Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05655 and RR42_RS05660 are separated by 66 nucleotides RR42_RS05660 and RR42_RS05665 are separated by 44 nucleotides RR42_RS05665 and RR42_RS05670 are separated by 28 nucleotides RR42_RS05670 and RR42_RS05675 overlap by 1 nucleotides
RR42_RS05655: RR42_RS05655 - urease accessory protein UreD, at 1,245,645 to 1,246,517
_RS05655
RR42_RS05660: RR42_RS05660 - urease subunit gamma, at 1,246,584 to 1,246,886
_RS05660
RR42_RS05665: RR42_RS05665 - urease accessory protein UreJ, at 1,246,931 to 1,247,563
_RS05665
RR42_RS05670: RR42_RS05670 - urease subunit beta, at 1,247,592 to 1,247,897
_RS05670
RR42_RS05675: RR42_RS05675 - urease subunit alpha, at 1,247,897 to 1,249,615
_RS05675
Position (kb) 1246
1247
1248 Strain fitness (log2 ratio) -1
0
1 at 1246.283 kb on - strand, within RR42_RS05655 at 1246.516 kb on - strand at 1246.516 kb on - strand at 1247.418 kb on - strand, within RR42_RS05665 at 1247.778 kb on - strand, within RR42_RS05670 at 1247.863 kb on - strand, within RR42_RS05670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,246,283 - RR42_RS05655 0.73 +0.2 1,246,516 - +0.4 1,246,516 - -0.6 1,247,418 - RR42_RS05665 0.77 -1.0 1,247,778 - RR42_RS05670 0.61 +0.6 1,247,863 - RR42_RS05670 0.89 -0.1
Or see this region's nucleotide sequence