Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02185 and RR42_RS02190 overlap by 4 nucleotides RR42_RS02190 and RR42_RS02195 are separated by 2 nucleotides RR42_RS02195 and RR42_RS02200 are separated by 41 nucleotides
RR42_RS02185: RR42_RS02185 - fumarate reductase, at 485,877 to 487,604
_RS02185
RR42_RS02190: RR42_RS02190 - fumarate hydrolyase, at 487,601 to 488,215
_RS02190
RR42_RS02195: RR42_RS02195 - fumarate hydratase, at 488,218 to 489,093
_RS02195
RR42_RS02200: RR42_RS02200 - LacI family transcriptional regulator, at 489,135 to 490,130
_RS02200
Position (kb) 488
489
490 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 487.675 kb on - strand, within RR42_RS02190 at 487.675 kb on - strand, within RR42_RS02190 at 487.791 kb on - strand, within RR42_RS02190 at 487.837 kb on - strand, within RR42_RS02190 at 487.907 kb on + strand, within RR42_RS02190 at 487.907 kb on + strand, within RR42_RS02190 at 487.908 kb on - strand, within RR42_RS02190 at 488.095 kb on + strand, within RR42_RS02190 at 488.095 kb on + strand, within RR42_RS02190 at 488.096 kb on - strand, within RR42_RS02190 at 488.096 kb on - strand, within RR42_RS02190 at 488.131 kb on + strand, within RR42_RS02190 at 488.206 kb on - strand at 488.206 kb on - strand at 488.206 kb on - strand at 488.314 kb on - strand, within RR42_RS02195 at 488.332 kb on + strand, within RR42_RS02195 at 488.335 kb on - strand, within RR42_RS02195 at 488.818 kb on + strand, within RR42_RS02195 at 488.819 kb on - strand, within RR42_RS02195 at 488.876 kb on + strand, within RR42_RS02195 at 488.877 kb on - strand, within RR42_RS02195 at 489.047 kb on + strand at 489.048 kb on - strand at 489.207 kb on + strand at 489.208 kb on - strand at 489.560 kb on - strand, within RR42_RS02200 at 489.560 kb on - strand, within RR42_RS02200 at 489.759 kb on - strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 487,675 - RR42_RS02190 0.12 -0.5 487,675 - RR42_RS02190 0.12 -1.2 487,791 - RR42_RS02190 0.31 +0.7 487,837 - RR42_RS02190 0.38 -1.0 487,907 + RR42_RS02190 0.50 +0.2 487,907 + RR42_RS02190 0.50 -0.5 487,908 - RR42_RS02190 0.50 -0.7 488,095 + RR42_RS02190 0.80 -2.2 488,095 + RR42_RS02190 0.80 -0.8 488,096 - RR42_RS02190 0.80 +0.8 488,096 - RR42_RS02190 0.80 +0.8 488,131 + RR42_RS02190 0.86 +0.9 488,206 - +0.9 488,206 - +0.9 488,206 - +1.2 488,314 - RR42_RS02195 0.11 -0.6 488,332 + RR42_RS02195 0.13 +0.4 488,335 - RR42_RS02195 0.13 -0.4 488,818 + RR42_RS02195 0.68 +0.7 488,819 - RR42_RS02195 0.69 +0.2 488,876 + RR42_RS02195 0.75 -0.7 488,877 - RR42_RS02195 0.75 -0.6 489,047 + -3.4 489,048 - -0.4 489,207 + +0.7 489,208 - -1.1 489,560 - RR42_RS02200 0.43 -0.0 489,560 - RR42_RS02200 0.43 -0.4 489,759 - RR42_RS02200 0.63 -0.1 489,845 + RR42_RS02200 0.71 +0.4 489,845 + RR42_RS02200 0.71 -0.2 489,845 + RR42_RS02200 0.71 +0.4 489,846 - RR42_RS02200 0.71 -0.1 489,846 - RR42_RS02200 0.71 +0.1 489,848 - RR42_RS02200 0.72 +1.7 489,848 - RR42_RS02200 0.72 -0.4
Or see this region's nucleotide sequence