Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04740 and RR42_RS04745 are separated by 438 nucleotides RR42_RS04745 and RR42_RS04750 are separated by 114 nucleotides
RR42_RS04740: RR42_RS04740 - ATP-binding protein, at 1,054,984 to 1,056,534
_RS04740
RR42_RS04745: RR42_RS04745 - uracil phosphoribosyltransferase, at 1,056,973 to 1,057,623
_RS04745
RR42_RS04750: RR42_RS04750 - ABC transporter substrate-binding protein, at 1,057,738 to 1,058,739
_RS04750
Position (kb)
1056
1057
1058 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1056.202 kb on + strand, within RR42_RS04740 at 1056.202 kb on + strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.203 kb on - strand, within RR42_RS04740 at 1056.461 kb on + strand at 1056.532 kb on + strand at 1056.532 kb on + strand at 1056.533 kb on - strand at 1056.533 kb on - strand at 1056.533 kb on - strand at 1056.552 kb on + strand at 1056.553 kb on - strand at 1056.868 kb on + strand at 1056.869 kb on - strand at 1056.927 kb on + strand at 1056.967 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.028 kb on + strand at 1057.029 kb on - strand at 1057.029 kb on - strand at 1057.029 kb on - strand at 1057.099 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.123 kb on + strand, within RR42_RS04745 at 1057.124 kb on - strand, within RR42_RS04745 at 1057.140 kb on + strand, within RR42_RS04745 at 1057.141 kb on - strand, within RR42_RS04745 at 1057.199 kb on + strand, within RR42_RS04745 at 1057.200 kb on - strand, within RR42_RS04745 at 1057.230 kb on + strand, within RR42_RS04745 at 1057.231 kb on - strand, within RR42_RS04745 at 1057.231 kb on - strand, within RR42_RS04745 at 1057.268 kb on + strand, within RR42_RS04745 at 1057.268 kb on + strand, within RR42_RS04745 at 1057.269 kb on - strand, within RR42_RS04745 at 1057.269 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.309 kb on - strand, within RR42_RS04745 at 1057.482 kb on - strand, within RR42_RS04745 at 1057.592 kb on + strand at 1057.592 kb on + strand at 1057.592 kb on + strand at 1057.592 kb on + strand at 1057.592 kb on + strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.593 kb on - strand at 1057.673 kb on + strand at 1057.673 kb on + strand at 1057.673 kb on + strand at 1057.674 kb on - strand at 1057.674 kb on - strand at 1057.867 kb on + strand, within RR42_RS04750 at 1057.868 kb on - strand, within RR42_RS04750 at 1057.946 kb on + strand, within RR42_RS04750 at 1057.947 kb on - strand, within RR42_RS04750 at 1057.974 kb on - strand, within RR42_RS04750 at 1058.140 kb on - strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.159 kb on + strand, within RR42_RS04750 at 1058.160 kb on - strand, within RR42_RS04750 at 1058.160 kb on - strand, within RR42_RS04750 at 1058.160 kb on - strand, within RR42_RS04750 at 1058.160 kb on - strand, within RR42_RS04750 at 1058.160 kb on - strand, within RR42_RS04750 at 1058.247 kb on - strand, within RR42_RS04750 at 1058.247 kb on - strand, within RR42_RS04750 at 1058.273 kb on + strand, within RR42_RS04750 at 1058.274 kb on - strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.396 kb on + strand, within RR42_RS04750 at 1058.397 kb on - strand, within RR42_RS04750 at 1058.400 kb on + strand, within RR42_RS04750 at 1058.400 kb on + strand, within RR42_RS04750 at 1058.401 kb on - strand, within