Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05745 and RR42_RS05750 are separated by 117 nucleotides RR42_RS05750 and RR42_RS05755 are separated by 105 nucleotides RR42_RS05755 and RR42_RS05760 are separated by 92 nucleotides
RR42_RS05745: RR42_RS05745 - aminoglycoside phosphotransferase, at 1,264,143 to 1,265,219
_RS05745
RR42_RS05750: RR42_RS05750 - fructose 2,6-bisphosphatase, at 1,265,337 to 1,266,071
_RS05750
RR42_RS05755: RR42_RS05755 - enoyl-CoA hydratase, at 1,266,177 to 1,266,956
_RS05755
RR42_RS05760: RR42_RS05760 - 3-hydroxyacyl-CoA dehydrogenase, at 1,267,049 to 1,268,572
_RS05760
Position (kb)
1266
1267 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1265.266 kb on + strand at 1265.267 kb on - strand at 1265.267 kb on - strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.319 kb on + strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.320 kb on - strand at 1265.338 kb on + strand at 1265.374 kb on + strand at 1265.375 kb on - strand at 1265.440 kb on + strand, within RR42_RS05750 at 1266.010 kb on + strand at 1266.010 kb on + strand at 1266.011 kb on - strand at 1266.037 kb on + strand at 1266.038 kb on - strand at 1266.178 kb on + strand at 1266.862 kb on + strand, within RR42_RS05755 at 1266.862 kb on + strand, within RR42_RS05755 at 1266.863 kb on - strand, within RR42_RS05755 at 1266.863 kb on - strand, within RR42_RS05755 at 1267.050 kb on + strand at 1267.050 kb on + strand at 1267.164 kb on - strand at 1267.164 kb on - strand at 1267.213 kb on - strand, within RR42_RS05760 at 1267.476 kb on - strand, within RR42_RS05760 at 1267.593 kb on - strand, within RR42_RS05760 at 1267.723 kb on - strand, within RR42_RS05760 at 1267.810 kb on - strand, within RR42_RS05760 at 1267.821 kb on + strand, within RR42_RS05760 at 1267.821 kb on + strand, within RR42_RS05760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,265,266 + +0.2 1,265,267 - -0.3 1,265,267 - -0.1 1,265,319 + -0.4 1,265,319 + +0.0 1,265,319 + +1.8 1,265,319 + -0.4 1,265,319 + +0.2 1,265,319 + -0.4 1,265,319 + +0.2 1,265,320 - -0.4 1,265,320 - -0.7 1,265,320 - +0.5 1,265,320 - +1.0 1,265,338 + -0.8 1,265,374 + +0.4 1,265,375 - +0.5 1,265,440 + RR42_RS05750 0.14 -0.5 1,266,010 + -0.9 1,266,010 + +0.5 1,266,011 - -2.1 1,266,037 + -0.3 1,266,038 - -0.8 1,266,178 + +0.2 1,266,862 + RR42_RS05755 0.88 -1.8 1,266,862 + RR42_RS05755 0.88 -0.6 1,266,863 - RR42_RS05755 0.88 +2.6 1,266,863 - RR42_RS05755 0.88 -2.3 1,267,050 + +0.1 1,267,050 + -0.3 1,267,164 - -0.7 1,267,164 - +0.4 1,267,213 - RR42_RS05760 0.11 -0.2 1,267,476 - RR42_RS05760 0.28 +0.3 1,267,593 - RR42_RS05760 0.36 -0.1 1,267,723 - RR42_RS05760 0.44 +1.0 1,267,810 - RR42_RS05760 0.50 +0.5 1,267,821 + RR42_RS05760 0.51 +0.3 1,267,821 + RR42_RS05760 0.51 +0.6
Or see this region's nucleotide sequence