Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02800 and RR42_RS02805 are separated by 116 nucleotides RR42_RS02805 and RR42_RS02810 overlap by 4 nucleotides RR42_RS02810 and RR42_RS02815 are separated by 17 nucleotides RR42_RS02815 and RR42_RS02820 are separated by 29 nucleotides
RR42_RS02800: RR42_RS02800 - glyoxalase I, at 624,172 to 624,579
_RS02800
RR42_RS02805: RR42_RS02805 - metal-dependent hydrolase, at 624,696 to 625,580
_RS02805
RR42_RS02810: RR42_RS02810 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 625,577 to 626,329
_RS02810
RR42_RS02815: RR42_RS02815 - D,D-heptose 1,7-bisphosphate phosphatase, at 626,347 to 626,925
_RS02815
RR42_RS02820: RR42_RS02820 - glycyl-tRNA synthetase subunit beta, at 626,955 to 629,087
_RS02820
Position (kb) 625
626
627 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 624.597 kb on - strand at 624.619 kb on - strand at 624.641 kb on + strand at 624.641 kb on + strand at 624.642 kb on - strand at 624.698 kb on - strand at 624.951 kb on - strand, within RR42_RS02805 at 624.976 kb on + strand, within RR42_RS02805 at 624.976 kb on + strand, within RR42_RS02805 at 624.977 kb on - strand, within RR42_RS02805 at 624.980 kb on + strand, within RR42_RS02805 at 624.981 kb on - strand, within RR42_RS02805 at 624.981 kb on - strand, within RR42_RS02805 at 625.375 kb on + strand, within RR42_RS02805 at 625.781 kb on + strand, within RR42_RS02810 at 625.782 kb on - strand, within RR42_RS02810 at 625.828 kb on - strand, within RR42_RS02810 at 626.061 kb on + strand, within RR42_RS02810 at 626.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 624,597 - -0.2 624,619 - +0.1 624,641 + -0.3 624,641 + -0.7 624,642 - -0.2 624,698 - +1.0 624,951 - RR42_RS02805 0.29 -2.0 624,976 + RR42_RS02805 0.32 +0.6 624,976 + RR42_RS02805 0.32 -0.2 624,977 - RR42_RS02805 0.32 -1.2 624,980 + RR42_RS02805 0.32 -0.1 624,981 - RR42_RS02805 0.32 -1.8 624,981 - RR42_RS02805 0.32 +0.9 625,375 + RR42_RS02805 0.77 -0.0 625,781 + RR42_RS02810 0.27 -2.8 625,782 - RR42_RS02810 0.27 -1.6 625,828 - RR42_RS02810 0.33 -1.6 626,061 + RR42_RS02810 0.64 -0.6 626,956 + -1.7
Or see this region's nucleotide sequence