Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04475 and RR42_RS04480 are separated by 0 nucleotides RR42_RS04480 and RR42_RS04485 are separated by 134 nucleotides RR42_RS04485 and RR42_RS04490 are separated by 152 nucleotides
RR42_RS04475: RR42_RS04475 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,000,501 to 1,001,811
_RS04475
RR42_RS04480: RR42_RS04480 - membrane protein, at 1,001,812 to 1,002,045
_RS04480
RR42_RS04485: RR42_RS04485 - cytidylate kinase, at 1,002,180 to 1,002,842
_RS04485
RR42_RS04490: RR42_RS04490 - 30S ribosomal protein S1, at 1,002,995 to 1,004,689
_RS04490
Position (kb) 1001
1002
1003 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1000.828 kb on + strand, within RR42_RS04475 at 1000.829 kb on - strand, within RR42_RS04475 at 1000.851 kb on + strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.852 kb on - strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.898 kb on + strand, within RR42_RS04475 at 1000.915 kb on + strand, within RR42_RS04475 at 1001.104 kb on + strand, within RR42_RS04475 at 1001.248 kb on + strand, within RR42_RS04475 at 1001.249 kb on - strand, within RR42_RS04475 at 1001.347 kb on - strand, within RR42_RS04475 at 1001.432 kb on + strand, within RR42_RS04475 at 1001.451 kb on + strand, within RR42_RS04475 at 1001.506 kb on - strand, within RR42_RS04475 at 1001.615 kb on - strand, within RR42_RS04475 at 1001.677 kb on + strand, within RR42_RS04475 at 1001.678 kb on - strand, within RR42_RS04475 at 1001.779 kb on + strand at 1001.817 kb on + strand at 1001.818 kb on - strand at 1001.912 kb on + strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.913 kb on - strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.987 kb on + strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1001.988 kb on - strand, within RR42_RS04480 at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.048 kb on + strand at 1002.105 kb on + strand at 1002.116 kb on + strand at 1002.117 kb on - strand at 1002.171 kb on + strand at 1002.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,000,828 + RR42_RS04475 0.25 -0.4 1,000,829 - RR42_RS04475 0.25 +0.1 1,000,851 + RR42_RS04475 0.27 -0.6 1,000,852 - RR42_RS04475 0.27 +1.7 1,000,852 - RR42_RS04475 0.27 -0.2 1,000,852 - RR42_RS04475 0.27 -0.5 1,000,852 - RR42_RS04475 0.27 -2.2 1,000,898 + RR42_RS04475 0.30 +0.4 1,000,898 + RR42_RS04475 0.30 -0.1 1,000,915 + RR42_RS04475 0.32 +0.2 1,001,104 + RR42_RS04475 0.46 -0.2 1,001,248 + RR42_RS04475 0.57 -0.1 1,001,249 - RR42_RS04475 0.57 +0.0 1,001,347 - RR42_RS04475 0.65 -0.9 1,001,432 + RR42_RS04475 0.71 -1.1 1,001,451 + RR42_RS04475 0.72 -0.4 1,001,506 - RR42_RS04475 0.77 -0.1 1,001,615 - RR42_RS04475 0.85 +2.4 1,001,677 + RR42_RS04475 0.90 -0.5 1,001,678 - RR42_RS04475 0.90 +1.1 1,001,779 + -1.3 1,001,817 + +0.7 1,001,818 - -0.1 1,001,912 + RR42_RS04480 0.43 -0.9 1,001,913 - RR42_RS04480 0.43 +0.3 1,001,913 - RR42_RS04480 0.43 +0.2 1,001,913 - RR42_RS04480 0.43 +0.9 1,001,913 - RR42_RS04480 0.43 +0.5 1,001,913 - RR42_RS04480 0.43 +0.5 1,001,913 - RR42_RS04480 0.43 +1.5 1,001,987 + RR42_RS04480 0.75 -0.3 1,001,987 + RR42_RS04480 0.75 -0.7 1,001,987 + RR42_RS04480 0.75 +0.6 1,001,987 + RR42_RS04480 0.75 +0.5 1,001,987 + RR42_RS04480 0.75 -0.9 1,001,988 - RR42_RS04480 0.75 +1.1 1,001,988 - RR42_RS04480 0.75 -1.3 1,001,988 - RR42_RS04480 0.75 -1.0 1,001,988 - RR42_RS04480 0.75 +0.5 1,002,048 + -0.6 1,002,048 + -0.1 1,002,048 + +0.4 1,002,048 + -0.1 1,002,048 + -0.1 1,002,105 + -0.9 1,002,116 + +0.0 1,002,117 - -0.5 1,002,171 + -0.5 1,002,185 + +0.9
Or see this region's nucleotide sequence