Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03280 and RR42_RS03285 are separated by 57 nucleotides RR42_RS03285 and RR42_RS03290 are separated by 146 nucleotides RR42_RS03290 and RR42_RS03295 overlap by 8 nucleotides
RR42_RS03280: RR42_RS03280 - LysR family transcriptional regulator, at 725,760 to 726,665
_RS03280
RR42_RS03285: RR42_RS03285 - tricarboxylic transport membrane protein, at 726,723 to 727,679
_RS03285
RR42_RS03290: RR42_RS03290 - transcriptional regulator, at 727,826 to 728,527
_RS03290
RR42_RS03295: RR42_RS03295 - histidine kinase, at 728,520 to 729,947
_RS03295
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 726.067 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.663 kb on + strand at 726.664 kb on - strand at 726.698 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.788 kb on + strand at 726.788 kb on + strand at 726.898 kb on + strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.928 kb on + strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.166 kb on - strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.320 kb on + strand, within RR42_RS03285 at 727.331 kb on - strand, within RR42_RS03285 at 727.375 kb on + strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.475 kb on - strand, within RR42_RS03285 at 727.505 kb on - strand, within RR42_RS03285 at 727.789 kb on - strand at 727.789 kb on - strand at 727.833 kb on + strand at 727.833 kb on + strand at 727.833 kb on + strand at 727.834 kb on + strand at 727.834 kb on - strand at 727.988 kb on + strand at 727.989 kb on - strand, within RR42_RS03290 at 728.147 kb on + strand, within RR42_RS03290 at 728.147 kb on + strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.148 kb on - strand, within RR42_RS03290 at 728.334 kb on + strand, within RR42_RS03290 at 728.335 kb on - strand, within RR42_RS03290 at 728.335 kb on - strand, within RR42_RS03290 at 728.378 kb on + strand, within RR42_RS03290 at 728.379 kb on - strand, within RR42_RS03290 at 728.432 kb on + strand, within RR42_RS03290 at 728.433 kb on - strand, within RR42_RS03290 at 728.502 kb on - strand at 728.502 kb on - strand at 728.502 kb on - strand at 728.655 kb on + strand at 728.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 726,067 - RR42_RS03280 0.34 +1.5 726,194 - RR42_RS03280 0.48 +1.3 726,194 - RR42_RS03280 0.48 -0.2 726,194 - RR42_RS03280 0.48 -0.5 726,194 - RR42_RS03280 0.48 -0.0 726,663 + +0.5 726,664 - -0.0 726,698 + -0.7 726,784 + +0.2 726,784 + -0.3 726,784 + -0.5 726,784 + -1.0 726,784 + +0.1 726,784 + -0.8 726,784 + +0.7 726,784 + -0.7 726,784 + -0.4 726,784 + +0.4 726,784 + -1.3 726,785 - +0.8 726,785 - +0.9 726,785 - -0.5 726,785 - +0.5 726,785 - +0.6 726,785 - -1.4 726,785 - -0.2 726,785 - -3.0 726,785 - +1.2 726,785 - -1.8 726,788 + +0.2 726,788 + +0.3 726,898 + RR42_RS03285 0.18 -1.4 726,899 - RR42_RS03285 0.18 -0.5 726,899 - RR42_RS03285 0.18 -0.4 726,928 + RR42_RS03285 0.21 -0.3 726,929 - RR42_RS03285 0.22 +0.2 726,929 - RR42_RS03285 0.22 +0.1 727,165 + RR42_RS03285 0.46 -2.3 727,165 + RR42_RS03285 0.46 +0.3 727,166 - RR42_RS03285 0.46 +1.2 727,245 + RR42_RS03285 0.55 +0.5 727,245 + RR42_RS03285 0.55 -0.4 727,245 + RR42_RS03285 0.55 -0.2 727,245 + RR42_RS03285 0.55 +0.6 727,246 - RR42_RS03285 0.55 +0.8 727,246 - RR42_RS03285 0.55 -0.0 727,246 - RR42_RS03285 0.55 +0.0 727,320 + RR42_RS03285 0.62 +1.4 727,331 - RR42_RS03285 0.64 -0.9 727,375 + RR42_RS03285 0.68 -0.5 727,376 - RR42_RS03285 0.68 +0.9 727,376 - RR42_RS03285 0.68 -0.8 727,474 + RR42_RS03285 0.78 -1.5 727,474 + RR42_RS03285 0.78 -0.1 727,474 + RR42_RS03285 0.78 -0.7 727,474 + RR42_RS03285 0.78 +0.1 727,475 - RR42_RS03285 0.79 -0.4 727,505 - RR42_RS03285 0.82 +0.6 727,789 - -0.8 727,789 - -0.2 727,833 + +0.7 727,833 + +0.0 727,833 + +0.1 727,834 + +0.4 727,834 - -1.6 727,988 + +0.2 727,989 - RR42_RS03290 0.23 -0.7 728,147 + RR42_RS03290 0.46 -0.8 728,147 + RR42_RS03290 0.46 -1.1 728,148 - RR42_RS03290 0.46 -1.2 728,148 - RR42_RS03290 0.46 -0.9 728,148 - RR42_RS03290 0.46 +0.6 728,148 - RR42_RS03290 0.46 +0.4 728,334 + RR42_RS03290 0.72 -0.9 728,335 - RR42_RS03290 0.73 -0.2 728,335 - RR42_RS03290 0.73 +2.2 728,378 + RR42_RS03290 0.79 -0.2 728,379 - RR42_RS03290 0.79 -0.2 728,432 + RR42_RS03290 0.86 -1.1 728,433 - RR42_RS03290 0.86 -0.4 728,502 - -0.4 728,502 - +0.9 728,502 - -0.4 728,655 + -0.5 728,656 - -1.4
Or see this region's nucleotide sequence