Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02085 and RR42_RS02090 are separated by 138 nucleotides RR42_RS02090 and RR42_RS02095 are separated by 24 nucleotides RR42_RS02095 and RR42_RS02100 are separated by 54 nucleotides RR42_RS02100 and RR42_RS02105 are separated by 15 nucleotides
RR42_RS02085: RR42_RS02085 - RNA polymerase subunit sigma-54, at 463,217 to 464,692
_RS02085
RR42_RS02090: RR42_RS02090 - sugar ABC transporter ATP-binding protein, at 464,831 to 465,616
_RS02090
RR42_RS02095: RR42_RS02095 - organic solvent tolerance protein OstA, at 465,641 to 466,285
_RS02095
RR42_RS02100: RR42_RS02100 - hypothetical protein, at 466,340 to 466,966
_RS02100
RR42_RS02105: RR42_RS02105 - arabinose 5-phosphate isomerase, at 466,982 to 467,965
_RS02105
Position (kb) 465
466
467 Strain fitness (log2 ratio) -2
-1
0
1 at 464.647 kb on + strand at 464.679 kb on - strand at 464.679 kb on - strand at 464.699 kb on + strand at 464.704 kb on + strand at 464.704 kb on + strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.741 kb on - strand at 464.773 kb on - strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.833 kb on - strand at 464.833 kb on - strand at 466.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 464,647 + -0.2 464,679 - +1.0 464,679 - -0.6 464,699 + +1.2 464,704 + +1.6 464,704 + -1.8 464,705 - +1.6 464,705 - -1.9 464,705 - -0.3 464,705 - -0.9 464,741 - +0.6 464,773 - -0.6 464,832 + +0.0 464,832 + -0.9 464,832 + -0.8 464,832 + +0.4 464,832 + +0.2 464,832 + -1.3 464,833 - -0.0 464,833 - -0.4 466,389 - +0.4
Or see this region's nucleotide sequence