Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04520 and RR42_RS04525 are separated by 200 nucleotides RR42_RS04525 and RR42_RS04530 are separated by 284 nucleotides RR42_RS04530 and RR42_RS04535 are separated by 70 nucleotides
RR42_RS04520: RR42_RS04520 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 1,009,490 to 1,010,485
_RS04520
RR42_RS04525: RR42_RS04525 - hypothetical protein, at 1,010,686 to 1,011,048
_RS04525
RR42_RS04530: RR42_RS04530 - pyrimidine permease, at 1,011,333 to 1,012,649
_RS04530
RR42_RS04535: RR42_RS04535 - cysteine synthase, at 1,012,720 to 1,013,622
_RS04535
Position (kb)
1011
1012
1013 Strain fitness (log2 ratio)
-1
0
1
2 at 1010.505 kb on + strand at 1010.505 kb on + strand at 1010.506 kb on - strand at 1010.605 kb on + strand at 1010.606 kb on - strand at 1010.658 kb on - strand at 1010.772 kb on + strand, within RR42_RS04525 at 1011.046 kb on + strand at 1011.047 kb on - strand at 1011.088 kb on + strand at 1011.176 kb on - strand at 1011.296 kb on + strand at 1011.441 kb on - strand at 1011.455 kb on - strand at 1011.579 kb on + strand, within RR42_RS04530 at 1011.579 kb on + strand, within RR42_RS04530 at 1011.580 kb on - strand, within RR42_RS04530 at 1011.580 kb on - strand, within RR42_RS04530 at 1011.699 kb on + strand, within RR42_RS04530 at 1011.700 kb on - strand, within RR42_RS04530 at 1011.700 kb on - strand, within RR42_RS04530 at 1011.700 kb on - strand, within RR42_RS04530 at 1011.700 kb on - strand, within RR42_RS04530 at 1011.848 kb on + strand, within RR42_RS04530 at 1011.929 kb on - strand, within RR42_RS04530 at 1011.964 kb on - strand, within RR42_RS04530 at 1011.964 kb on - strand, within RR42_RS04530 at 1012.173 kb on + strand, within RR42_RS04530 at 1012.174 kb on - strand, within RR42_RS04530 at 1012.174 kb on - strand, within RR42_RS04530 at 1012.251 kb on + strand, within RR42_RS04530 at 1012.251 kb on + strand, within RR42_RS04530 at 1012.252 kb on - strand, within RR42_RS04530 at 1012.252 kb on - strand, within RR42_RS04530 at 1012.252 kb on - strand, within RR42_RS04530 at 1012.442 kb on + strand, within RR42_RS04530 at 1012.686 kb on + strand at 1012.686 kb on + strand at 1012.686 kb on + strand at 1012.687 kb on - strand at 1012.687 kb on - strand at 1012.687 kb on - strand at 1012.727 kb on - strand at 1012.727 kb on - strand at 1012.814 kb on + strand, within RR42_RS04535 at 1012.814 kb on + strand, within RR42_RS04535 at 1012.814 kb on + strand, within RR42_RS04535 at 1012.815 kb on - strand, within RR42_RS04535 at 1012.815 kb on - strand, within RR42_RS04535 at 1012.923 kb on + strand, within RR42_RS04535 at 1013.384 kb on - strand, within RR42_RS04535 at 1013.593 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,010,505 + -0.2 1,010,505 + +1.3 1,010,506 - -0.0 1,010,605 + +0.3 1,010,606 - +0.1 1,010,658 - -1.0 1,010,772 + RR42_RS04525 0.24 -1.5 1,011,046 + -0.0 1,011,047 - -0.3 1,011,088 + -0.2 1,011,176 - +0.8 1,011,296 + -0.5 1,011,441 - +0.5 1,011,455 - +0.6 1,011,579 + RR42_RS04530 0.19 +0.1 1,011,579 + RR42_RS04530 0.19 +0.8 1,011,580 - RR42_RS04530 0.19 +1.2 1,011,580 - RR42_RS04530 0.19 +0.0 1,011,699 + RR42_RS04530 0.28 -0.5 1,011,700 - RR42_RS04530 0.28 +0.7 1,011,700 - RR42_RS04530 0.28 -0.6 1,011,700 - RR42_RS04530 0.28 -0.9 1,011,700 - RR42_RS04530 0.28 -0.9 1,011,848 + RR42_RS04530 0.39 -0.1 1,011,929 - RR42_RS04530 0.45 -0.7 1,011,964 - RR42_RS04530 0.48 +0.6 1,011,964 - RR42_RS04530 0.48 +0.1 1,012,173 + RR42_RS04530 0.64 +0.8 1,012,174 - RR42_RS04530 0.64 -0.6 1,012,174 - RR42_RS04530 0.64 +0.3 1,012,251 + RR42_RS04530 0.70 +0.2 1,012,251 + RR42_RS04530 0.70 -0.9 1,012,252 - RR42_RS04530 0.70 -0.1 1,012,252 - RR42_RS04530 0.70 -0.3 1,012,252 - RR42_RS04530 0.70 +0.2 1,012,442 + RR42_RS04530 0.84 +0.3 1,012,686 + +0.8 1,012,686 + -0.3 1,012,686 + -0.0 1,012,687 - +1.7 1,012,687 - +0.4 1,012,687 - +0.3 1,012,727 - +0.0 1,012,727 - -1.7 1,012,814 + RR42_RS04535 0.10 +2.1 1,012,814 + RR42_RS04535 0.10 -0.7 1,012,814 + RR42_RS04535 0.10 -0.3 1,012,815 - RR42_RS04535 0.11 -0.1 1,012,815 - RR42_RS04535 0.11 -0.4 1,012,923 + RR42_RS04535 0.22 -0.7 1,013,384 - RR42_RS04535 0.74 +0.1 1,013,593 + -0.4
Or see this region's nucleotide sequence