Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03520 and RR42_RS03525 are separated by 128 nucleotides RR42_RS03525 and RR42_RS03530 are separated by 75 nucleotides RR42_RS03530 and RR42_RS03535 are separated by 6 nucleotides RR42_RS03535 and RR42_RS03540 are separated by 10 nucleotides
RR42_RS03520: RR42_RS03520 - type VI secretion protein EvpB, at 782,557 to 784,050
_RS03520
RR42_RS03525: RR42_RS03525 - type VI secretion system effector, at 784,179 to 784,664
_RS03525
RR42_RS03530: RR42_RS03530 - hypothetical protein, at 784,740 to 784,970
_RS03530
RR42_RS03535: RR42_RS03535 - hypothetical protein, at 784,977 to 785,339
_RS03535
RR42_RS03540: RR42_RS03540 - type VI secretion protein ImpA, at 785,350 to 787,818
_RS03540
Position (kb) 784
785 Strain fitness (log2 ratio) -2
-1
0
1 at 783.844 kb on + strand, within RR42_RS03520 at 783.844 kb on + strand, within RR42_RS03520 at 783.845 kb on - strand, within RR42_RS03520 at 783.845 kb on - strand, within RR42_RS03520 at 783.845 kb on - strand, within RR42_RS03520 at 783.950 kb on - strand at 783.950 kb on - strand at 784.159 kb on + strand at 784.160 kb on - strand at 784.393 kb on - strand, within RR42_RS03525 at 784.465 kb on - strand, within RR42_RS03525 at 784.699 kb on + strand at 784.699 kb on + strand at 784.779 kb on + strand, within RR42_RS03530 at 784.968 kb on + strand at 785.320 kb on - strand at 785.320 kb on - strand at 785.320 kb on - strand at 785.500 kb on + strand at 785.853 kb on - strand, within RR42_RS03540 at 785.944 kb on + strand, within RR42_RS03540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 783,844 + RR42_RS03520 0.86 +1.1 783,844 + RR42_RS03520 0.86 -2.2 783,845 - RR42_RS03520 0.86 +0.6 783,845 - RR42_RS03520 0.86 +1.4 783,845 - RR42_RS03520 0.86 +0.7 783,950 - +0.2 783,950 - +0.8 784,159 + -0.6 784,160 - -1.6 784,393 - RR42_RS03525 0.44 +0.8 784,465 - RR42_RS03525 0.59 +0.8 784,699 + +0.7 784,699 + -0.4 784,779 + RR42_RS03530 0.17 -0.9 784,968 + +1.7 785,320 - +0.6 785,320 - +0.2 785,320 - -0.5 785,500 + -0.3 785,853 - RR42_RS03540 0.20 -0.0 785,944 + RR42_RS03540 0.24 +1.2
Or see this region's nucleotide sequence