Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06370 and RR42_RS06375 are separated by 150 nucleotides RR42_RS06375 and RR42_RS06380 are separated by 277 nucleotides RR42_RS06380 and RR42_RS06385 are separated by 155 nucleotides
RR42_RS06370: RR42_RS06370 - aspartate kinase, at 1,397,226 to 1,398,476
_RS06370
RR42_RS06375: RR42_RS06375 - tRNA-Ser, at 1,398,627 to 1,398,720
_RS06375
RR42_RS06380: RR42_RS06380 - hypothetical protein, at 1,398,998 to 1,399,390
_RS06380
RR42_RS06385: RR42_RS06385 - N-ethylmaleimide reductase, at 1,399,546 to 1,400,658
_RS06385
Position (kb)
1398
1399 Strain fitness (log2 ratio)
-2
-1
0
1 at 1398.449 kb on + strand at 1398.449 kb on + strand at 1398.450 kb on - strand at 1398.450 kb on - strand at 1398.450 kb on - strand at 1398.450 kb on - strand at 1398.538 kb on + strand at 1398.722 kb on + strand at 1398.730 kb on + strand at 1398.824 kb on + strand at 1398.824 kb on + strand at 1398.825 kb on - strand at 1398.825 kb on - strand at 1398.856 kb on + strand at 1398.856 kb on + strand at 1399.186 kb on + strand, within RR42_RS06380 at 1399.257 kb on + strand, within RR42_RS06380 at 1399.257 kb on + strand, within RR42_RS06380 at 1399.257 kb on + strand, within RR42_RS06380 at 1399.258 kb on - strand, within RR42_RS06380 at 1399.400 kb on + strand at 1399.400 kb on + strand at 1399.401 kb on - strand at 1399.401 kb on - strand at 1399.530 kb on + strand at 1399.562 kb on + strand at 1399.571 kb on + strand at 1399.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,398,449 + -1.8 1,398,449 + +0.3 1,398,450 - -0.4 1,398,450 - +0.3 1,398,450 - +0.2 1,398,450 - -0.8 1,398,538 + -0.5 1,398,722 + +0.5 1,398,730 + -2.6 1,398,824 + +0.0 1,398,824 + -0.4 1,398,825 - -1.5 1,398,825 - +0.3 1,398,856 + -0.1 1,398,856 + +0.6 1,399,186 + RR42_RS06380 0.48 -2.4 1,399,257 + RR42_RS06380 0.66 +0.6 1,399,257 + RR42_RS06380 0.66 -0.3 1,399,257 + RR42_RS06380 0.66 +1.1 1,399,258 - RR42_RS06380 0.66 +0.2 1,399,400 + +0.5 1,399,400 + -0.6 1,399,401 - +0.3 1,399,401 - -0.0 1,399,530 + +1.7 1,399,562 + -0.9 1,399,571 + -0.5 1,399,572 - +0.4
Or see this region's nucleotide sequence