Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02810 and RR42_RS02815 are separated by 17 nucleotides RR42_RS02815 and RR42_RS02820 are separated by 29 nucleotides RR42_RS02820 and RR42_RS02825 are separated by 36 nucleotides RR42_RS02825 and RR42_RS02830 overlap by 4 nucleotides
RR42_RS02810: RR42_RS02810 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 625,577 to 626,329
_RS02810
RR42_RS02815: RR42_RS02815 - D,D-heptose 1,7-bisphosphate phosphatase, at 626,347 to 626,925
_RS02815
RR42_RS02820: RR42_RS02820 - glycyl-tRNA synthetase subunit beta, at 626,955 to 629,087
_RS02820
RR42_RS02825: RR42_RS02825 - hypothetical protein, at 629,124 to 629,780
_RS02825
RR42_RS02830: RR42_RS02830 - glycyl-tRNA synthetase subunit alpha, at 629,777 to 630,727
_RS02830
Position (kb) 626
627
628
629
630 Strain fitness (log2 ratio) -1
0
1 at 626.061 kb on + strand, within RR42_RS02810 at 626.956 kb on + strand at 629.144 kb on + strand at 629.144 kb on + strand at 629.145 kb on - strand at 629.145 kb on - strand at 629.145 kb on - strand at 629.390 kb on - strand, within RR42_RS02825 at 629.390 kb on - strand, within RR42_RS02825 at 629.390 kb on - strand, within RR42_RS02825 at 629.393 kb on - strand, within RR42_RS02825 at 629.393 kb on - strand, within RR42_RS02825 at 629.666 kb on - strand, within RR42_RS02825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 626,061 + RR42_RS02810 0.64 -0.6 626,956 + -1.7 629,144 + -1.5 629,144 + +1.1 629,145 - -0.6 629,145 - -0.2 629,145 - +0.5 629,390 - RR42_RS02825 0.40 -0.1 629,390 - RR42_RS02825 0.40 +0.1 629,390 - RR42_RS02825 0.40 +1.1 629,393 - RR42_RS02825 0.41 -1.2 629,393 - RR42_RS02825 0.41 +0.6 629,666 - RR42_RS02825 0.82 +0.4
Or see this region's nucleotide sequence