Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06015 and RR42_RS06020 are separated by 80 nucleotides RR42_RS06020 and RR42_RS06025 are separated by 68 nucleotides
RR42_RS06015: RR42_RS06015 - recombinase, at 1,321,784 to 1,323,874
_RS06015
RR42_RS06020: RR42_RS06020 - Fe-S cluster assembly transcriptional regulator IscR, at 1,323,955 to 1,324,509
_RS06020
RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795
_RS06025
Position (kb)
1323
1324
1325 Strain fitness (log2 ratio)
-2
-1
0
1 at 1322.996 kb on + strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1322.997 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.134 kb on - strand, within RR42_RS06015 at 1323.331 kb on - strand, within RR42_RS06015 at 1323.410 kb on + strand, within RR42_RS06015 at 1323.410 kb on + strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.411 kb on - strand, within RR42_RS06015 at 1323.428 kb on + strand, within RR42_RS06015 at 1323.428 kb on + strand, within RR42_RS06015 at 1323.429 kb on - strand, within RR42_RS06015 at 1323.481 kb on + strand, within RR42_RS06015 at 1323.481 kb on + strand, within RR42_RS06015 at 1323.482 kb on - strand, within RR42_RS06015 at 1323.482 kb on - strand, within RR42_RS06015 at 1324.248 kb on + strand, within RR42_RS06020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,322,996 + RR42_RS06015 0.58 -1.6 1,322,997 - RR42_RS06015 0.58 -0.2 1,322,997 - RR42_RS06015 0.58 -2.5 1,322,997 - RR42_RS06015 0.58 +0.5 1,322,997 - RR42_RS06015 0.58 -0.0 1,322,997 - RR42_RS06015 0.58 -0.6 1,323,134 - RR42_RS06015 0.65 +0.5 1,323,134 - RR42_RS06015 0.65 +0.0 1,323,134 - RR42_RS06015 0.65 +0.4 1,323,134 - RR42_RS06015 0.65 -0.1 1,323,134 - RR42_RS06015 0.65 -0.6 1,323,331 - RR42_RS06015 0.74 -0.4 1,323,410 + RR42_RS06015 0.78 +0.3 1,323,410 + RR42_RS06015 0.78 -0.6 1,323,411 - RR42_RS06015 0.78 +0.2 1,323,411 - RR42_RS06015 0.78 -1.9 1,323,411 - RR42_RS06015 0.78 -0.6 1,323,428 + RR42_RS06015 0.79 +0.6 1,323,428 + RR42_RS06015 0.79 -0.6 1,323,429 - RR42_RS06015 0.79 +1.3 1,323,481 + RR42_RS06015 0.81 +0.8 1,323,481 + RR42_RS06015 0.81 -1.3 1,323,482 - RR42_RS06015 0.81 -0.8 1,323,482 - RR42_RS06015 0.81 -1.0 1,324,248 + RR42_RS06020 0.53 -0.8
Or see this region's nucleotide sequence