,451,410 + RR42_RS06655 0.78 +0.0 1,451,446 + -1.2 1,451,446 + -0.5 1,451,447 - -0.1 1,451,447 - -0.7 1,451,461 - -0.3 1,451,527 - -0.6 1,451,590 + +0.2 1,451,591 - +0.9 1,451,680 + RR42_RS06660 0.50 +1.8 1,451,797 + +0.3 1,451,797 + -1.4 1,451,798 - -0.1 1,451,798 - -0.8 1,451,798 - -0.2 1,451,798 - -0.0 1,451,798 - -1.0 1,451,827 + -0.3 1,451,827 + -0.1 1,451,828 - +0.0 1,451,838 + -0.3 1,451,838 + -1.9 1,451,839 - -2.1 1,451,849 + -0.1 1,451,849 + +0.9 1,451,946 + RR42_RS06665 0.11 -0.5 1,451,946 + RR42_RS06665 0.11 +0.9 1,451,966 + RR42_RS06665 0.12 -0.1 1,451,966 + RR42_RS06665 0.12 -1.1 1,451,967 - RR42_RS06665 0.13 -0.4 1,452,035 + RR42_RS06665 0.18 +0.5 1,452,089 + RR42_RS06665 0.23 -0.1 1,452,089 + RR42_RS06665 0.23 -0.3 1,452,211 - RR42_RS06665 0.33 +2.3 1,452,220 - RR42_RS06665 0.34 +0.0 1,452,220 - RR42_RS06665 0.34 +0.4 1,452,338 + RR42_RS06665 0.44 -0.9 1,452,338 + RR42_RS06665 0.44 +0.6 1,452,339 - RR42_RS06665 0.44 +0.9 1,452,347 - RR42_RS06665 0.45 -1.4 1,452,349 - RR42_RS06665 0.45 -1.1
Or see this region's nucleotide sequence