Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06300 and RR42_RS06305 are separated by 155 nucleotides RR42_RS06305 and RR42_RS06310 are separated by 154 nucleotides RR42_RS06310 and RR42_RS06315 are separated by 137 nucleotides
RR42_RS06300: RR42_RS06300 - DNA-binding protein, at 1,383,264 to 1,384,739
_RS06300
RR42_RS06305: RR42_RS06305 - hypothetical protein, at 1,384,895 to 1,385,530
_RS06305
RR42_RS06310: RR42_RS06310 - inositol monophosphatase, at 1,385,685 to 1,386,506
_RS06310
RR42_RS06315: RR42_RS06315 - RNA methyltransferase, at 1,386,644 to 1,387,645
_RS06315
Position (kb)
1385
1386
1387 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1384.715 kb on + strand at 1384.776 kb on + strand at 1384.824 kb on - strand at 1384.962 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.001 kb on - strand, within RR42_RS06305 at 1385.081 kb on + strand, within RR42_RS06305 at 1385.081 kb on + strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.082 kb on - strand, within RR42_RS06305 at 1385.207 kb on + strand, within RR42_RS06305 at 1385.207 kb on + strand, within RR42_RS06305 at 1385.225 kb on + strand, within RR42_RS06305 at 1385.226 kb on - strand, within RR42_RS06305 at 1385.226 kb on - strand, within RR42_RS06305 at 1385.516 kb on + strand at 1385.517 kb on - strand at 1385.517 kb on - strand at 1385.517 kb on - strand at 1385.686 kb on + strand at 1386.659 kb on + strand at 1386.660 kb on - strand at 1386.661 kb on + strand at 1386.662 kb on - strand at 1386.662 kb on - strand at 1387.014 kb on + strand, within RR42_RS06315 at 1387.014 kb on + strand, within RR42_RS06315 at 1387.014 kb on + strand, within RR42_RS06315 at 1387.015 kb on - strand, within RR42_RS06315 at 1387.071 kb on - strand, within RR42_RS06315 at 1387.301 kb on + strand, within RR42_RS06315 at 1387.400 kb on + strand, within RR42_RS06315 at 1387.401 kb on - strand, within RR42_RS06315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,384,715 + +1.4 1,384,776 + -0.2 1,384,824 - -0.4 1,384,962 - RR42_RS06305 0.11 +0.5 1,385,001 - RR42_RS06305 0.17 -0.3 1,385,001 - RR42_RS06305 0.17 +1.1 1,385,001 - RR42_RS06305 0.17 -2.9 1,385,001 - RR42_RS06305 0.17 +0.9 1,385,081 + RR42_RS06305 0.29 +0.6 1,385,081 + RR42_RS06305 0.29 +0.8 1,385,082 - RR42_RS06305 0.29 -0.3 1,385,082 - RR42_RS06305 0.29 +1.0 1,385,082 - RR42_RS06305 0.29 +0.1 1,385,082 - RR42_RS06305 0.29 -0.3 1,385,082 - RR42_RS06305 0.29 -0.9 1,385,207 + RR42_RS06305 0.49 +0.5 1,385,207 + RR42_RS06305 0.49 -0.2 1,385,225 + RR42_RS06305 0.52 -0.5 1,385,226 - RR42_RS06305 0.52 +0.6 1,385,226 - RR42_RS06305 0.52 -0.1 1,385,516 + -0.4 1,385,517 - -0.0 1,385,517 - +0.1 1,385,517 - +0.6 1,385,686 + +2.0 1,386,659 + +0.8 1,386,660 - +0.4 1,386,661 + +0.8 1,386,662 - +0.0 1,386,662 - +2.0 1,387,014 + RR42_RS06315 0.37 +0.8 1,387,014 + RR42_RS06315 0.37 -0.0 1,387,014 + RR42_RS06315 0.37 +1.3 1,387,015 - RR42_RS06315 0.37 +0.1 1,387,071 - RR42_RS06315 0.43 +0.8 1,387,301 + RR42_RS06315 0.66 -1.4 1,387,400 + RR42_RS06315 0.75 +0.9 1,387,401 - RR42_RS06315 0.76 +1.4
Or see this region's nucleotide sequence