Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02145 and RR42_RS02150 are separated by 393 nucleotides RR42_RS02150 and RR42_RS02155 are separated by 197 nucleotides RR42_RS02155 and RR42_RS02160 are separated by 128 nucleotides
RR42_RS02145: RR42_RS02145 - excinuclease ABC subunit A, at 474,837 to 477,701
_RS02145
RR42_RS02150: RR42_RS02150 - membrane protein, at 478,095 to 479,351
_RS02150
RR42_RS02155: RR42_RS02155 - single-stranded DNA-binding protein, at 479,549 to 480,103
_RS02155
RR42_RS02160: RR42_RS02160 - LacI family transcriptional regulator, at 480,232 to 481,215
_RS02160
Position (kb) 478
479
480 Strain fitness (log2 ratio) -2
-1
0
1
2 at 477.121 kb on + strand, within RR42_RS02145 at 477.121 kb on + strand, within RR42_RS02145 at 477.121 kb on + strand, within RR42_RS02145 at 477.346 kb on + strand, within RR42_RS02145 at 477.382 kb on + strand, within RR42_RS02145 at 477.383 kb on - strand, within RR42_RS02145 at 477.524 kb on - strand at 477.545 kb on - strand at 477.545 kb on - strand at 477.701 kb on + strand at 477.701 kb on + strand at 477.701 kb on + strand at 477.701 kb on + strand at 477.701 kb on + strand at 477.702 kb on - strand at 477.702 kb on - strand at 477.702 kb on - strand at 477.702 kb on - strand at 477.703 kb on + strand at 477.703 kb on + strand at 477.703 kb on + strand at 477.703 kb on + strand at 477.704 kb on - strand at 477.704 kb on - strand at 477.901 kb on + strand at 477.902 kb on - strand at 477.902 kb on - strand at 477.904 kb on + strand at 477.904 kb on + strand at 477.905 kb on - strand at 477.942 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.109 kb on + strand at 478.110 kb on - strand at 478.110 kb on - strand at 478.903 kb on - strand, within RR42_RS02150 at 478.903 kb on - strand, within RR42_RS02150 at 479.167 kb on - strand, within RR42_RS02150 at 479.167 kb on - strand, within RR42_RS02150 at 479.285 kb on + strand at 479.285 kb on + strand at 479.287 kb on + strand at 479.417 kb on + strand at 479.421 kb on + strand at 479.421 kb on + strand at 479.468 kb on + strand at 480.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 477,121 + RR42_RS02145 0.80 +0.1 477,121 + RR42_RS02145 0.80 -0.2 477,121 + RR42_RS02145 0.80 +0.5 477,346 + RR42_RS02145 0.88 +0.7 477,382 + RR42_RS02145 0.89 +0.2 477,383 - RR42_RS02145 0.89 -1.0 477,524 - -0.1 477,545 - +0.4 477,545 - -0.6 477,701 + -0.0 477,701 + -0.6 477,701 + +0.1 477,701 + -0.1 477,701 + +0.2 477,702 - +0.8 477,702 - -0.1 477,702 - -0.7 477,702 - -1.3 477,703 + +0.7 477,703 + +0.8 477,703 + +0.2 477,703 + +0.3 477,704 - +0.4 477,704 - +0.4 477,901 + -1.6 477,902 - +0.2 477,902 - +0.1 477,904 + -0.2 477,904 + +0.4 477,905 - -1.6 477,942 + +0.6 478,109 + -1.5 478,109 + -2.1 478,109 + -0.2 478,109 + +0.6 478,109 + -2.3 478,109 + -1.4 478,110 - +0.1 478,110 - -0.8 478,903 - RR42_RS02150 0.64 -1.8 478,903 - RR42_RS02150 0.64 -0.7 479,167 - RR42_RS02150 0.85 -0.2 479,167 - RR42_RS02150 0.85 +1.2 479,285 + -1.8 479,285 + -1.2 479,287 + +0.9 479,417 + -0.2 479,421 + +0.4 479,421 + +2.0 479,468 + +0.9 480,321 + +1.8
Or see this region's nucleotide sequence