Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04600 and RR42_RS04605 are separated by 38 nucleotides RR42_RS04605 and RR42_RS04610 are separated by 281 nucleotides RR42_RS04610 and RR42_RS04615 are separated by 159 nucleotides
RR42_RS04600: RR42_RS04600 - LysR family transcriptional regulator, at 1,026,354 to 1,027,280
_RS04600
RR42_RS04605: RR42_RS04605 - membrane protein, at 1,027,319 to 1,027,804
_RS04605
RR42_RS04610: RR42_RS04610 - hypothetical protein, at 1,028,086 to 1,028,493
_RS04610
RR42_RS04615: RR42_RS04615 - sorbosone dehydrogenase, at 1,028,653 to 1,029,786
_RS04615
Position (kb)
1028
1029 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1027.158 kb on + strand, within RR42_RS04600 at 1027.158 kb on + strand, within RR42_RS04600 at 1027.159 kb on - strand, within RR42_RS04600 at 1027.320 kb on + strand at 1027.320 kb on + strand at 1027.321 kb on - strand at 1027.515 kb on + strand, within RR42_RS04605 at 1027.516 kb on - strand, within RR42_RS04605 at 1027.516 kb on - strand, within RR42_RS04605 at 1027.516 kb on - strand, within RR42_RS04605 at 1027.544 kb on + strand, within RR42_RS04605 at 1027.544 kb on + strand, within RR42_RS04605 at 1027.544 kb on + strand, within RR42_RS04605 at 1027.544 kb on + strand, within RR42_RS04605 at 1027.686 kb on + strand, within RR42_RS04605 at 1027.689 kb on + strand, within RR42_RS04605 at 1027.689 kb on + strand, within RR42_RS04605 at 1027.690 kb on - strand, within RR42_RS04605 at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.816 kb on + strand at 1027.817 kb on - strand at 1027.817 kb on - strand at 1027.817 kb on - strand at 1027.817 kb on - strand at 1027.822 kb on - strand at 1027.893 kb on + strand at 1027.893 kb on + strand at 1027.893 kb on + strand at 1027.894 kb on - strand at 1027.894 kb on - strand at 1027.894 kb on - strand at 1027.894 kb on - strand at 1027.894 kb on - strand at 1028.008 kb on + strand at 1028.008 kb on + strand at 1028.009 kb on - strand at 1028.009 kb on - strand at 1028.009 kb on - strand at 1028.009 kb on - strand at 1028.172 kb on + strand, within RR42_RS04610 at 1028.172 kb on + strand, within RR42_RS04610 at 1028.312 kb on - strand, within RR42_RS04610 at 1028.385 kb on + strand, within RR42_RS04610 at 1028.385 kb on + strand, within RR42_RS04610 at 1028.386 kb on - strand, within RR42_RS04610 at 1028.386 kb on - strand, within RR42_RS04610 at 1028.386 kb on - strand, within RR42_RS04610 at 1028.386 kb on - strand, within RR42_RS04610 at 1028.506 kb on + strand at 1028.507 kb on - strand at 1028.537 kb on - strand at 1028.550 kb on - strand at 1028.577 kb on + strand at 1028.577 kb on + strand at 1028.578 kb on - strand at 1028.578 kb on - strand at 1028.578 kb on - strand at 1028.632 kb on + strand at 1028.927 kb on + strand, within RR42_RS04615 at 1028.927 kb on + strand, within RR42_RS04615 