RR42_RS04750 at 1058.401 kb on - strand, within RR42_RS04750 at 1058.401 kb on - strand, within RR42_RS04750 at 1058.488 kb on - strand, within RR42_RS04750 at 1058.597 kb on + strand, within RR42_RS04750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,056,202 + RR42_RS04740 0.79 +0.1 1,056,202 + RR42_RS04740 0.79 -2.7 1,056,203 - RR42_RS04740 0.79 -0.4 1,056,203 - RR42_RS04740 0.79 -0.4 1,056,203 - RR42_RS04740 0.79 -0.0 1,056,203 - RR42_RS04740 0.79 +0.8 1,056,203 - RR42_RS04740 0.79 -1.0 1,056,203 - RR42_RS04740 0.79 +0.3 1,056,203 - RR42_RS04740 0.79 -0.8 1,056,461 + +1.1 1,056,532 + +0.7 1,056,532 + +0.5 1,056,533 - -0.5 1,056,533 - -0.0 1,056,533 - +1.8 1,056,552 + -1.4 1,056,553 - -0.5 1,056,868 + +0.3 1,056,869 - +0.4 1,056,927 + +0.3 1,056,967 + -0.2 1,057,028 + +0.3 1,057,028 + +1.1 1,057,028 + -0.9 1,057,028 + +0.8 1,057,028 + +1.0 1,057,028 + +0.4 1,057,028 + -0.4 1,057,028 + -0.9 1,057,029 - -0.8 1,057,029 - +0.4 1,057,029 - -0.3 1,057,099 + RR42_RS04745 0.19 +0.1 1,057,123 + RR42_RS04745 0.23 +0.7 1,057,123 + RR42_RS04745 0.23 -0.2 1,057,123 + RR42_RS04745 0.23 -0.5 1,057,123 + RR42_RS04745 0.23 -0.6 1,057,123 + RR42_RS04745 0.23 +0.1 1,057,124 - RR42_RS04745 0.23 -0.5 1,057,140 + RR42_RS04745 0.26 -0.3 1,057,141 - RR42_RS04745 0.26 +0.5 1,057,199 + RR42_RS04745 0.35 +1.6 1,057,200 - RR42_RS04745 0.35 +1.1 1,057,230 + RR42_RS04745 0.39 +1.5 1,057,231 - RR42_RS04745 0.40 -2.4 1,057,231 - RR42_RS04745 0.40 -2.9 1,057,268 + RR42_RS04745 0.45 +0.7 1,057,268 + RR42_RS04745 0.45 -1.2 1,057,269 - RR42_RS04745 0.45 -0.1 1,057,269 - RR42_RS04745 0.45 -0.6 1,057,309 - RR42_RS04745 0.52 -0.9 1,057,309 - RR42_RS04745 0.52 -2.1 1,057,309 - RR42_RS04745 0.52 +0.3 1,057,309 - RR42_RS04745 0.52 +0.8 1,057,482 - RR42_RS04745 0.78 -1.3 1,057,592 + +0.3 1,057,592 + +1.3 1,057,592 + -1.9 1,057,592 + +0.2 1,057,592 + +0.8 1,057,593 - -1.5 1,057,593 - +0.2 1,057,593 - -1.0 1,057,593 - +0.4 1,057,593 - +0.4 1,057,593 - +0.1 1,057,593 - -0.5 1,057,673 + +0.8 1,057,673 + +1.5 1,057,673 + +0.8 1,057,674 - +0.3 1,057,674 - +0.7 1,057,867 + RR42_RS04750 0.13 +2.2 1,057,868 - RR42_RS04750 0.13 -0.3 1,057,946 + RR42_RS04750 0.21 +0.3 1,057,947 - RR42_RS04750 0.21 -0.5 1,057,974 - RR42_RS04750 0.24 +0.5 1,058,140 - RR42_RS04750 0.40 +0.2 1,058,159 + RR42_RS04750 0.42 +0.5 1,058,159 + RR42_RS04750 0.42 +0.4 1,058,159 + RR42_RS04750 0.42 -0.4 1,058,159 + RR42_RS04750 0.42 +1.6 1,058,159 + RR42_RS04750 0.42 +0.2 1,058,159 + RR42_RS04750 0.42 +2.4 1,058,160 - RR42_RS04750 0.42 +0.4 1,058,160 - RR42_RS04750 0.42 -0.5 1,058,160 - RR42_RS04750 0.42 -0.2 1,058,160 - RR42_RS04750 0.42 +0.1 1,058,160 - RR42_RS04750 0.42 +0.4 1,058,247 - RR42_RS04750 0.51 +0.4 1,058,247 - RR42_RS04750 0.51 -0.6 1,058,273 + RR42_RS04750 0.53 -0.8 1,058,274 - RR42_RS04750 0.53 +0.2 1,058,396 + RR42_RS04750 0.66 -0.0 1,058,396 + RR42_RS04750 0.66 +0.1 1,058,396 + RR42_RS04750 0.66 -1.1 1,058,396 + RR42_RS04750 0.66 +0.1 1,058,396 + RR42_RS04750 0.66 +1.8 1,058,396 + RR42_RS04750 0.66 +0.3 1,058,397 - RR42_RS04750 0.66 +0.6 1,058,400 + RR42_RS04750 0.66 +0.2 1,058,400 + RR42_RS04750 0.66 +0.1 1,058,401 - RR42_RS04750 0.66 -0.6 1,058,401 - RR42_RS04750 0.66 +0.7 1,058,401 - RR42_RS04750 0.66 +0.3 1,058,488 - RR42_RS04750 0.75 +0.5 1,058,597 + RR42_RS04750 0.86 +0.6
Or see this region's nucleotide sequence