Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03040 and RR42_RS03045 are separated by 110 nucleotides RR42_RS03045 and RR42_RS03050 are separated by 106 nucleotides
RR42_RS03040: RR42_RS03040 - Outer membrane protein (NodT-like) required for 4-hydroxybenzoate transport, together with FUSC, MFP, and DUF1656 proteins (RR42_RS03025, RR42_RS03035, and RR42_RS03030) (from data) , at 672,626 to 674,089
_RS03040
RR42_RS03045: RR42_RS03045 - dioxygenase, at 674,200 to 675,021
_RS03045
RR42_RS03050: RR42_RS03050 - energy transducer TonB, at 675,128 to 675,868
_RS03050
Position (kb)
674
675
676 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 673.333 kb on - strand, within RR42_RS03040 at 673.436 kb on + strand, within RR42_RS03040 at 673.814 kb on + strand, within RR42_RS03040 at 673.815 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.899 kb on - strand, within RR42_RS03040 at 673.919 kb on + strand, within RR42_RS03040 at 673.920 kb on - strand, within RR42_RS03040 at 674.127 kb on - strand at 674.216 kb on - strand at 674.407 kb on + strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.473 kb on + strand, within RR42_RS03045 at 674.474 kb on - strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.533 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.819 kb on - strand, within RR42_RS03045 at 674.969 kb on + strand at 675.762 kb on + strand, within RR42_RS03050 at 675.762 kb on + strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.846 kb on + strand at 675.847 kb on - strand at 675.847 kb on - strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 673,333 - RR42_RS03040 0.48 +1.0 673,436 + RR42_RS03040 0.55 -0.1 673,814 + RR42_RS03040 0.81 -0.1 673,815 - RR42_RS03040 0.81 +0.2 673,897 - RR42_RS03040 0.87 +0.6 673,897 - RR42_RS03040 0.87 -0.2 673,897 - RR42_RS03040 0.87 -0.2 673,898 + RR42_RS03040 0.87 -1.1 673,898 + RR42_RS03040 0.87 +0.6 673,898 + RR42_RS03040 0.87 +0.3 673,899 - RR42_RS03040 0.87 +0.2 673,919 + RR42_RS03040 0.88 +0.2 673,920 - RR42_RS03040 0.88 -0.8 674,127 - -0.2 674,216 - +0.2 674,407 + RR42_RS03045 0.25 +0.2 674,408 - RR42_RS03045 0.25 -0.5 674,408 - RR42_RS03045 0.25 +0.2 674,408 - RR42_RS03045 0.25 -1.1 674,473 + RR42_RS03045 0.33 +1.2 674,474 - RR42_RS03045 0.33 +0.4 674,530 + RR42_RS03045 0.40 -0.1 674,530 + RR42_RS03045 0.40 +1.2 674,530 + RR42_RS03045 0.40 -0.8 674,530 + RR42_RS03045 0.40 -1.6 674,530 + RR42_RS03045 0.40 +0.9 674,531 - RR42_RS03045 0.40 +0.4 674,531 - RR42_RS03045 0.40 +0.0 674,531 - RR42_RS03045 0.40 -1.0 674,531 - RR42_RS03045 0.40 -0.2 674,531 - RR42_RS03045 0.40 -1.0 674,531 - RR42_RS03045 0.40 -0.3 674,531 - RR42_RS03045 0.40 +0.6 674,531 - RR42_RS03045 0.40 +2.3 674,532 + RR42_RS03045 0.40 -0.5 674,532 + RR42_RS03045 0.40 +0.5 674,532 + RR42_RS03045 0.40 -1.1 674,532 + RR42_RS03045 0.40 -1.0 674,532 + RR42_RS03045 0.40 -0.6 674,533 - RR42_RS03045 0.41 +0.6 674,552 - RR42_RS03045 0.43 -2.0 674,552 - RR42_RS03045 0.43 -0.0 674,819 - RR42_RS03045 0.75 -0.4 674,969 + +0.2 675,762 + RR42_RS03050 0.86 -0.4 675,762 + RR42_RS03050 0.86 +0.3 675,766 - RR42_RS03050 0.86 +0.9 675,766 - RR42_RS03050 0.86 +0.4 675,766 - RR42_RS03050 0.86 +0.3 675,846 + -0.6 675,847 - +0.0 675,847 - +0.2 675,900 + -0.5 675,900 + +0.6 675,900 + +0.8 675,900 + -2.4 675,900 + -0.3 675,901 - +0.1 675,901 - +2.2 675,901 - +0.2 675,901 - -0.6 676,004 + +0.3 676,004 + +0.9 676,004 + -0.9 676,004 + +0.7 676,005 - -0.6
Or see this region's nucleotide sequence