Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05330 and RR42_RS05335 are separated by 112 nucleotides RR42_RS05335 and RR42_RS05340 are separated by 12 nucleotides
RR42_RS05330: RR42_RS05330 - xanthine dehydrogenase, at 1,177,868 to 1,178,902
_RS05330
RR42_RS05335: RR42_RS05335 - aldehyde oxidase, at 1,179,015 to 1,181,372
_RS05335
RR42_RS05340: RR42_RS05340 - FAD-binding molybdopterin dehydrogenase, at 1,181,385 to 1,182,899
_RS05340
Position (kb)
1179
1180
1181
1182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1178.142 kb on + strand, within RR42_RS05330 at 1178.220 kb on + strand, within RR42_RS05330 at 1178.221 kb on - strand, within RR42_RS05330 at 1179.013 kb on + strand at 1179.016 kb on + strand at 1179.017 kb on - strand at 1179.017 kb on - strand at 1179.017 kb on - strand at 1179.017 kb on - strand at 1179.104 kb on - strand at 1179.235 kb on + strand at 1179.235 kb on + strand at 1179.236 kb on - strand at 1179.236 kb on - strand at 1179.236 kb on - strand at 1179.275 kb on - strand, within RR42_RS05335 at 1179.556 kb on + strand, within RR42_RS05335 at 1179.556 kb on + strand, within RR42_RS05335 at 1179.557 kb on - strand, within RR42_RS05335 at 1179.557 kb on - strand, within RR42_RS05335 at 1179.592 kb on + strand, within RR42_RS05335 at 1179.592 kb on + strand, within RR42_RS05335 at 1179.809 kb on + strand, within RR42_RS05335 at 1179.941 kb on - strand, within RR42_RS05335 at 1179.992 kb on + strand, within RR42_RS05335 at 1179.993 kb on - strand, within RR42_RS05335 at 1180.081 kb on + strand, within RR42_RS05335 at 1180.243 kb on + strand, within RR42_RS05335 at 1180.243 kb on + strand, within RR42_RS05335 at 1180.244 kb on - strand, within RR42_RS05335 at 1180.244 kb on - strand, within RR42_RS05335 at 1180.244 kb on - strand, within RR42_RS05335 at 1180.352 kb on - strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.787 kb on - strand, within RR42_RS05335 at 1180.894 kb on + strand, within RR42_RS05335 at 1180.894 kb on + strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1181.242 kb on + strand at 1181.242 kb on + strand at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.768 kb on + strand, within RR42_RS05340 at 1181.845 kb on + strand, within RR42_RS05340 at 1181.845 kb on + strand, within RR42_RS05340 at 1181.854 kb on + strand, within RR42_RS05340 at 1181.854 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.149 kb on - strand, within RR42_RS05340 at 1182.149 kb on - strand, within RR42_RS05340 at 1182.277 kb on + strand, within RR42_RS05340 at 1182.278 kb on - strand, within RR42_RS05340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,178,142 + RR42_RS05330 0.26 -0.6 1,178,220 + RR42_RS05330 0.34 -0.3 1,178,221 - RR42_RS05330 0.34 -0.2 1,179,013 + -0.4 1,179,016 + -0.0 1,179,017 - -0.5 1,179,017 - -0.7 1,179,017 - -0.7 1,179,017 - -0.1 1,179,104 - +1.5 1,179,235 + -1.0 1,179,235 + -0.1 1,179,236 - +0.2 1,179,236 - +0.9 1,179,236 - +0.5 1,179,275 - RR42_RS05335 0.11 -0.2 1,179,556 + RR42_RS05335 0.23 -3.1 1,179,556 + RR42_RS05335 0.23 -0.0 1,179,557 - RR42_RS05335 0.23 -0.4 1,179,557 - RR42_RS05335 0.23 +0.0 1,179,592 + RR42_RS05335 0.24 -1.8 1,179,592 + RR42_RS05335 0.24 -0.3 1,179,809 + RR42_RS05335 0.34 -0.2 1,179,941 - RR42_RS05335 0.39 -2.1 1,179,992 + RR42_RS05335 0.41 -0.8 1,179,993 - RR42_RS05335 0.41 -0.4 1,180,081 + RR42_RS05335 0.45 +0.6 1,180,243 + RR42_RS05335 0.52 +1.0 1,180,243 + RR42_RS05335 0.52 -0.9 1,180,244 - RR42_RS05335 0.52 +0.5 1,180,244 - RR42_RS05335 0.52 +0.4 1,180,244 - RR42_RS05335 0.52 -0.3 1,180,352 - RR42_RS05335 0.57 +3.0 1,180,682 + RR42_RS05335 0.71 -0.1 1,180,682 + RR42_RS05335 0.71 +1.0 1,180,682 + RR42_RS05335 0.71 -0.1 1,180,682 + RR42_RS05335 0.71 -2.7 1,180,682 + RR42_RS05335 0.71 -0.6 1,180,787 - RR42_RS05335 0.75 +0.4 1,180,894 + RR42_RS05335 0.80 +0.5 1,180,894 + RR42_RS05335 0.80 -0.5 1,180,895 - RR42_RS05335 0.80 -1.7 1,180,895 - RR42_RS05335 0.80 -0.5 1,180,895 - RR42_RS05335 0.80 +0.2 1,181,242 + -0.4 1,181,242 + +0.8 1,181,764 + RR42_RS05340 0.25 +0.5 1,181,764 + RR42_RS05340 0.25 -0.9 1,181,764 + RR42_RS05340 0.25 -0.5 1,181,764 + RR42_RS05340 0.25 +0.2 1,181,765 - RR42_RS05340 0.25 -0.8 1,181,765 - RR42_RS05340 0.25 -0.6 1,181,765 - RR42_RS05340 0.25 -0.4 1,181,768 + RR42_RS05340 0.25 +0.4 1,181,845 + RR42_RS05340 0.30 +0.7 1,181,845 + RR42_RS05340 0.30 +0.1 1,181,854 + RR42_RS05340 0.31 -1.0 1,181,854 + RR42_RS05340 0.31 -1.0 1,182,148 + RR42_RS05340 0.50 -1.0 1,182,148 + RR42_RS05340 0.50 -0.6 1,182,148 + RR42_RS05340 0.50 +0.4 1,182,148 + RR42_RS05340 0.50 -0.5 1,182,149 - RR42_RS05340 0.50 +0.0 1,182,149 - RR42_RS05340 0.50 -2.9 1,182,277 + RR42_RS05340 0.59 -0.8 1,182,278 - RR42_RS05340 0.59 -2.0
Or see this region's nucleotide sequence