Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04495 and RR42_RS04500 are separated by 206 nucleotides RR42_RS04500 and RR42_RS04505 are separated by 3 nucleotides RR42_RS04505 and RR42_RS04510 are separated by 60 nucleotides
RR42_RS04495: RR42_RS04495 - integration host factor subunit beta, at 1,004,722 to 1,005,219
_RS04495
RR42_RS04500: RR42_RS04500 - membrane protein, at 1,005,426 to 1,005,755
_RS04500
RR42_RS04505: RR42_RS04505 - N-acetylglucosaminyl transferase, at 1,005,759 to 1,006,997
_RS04505
RR42_RS04510: RR42_RS04510 - UDP-glucose 6-dehydrogenase, at 1,007,058 to 1,008,431
_RS04510
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-1
0
1 at 1005.021 kb on + strand, within RR42_RS04495 at 1005.022 kb on - strand, within RR42_RS04495 at 1005.085 kb on - strand, within RR42_RS04495 at 1005.097 kb on - strand, within RR42_RS04495 at 1005.114 kb on + strand, within RR42_RS04495 at 1005.301 kb on + strand at 1005.301 kb on + strand at 1005.302 kb on - strand at 1005.302 kb on - strand at 1005.320 kb on + strand at 1005.729 kb on + strand at 1005.729 kb on + strand at 1005.736 kb on + strand at 1005.736 kb on + strand at 1007.021 kb on + strand at 1007.050 kb on + strand at 1007.057 kb on + strand at 1007.149 kb on + strand at 1007.349 kb on + strand, within RR42_RS04510 at 1007.349 kb on + strand, within RR42_RS04510 at 1007.349 kb on + strand, within RR42_RS04510 at 1007.349 kb on + strand, within RR42_RS04510 at 1007.349 kb on + strand, within RR42_RS04510 at 1007.349 kb on + strand, within RR42_RS04510 at 1007.382 kb on + strand, within RR42_RS04510 at 1007.663 kb on + strand, within RR42_RS04510 at 1007.663 kb on + strand, within RR42_RS04510 at 1007.670 kb on + strand, within RR42_RS04510 at 1007.670 kb on + strand, within RR42_RS04510 at 1007.703 kb on + strand, within RR42_RS04510 at 1007.703 kb on + strand, within RR42_RS04510 at 1007.829 kb on + strand, within RR42_RS04510 at 1007.831 kb on + strand, within RR42_RS04510 at 1007.831 kb on + strand, within RR42_RS04510 at 1007.831 kb on + strand, within RR42_RS04510 at 1007.841 kb on + strand, within RR42_RS04510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,005,021 + RR42_RS04495 0.60 -0.5 1,005,022 - RR42_RS04495 0.60 +0.5 1,005,085 - RR42_RS04495 0.73 +1.1 1,005,097 - RR42_RS04495 0.75 -1.6 1,005,114 + RR42_RS04495 0.79 +0.5 1,005,301 + -0.1 1,005,301 + +0.0 1,005,302 - +0.1 1,005,302 - +0.4 1,005,320 + +0.4 1,005,729 + +1.0 1,005,729 + -0.5 1,005,736 + +0.9 1,005,736 + -0.3 1,007,021 + +0.4 1,007,050 + -1.0 1,007,057 + -0.7 1,007,149 + -1.2 1,007,349 + RR42_RS04510 0.21 -1.7 1,007,349 + RR42_RS04510 0.21 -0.8 1,007,349 + RR42_RS04510 0.21 -0.1 1,007,349 + RR42_RS04510 0.21 -0.5 1,007,349 + RR42_RS04510 0.21 -0.9 1,007,349 + RR42_RS04510 0.21 -0.5 1,007,382 + RR42_RS04510 0.24 -1.2 1,007,663 + RR42_RS04510 0.44 -0.4 1,007,663 + RR42_RS04510 0.44 +0.2 1,007,670 + RR42_RS04510 0.45 +0.4 1,007,670 + RR42_RS04510 0.45 -0.0 1,007,703 + RR42_RS04510 0.47 -0.6 1,007,703 + RR42_RS04510 0.47 +0.1 1,007,829 + RR42_RS04510 0.56 -0.8 1,007,831 + RR42_RS04510 0.56 -0.9 1,007,831 + RR42_RS04510 0.56 +0.1 1,007,831 + RR42_RS04510 0.56 +1.1 1,007,841 + RR42_RS04510 0.57 -1.7
Or see this region's nucleotide sequence