Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05385 and RR42_RS05390 overlap by 4 nucleotides RR42_RS05390 and RR42_RS05395 are separated by 139 nucleotides RR42_RS05395 and RR42_RS05400 are separated by 4 nucleotides
RR42_RS05385: RR42_RS05385 - LOG family protein, at 1,191,084 to 1,191,668
_RS05385
RR42_RS05390: RR42_RS05390 - TetR family transcriptional regulator, at 1,191,665 to 1,192,363
_RS05390
RR42_RS05395: RR42_RS05395 - DeoR family transcriptional regulator, at 1,192,503 to 1,192,898
_RS05395
RR42_RS05400: RR42_RS05400 - GDP-mannose-dependent alpha-mannosyltransferase, at 1,192,903 to 1,194,069
_RS05400
Position (kb)
1192
1193 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1191.557 kb on - strand, within RR42_RS05385 at 1191.649 kb on + strand at 1191.649 kb on + strand at 1191.650 kb on - strand at 1191.650 kb on - strand at 1191.778 kb on + strand, within RR42_RS05390 at 1191.778 kb on + strand, within RR42_RS05390 at 1191.778 kb on + strand, within RR42_RS05390 at 1191.778 kb on + strand, within RR42_RS05390 at 1191.778 kb on + strand, within RR42_RS05390 at 1191.779 kb on - strand, within RR42_RS05390 at 1191.779 kb on - strand, within RR42_RS05390 at 1191.783 kb on + strand, within RR42_RS05390 at 1191.784 kb on - strand, within RR42_RS05390 at 1191.784 kb on - strand, within RR42_RS05390 at 1191.829 kb on - strand, within RR42_RS05390 at 1191.829 kb on - strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.867 kb on + strand, within RR42_RS05390 at 1191.868 kb on - strand, within RR42_RS05390 at 1191.868 kb on - strand, within RR42_RS05390 at 1191.886 kb on + strand, within RR42_RS05390 at 1191.886 kb on + strand, within RR42_RS05390 at 1191.887 kb on - strand, within RR42_RS05390 at 1192.086 kb on + strand, within RR42_RS05390 at 1192.086 kb on + strand, within RR42_RS05390 at 1192.086 kb on + strand, within RR42_RS05390 at 1192.086 kb on + strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.087 kb on - strand, within RR42_RS05390 at 1192.096 kb on - strand, within RR42_RS05390 at 1192.215 kb on + strand, within RR42_RS05390 at 1192.218 kb on + strand, within RR42_RS05390 at 1192.406 kb on - strand at 1192.407 kb on + strand at 1192.408 kb on - strand at 1192.572 kb on + strand, within RR42_RS05395 at 1192.912 kb on + strand at 1192.913 kb on - strand at 1192.913 kb on - strand at 1192.913 kb on - strand at 1193.311 kb on - strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.351 kb on + strand, within RR42_RS05400 at 1193.352 kb on - strand, within RR42_RS05400 at 1193.380 kb on + strand, within RR42_RS05400 at 1193.381 kb on - strand, within RR42_RS05400 at 1193.391 kb on - strand, within RR42_RS05400 at 1193.391 kb on - strand, within RR42_RS05400 at 1193.520 kb on - strand, within RR42_RS05400 at 1193.553 kb on + strand, within RR42_RS05400 at 1193.554 kb on - strand, within RR42_RS05400 at 1193.702 kb on + strand, within RR42_RS05400 at 1193.703 kb on - strand, within RR42_RS05400 at 1193.741 kb on + strand, within RR42_RS05400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,191,557 - RR42_RS05385 0.81 -0.6 1,191,649 + +0.0 1,191,649 + -0.5 1,191,650 - -0.2 1,191,650 - +1.0 1,191,778 + RR42_RS05390 0.16 -1.1 1,191,778 + RR42_RS05390 0.16 +0.2 1,191,778 + RR42_RS05390 0.16 -0.1 1,191,778 + RR42_RS05390 0.16 +0.1 1,191,778 + RR42_RS05390 0.16 +0.1 1,191,779 - RR42_RS05390 0.16 -0.2 1,191,779 - RR42_RS05390 0.16 +0.6 1,191,783 + RR42_RS05390 0.17 -1.1 1,191,784 - RR42_RS05390 0.17 -2.0 1,191,784 - RR42_RS05390 0.17 -1.4 1,191,829 - RR42_RS05390 0.23 -1.1 1,191,829 - RR42_RS05390 0.23 +0.3 1,191,867 + RR42_RS05390 0.29 +0.5 1,191,867 + RR42_RS05390 0.29 +0.6 1,191,867 + RR42_RS05390 0.29 +1.0 1,191,867 + RR42_RS05390 0.29 -0.2 1,191,867 + RR42_RS05390 0.29 +0.0 1,191,867 + RR42_RS05390 0.29 -0.3 1,191,868 - RR42_RS05390 0.29 -0.9 1,191,868 - RR42_RS05390 0.29 -2.1 1,191,886 + RR42_RS05390 0.32 -1.4 1,191,886 + RR42_RS05390 0.32 +0.9 1,191,887 - RR42_RS05390 0.32 -1.2 1,192,086 + RR42_RS05390 0.60 -0.3 1,192,086 + RR42_RS05390 0.60 -0.8 1,192,086 + RR42_RS05390 0.60 +2.0 1,192,086 + RR42_RS05390 0.60 -0.2 1,192,087 - RR42_RS05390 0.60 -1.0 1,192,087 - RR42_RS05390 0.60 -1.5 1,192,087 - RR42_RS05390 0.60 -0.7 1,192,087 - RR42_RS05390 0.60 -0.2 1,192,087 - RR42_RS05390 0.60 -1.1 1,192,087 - RR42_RS05390 0.60 +0.2 1,192,087 - RR42_RS05390 0.60 +0.6 1,192,087 - RR42_RS05390 0.60 -0.9 1,192,087 - RR42_RS05390 0.60 +0.2 1,192,087 - RR42_RS05390 0.60 +0.7 1,192,096 - RR42_RS05390 0.62 -0.5 1,192,215 + RR42_RS05390 0.79 +0.7 1,192,218 + RR42_RS05390 0.79 +0.5 1,192,406 - -0.2 1,192,407 + -0.3 1,192,408 - -0.4 1,192,572 + RR42_RS05395 0.17 -0.4 1,192,912 + +0.6 1,192,913 - -0.6 1,192,913 - -0.6 1,192,913 - +0.4 1,193,311 - RR42_RS05400 0.35 +0.3 1,193,351 + RR42_RS05400 0.38 +0.9 1,193,351 + RR42_RS05400 0.38 -1.8 1,193,351 + RR42_RS05400 0.38 -0.9 1,193,351 + RR42_RS05400 0.38 -0.4 1,193,352 - RR42_RS05400 0.38 +1.4 1,193,380 + RR42_RS05400 0.41 -0.9 1,193,381 - RR42_RS05400 0.41 -1.1 1,193,391 - RR42_RS05400 0.42 +0.2 1,193,391 - RR42_RS05400 0.42 -1.3 1,193,520 - RR42_RS05400 0.53 -0.1 1,193,553 + RR42_RS05400 0.56 -0.2 1,193,554 - RR42_RS05400 0.56 +0.0 1,193,702 + RR42_RS05400 0.68 -0.5 1,193,703 - RR42_RS05400 0.69 +0.6 1,193,741 + RR42_RS05400 0.72 +0.2
Or see this region's nucleotide sequence