Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06050 and RR42_RS06055 are separated by 42 nucleotides RR42_RS06055 and RR42_RS06060 are separated by 66 nucleotides RR42_RS06060 and RR42_RS06065 are separated by 61 nucleotides RR42_RS06065 and RR42_RS06070 are separated by 271 nucleotides
RR42_RS06050: RR42_RS06050 - molecular chaperone HscA, at 1,327,631 to 1,329,493
_RS06050
RR42_RS06055: RR42_RS06055 - (2Fe-2S) ferredoxin, at 1,329,536 to 1,329,874
_RS06055
RR42_RS06060: RR42_RS06060 - hypothetical protein, at 1,329,941 to 1,330,135
_RS06060
RR42_RS06065: RR42_RS06065 - hypothetical protein, at 1,330,197 to 1,330,979
_RS06065
RR42_RS06070: RR42_RS06070 - lysyl-tRNA synthetase, at 1,331,251 to 1,332,789
_RS06070
Position (kb)
1330
1331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1329.515 kb on + strand at 1329.959 kb on + strand at 1329.959 kb on - strand at 1330.134 kb on - strand at 1330.268 kb on - strand at 1330.315 kb on + strand, within RR42_RS06065 at 1330.500 kb on + strand, within RR42_RS06065 at 1330.501 kb on - strand, within RR42_RS06065 at 1330.507 kb on - strand, within RR42_RS06065 at 1330.591 kb on + strand, within RR42_RS06065 at 1330.979 kb on + strand at 1330.979 kb on + strand at 1330.979 kb on + strand at 1331.041 kb on + strand at 1331.041 kb on + strand at 1331.041 kb on + strand at 1331.041 kb on + strand at 1331.042 kb on - strand at 1331.091 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,329,515 + -2.5 1,329,959 + -0.4 1,329,959 - -1.3 1,330,134 - -2.8 1,330,268 - -0.1 1,330,315 + RR42_RS06065 0.15 +0.7 1,330,500 + RR42_RS06065 0.39 -0.3 1,330,501 - RR42_RS06065 0.39 +0.2 1,330,507 - RR42_RS06065 0.40 +2.1 1,330,591 + RR42_RS06065 0.50 +0.3 1,330,979 + -0.9 1,330,979 + +1.4 1,330,979 + -0.1 1,331,041 + -0.4 1,331,041 + +0.5 1,331,041 + +1.5 1,331,041 + -0.6 1,331,042 - -0.1 1,331,091 + +1.2
Or see this region's nucleotide sequence