Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02855 and RR42_RS02860 are separated by 94 nucleotides RR42_RS02860 and RR42_RS02865 are separated by 123 nucleotides
RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543
_RS02855
RR42_RS02860: RR42_RS02860 - hypothetical protein, at 637,638 to 638,018
_RS02860
RR42_RS02865: RR42_RS02865 - chloride channel protein, at 638,142 to 639,830
_RS02865
Position (kb)
637
638
639 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 636.688 kb on - strand, within RR42_RS02855 at 636.789 kb on + strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.838 kb on - strand, within RR42_RS02855 at 636.931 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.475 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.634 kb on - strand at 637.634 kb on - strand at 637.639 kb on + strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.763 kb on + strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 638.106 kb on + strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.143 kb on + strand at 638.144 kb on - strand at 638.144 kb on - strand at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.414 kb on - strand, within RR42_RS02865 at 638.419 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.575 kb on + strand, within RR42_RS02865 at 638.576 kb on - strand, within RR42_RS02865 at 638.608 kb on + strand, within RR42_RS02865 at 638.609 kb on - strand, within RR42_RS02865 at 638.612 kb on - strand, within RR42_RS02865 at 638.902 kb on - strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.941 kb on + strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865 at 638.942 kb on - strand, within RR42_RS02865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 636,688 - RR42_RS02855 0.37 +0.2 636,789 + RR42_RS02855 0.44 +0.2 636,790 - RR42_RS02855 0.44 -0.6 636,790 - RR42_RS02855 0.44 -0.6 636,790 - RR42_RS02855 0.44 -0.1 636,838 - RR42_RS02855 0.48 -1.1 636,931 - RR42_RS02855 0.55 +0.7 637,005 - RR42_RS02855 0.60 +1.4 637,005 - RR42_RS02855 0.60 -2.4 637,060 - RR42_RS02855 0.64 +0.6 637,060 - RR42_RS02855 0.64 -0.1 637,069 - RR42_RS02855 0.65 +0.9 637,069 - RR42_RS02855 0.65 -1.0 637,475 - -1.7 637,519 - -0.3 637,519 - -0.2 637,519 - -0.2 637,634 - -0.3 637,634 - +0.2 637,639 + -0.6 637,640 - +0.3 637,640 - +2.2 637,640 - -0.7 637,640 - -1.1 637,763 + RR42_RS02860 0.33 +0.2 637,960 - RR42_RS02860 0.85 -0.4 637,960 - RR42_RS02860 0.85 -1.2 638,106 + +0.6 638,107 - +1.8 638,107 - +0.7 638,107 - +0.2 638,107 - +0.4 638,110 - -0.5 638,110 - -0.5 638,110 - +1.5 638,110 - -0.3 638,110 - +0.1 638,143 + -0.2 638,144 - +0.9 638,144 - +0.5 638,413 + RR42_RS02865 0.16 -0.4 638,413 + RR42_RS02865 0.16 -0.1 638,413 + RR42_RS02865 0.16 -0.2 638,413 + RR42_RS02865 0.16 +0.5 638,414 - RR42_RS02865 0.16 -0.1 638,419 + RR42_RS02865 0.16 -0.5 638,575 + RR42_RS02865 0.26 -1.5 638,575 + RR42_RS02865 0.26 +0.0 638,575 + RR42_RS02865 0.26 +0.1 638,576 - RR42_RS02865 0.26 -0.0 638,608 + RR42_RS02865 0.28 +0.6 638,609 - RR42_RS02865 0.28 +0.6 638,612 - RR42_RS02865 0.28 +0.2 638,902 - RR42_RS02865 0.45 -2.3 638,941 + RR42_RS02865 0.47 -0.0 638,941 + RR42_RS02865 0.47 +0.2 638,941 + RR42_RS02865 0.47 +0.2 638,941 + RR42_RS02865 0.47 +0.6 638,942 - RR42_RS02865 0.47 -0.8 638,942 - RR42_RS02865 0.47 +1.2 638,942 - RR42_RS02865 0.47 -0.2 638,942 - RR42_RS02865 0.47 -1.1 638,942 - RR42_RS02865 0.47 +0.6 638,942 - RR42_RS02865 0.47 +1.1
Or see this region's nucleotide sequence