at 1029.044 kb on + strand, within RR42_RS04615 at 1029.044 kb on + strand, within RR42_RS04615 at 1029.045 kb on - strand, within RR42_RS04615 at 1029.045 kb on - strand, within RR42_RS04615 at 1029.273 kb on + strand, within RR42_RS04615 at 1029.273 kb on + strand, within RR42_RS04615 at 1029.274 kb on - strand, within RR42_RS04615 at 1029.274 kb on - strand, within RR42_RS04615 at 1029.299 kb on + strand, within RR42_RS04615 at 1029.299 kb on + strand, within RR42_RS04615 at 1029.299 kb on + strand, within RR42_RS04615 at 1029.306 kb on - strand, within RR42_RS04615 at 1029.371 kb on - strand, within RR42_RS04615 at 1029.473 kb on - strand, within RR42_RS04615
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,027,158 + RR42_RS04600 0.87 -1.2 1,027,158 + RR42_RS04600 0.87 -1.2 1,027,159 - RR42_RS04600 0.87 -0.6 1,027,320 + -0.8 1,027,320 + +0.3 1,027,321 - -0.3 1,027,515 + RR42_RS04605 0.40 +0.1 1,027,516 - RR42_RS04605 0.41 -0.4 1,027,516 - RR42_RS04605 0.41 +0.3 1,027,516 - RR42_RS04605 0.41 -1.4 1,027,544 + RR42_RS04605 0.46 -0.3 1,027,544 + RR42_RS04605 0.46 -2.5 1,027,544 + RR42_RS04605 0.46 -0.1 1,027,544 + RR42_RS04605 0.46 +0.5 1,027,686 + RR42_RS04605 0.76 +1.1 1,027,689 + RR42_RS04605 0.76 -1.1 1,027,689 + RR42_RS04605 0.76 -0.7 1,027,690 - RR42_RS04605 0.76 +0.1 1,027,816 + -0.2 1,027,816 + -1.7 1,027,816 + +1.2 1,027,816 + -0.0 1,027,816 + -1.3 1,027,816 + -0.1 1,027,816 + -0.2 1,027,816 + +0.1 1,027,817 - -0.6 1,027,817 - -0.8 1,027,817 - +0.1 1,027,817 - +1.1 1,027,822 - -2.0 1,027,893 + -0.9 1,027,893 + -0.6 1,027,893 + -0.1 1,027,894 - +0.0 1,027,894 - +0.1 1,027,894 - +0.5 1,027,894 - -1.6 1,027,894 - -0.8 1,028,008 + +1.1 1,028,008 + +1.1 1,028,009 - +1.4 1,028,009 - -0.3 1,028,009 - -0.8 1,028,009 - -0.5 1,028,172 + RR42_RS04610 0.21 +0.7 1,028,172 + RR42_RS04610 0.21 -0.7 1,028,312 - RR42_RS04610 0.55 +1.1 1,028,385 + RR42_RS04610 0.73 -0.5 1,028,385 + RR42_RS04610 0.73 -1.3 1,028,386 - RR42_RS04610 0.74 -1.5 1,028,386 - RR42_RS04610 0.74 -0.4 1,028,386 - RR42_RS04610 0.74 -1.2 1,028,386 - RR42_RS04610 0.74 -0.5 1,028,506 + -0.8 1,028,507 - -1.2 1,028,537 - +0.0 1,028,550 - -0.4 1,028,577 + +1.1 1,028,577 + +1.1 1,028,578 - -0.2 1,028,578 - -0.2 1,028,578 - -1.2 1,028,632 + +0.1 1,028,927 + RR42_RS04615 0.24 +1.1 1,028,927 + RR42_RS04615 0.24 +0.6 1,029,044 + RR42_RS04615 0.34 -0.2 1,029,044 + RR42_RS04615 0.34 +0.3 1,029,045 - RR42_RS04615 0.35 +1.1 1,029,045 - RR42_RS04615 0.35 +1.1 1,029,273 + RR42_RS04615 0.55 -1.2 1,029,273 + RR42_RS04615 0.55 -0.0 1,029,274 - RR42_RS04615 0.55 +1.1 1,029,274 - RR42_RS04615 0.55 +0.8 1,029,299 + RR42_RS04615 0.57 -1.5 1,029,299 + RR42_RS04615 0.57 +0.4 1,029,299 + RR42_RS04615 0.57 -0.6 1,029,306 - RR42_RS04615 0.58 -0.6 1,029,371 - RR42_RS04615 0.63 +3.7 1,029,473 - RR42_RS04615 0.72 +1.0
Or see this region's nucleotide sequence