Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02075 and RR42_RS02080 are separated by 384 nucleotides RR42_RS02080 and RR42_RS02085 are separated by 49 nucleotides RR42_RS02085 and RR42_RS02090 are separated by 138 nucleotides RR42_RS02090 and RR42_RS02095 are separated by 24 nucleotides
RR42_RS02075: RR42_RS02075 - PTS sugar transporter subunit IIA, at 461,977 to 462,432
_RS02075
RR42_RS02080: RR42_RS02080 - ribosome hibernation promoting factor, at 462,817 to 463,167
_RS02080
RR42_RS02085: RR42_RS02085 - RNA polymerase subunit sigma-54, at 463,217 to 464,692
_RS02085
RR42_RS02090: RR42_RS02090 - sugar ABC transporter ATP-binding protein, at 464,831 to 465,616
_RS02090
RR42_RS02095: RR42_RS02095 - organic solvent tolerance protein OstA, at 465,641 to 466,285
_RS02095
Position (kb) 463
464
465 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 462.454 kb on - strand at 462.454 kb on - strand at 462.568 kb on - strand at 462.568 kb on - strand at 462.569 kb on + strand at 462.569 kb on + strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.570 kb on - strand at 462.603 kb on + strand at 462.604 kb on - strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.657 kb on + strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.658 kb on - strand at 462.661 kb on + strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.662 kb on - strand at 462.673 kb on + strand at 462.673 kb on + strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.674 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.682 kb on - strand at 462.685 kb on + strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.686 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.722 kb on - strand at 462.797 kb on - strand at 462.849 kb on - strand at 462.849 kb on - strand at 462.942 kb on - strand, within RR42_RS02080 at 462.942 kb on - strand, within RR42_RS02080 at 462.991 kb on + strand, within RR42_RS02080 at 462.991 kb on + strand, within RR42_RS02080 at 462.992 kb on - strand, within RR42_RS02080 at 463.032 kb on + strand, within RR42_RS02080 at 463.032 kb on + strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.033 kb on - strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.057 kb on + strand, within RR42_RS02080 at 463.089 kb on + strand, within RR42_RS02080 at 463.112 kb on + strand, within RR42_RS02080 at 463.112 kb on + strand, within RR42_RS02080 at 463.113 kb on - strand, within RR42_RS02080 at 463.113 kb on - strand, within RR42_RS02080 at 463.168 kb on - strand at 463.218 kb on + strand at 463.218 kb on + strand at 463.218 kb on + strand at 463.219 kb on - strand at 463.229 kb on - strand at 463.238 kb on + strand at 463.428 kb on - strand, within RR42_RS02085 at 463.482 kb on + strand, within RR42_RS02085 at 463.483 kb on - strand, within RR42_RS02085 at 463.486 kb on + strand, within RR42_RS02085 at 463.487 kb on - strand, within RR42_RS02085 at 463.531 kb on + strand, within RR42_RS02085 at 463.588 kb on - strand, within RR42_RS02085 at 463.628 kb on + strand, within RR42_RS02085 at 463.629 kb on - strand, within RR42_RS02085 at 463.651 kb on + strand, within RR42_RS02085 at 463.652 kb on - strand, within RR42_RS02085 at 463.740 kb on + strand, within RR42_RS02085 at 463.741 kb on - strand, within RR42_RS02085 at 463.741 kb on - strand, within RR42_RS02085 at 463.857 kb on + strand, within RR42_RS02085 at 464.188 kb on - strand, within RR42_RS02085 at 464.221 kb on + strand, within RR42_RS02085 at 464.403 kb on + strand, within RR42_RS02085 at 464.404 kb on - strand, within RR42_RS02085 at 464.647 kb on + strand at 464.679 kb on - strand at 464.679 kb on - strand at 464.699 kb on + strand at 464.704 kb on + strand at 464.704 kb on + strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.705 kb on - strand at 464.741 kb on - strand at 464.773 kb on - strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.832 kb on + strand at 464.833 kb on - strand at 464.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 462,454 - +1.2 462,454 - +0.6 462,568 - +0.3 462,568 - +0.4 462,569 + +0.0 462,569 + +2.8 462,570 - -1.6 462,570 - -2.1 462,570 - -1.8 462,570 - -0.6 462,570 - -0.7 462,570 - -0.4 462,570 - +0.2 462,570 - +0.9 462,570 - -0.8 462,603 + +0.0 462,604 - -1.6 462,657 + +1.2 462,657 + -0.8 462,657 + +0.0 462,657 + +1.2 462,657 + -0.8 462,658 - +1.2 462,658 - +0.6 462,658 - -0.8 462,658 - -1.9 462,658 - -0.6 462,661 + +1.3 462,662 - +0.4 462,662 - +0.4 462,662 - -0.8 462,662 - -1.1 462,662 - -1.2 462,673 + +0.6 462,673 + -0.1 462,674 - -1.2 462,674 - -1.2 462,674 - +0.4 462,682 - -1.5 462,682 - -0.9 462,682 - -0.5 462,682 - +0.2 462,682 - -0.1 462,685 + -1.8 462,686 - +0.2 462,686 - -0.8 462,686 - -0.4 462,686 - +0.3 462,722 - -0.7 462,722 - -2.4 462,722 - -0.5 462,797 - -0.5 462,849 - -0.1 462,849 - +0.3 462,942 - RR42_RS02080 0.36 +0.2 462,942 - RR42_RS02080 0.36 -0.5 462,991 + RR42_RS02080 0.50 -0.4 462,991 + RR42_RS02080 0.50 +0.0 462,992 - RR42_RS02080 0.50 +1.2 463,032 + RR42_RS02080 0.61 -0.2 463,032 + RR42_RS02080 0.61 -0.2 463,033 - RR42_RS02080 0.62 +0.0 463,033 - RR42_RS02080 0.62 +0.2 463,033 - RR42_RS02080 0.62 -0.5 463,057 + RR42_RS02080 0.68 -0.8 463,057 + RR42_RS02080 0.68 +0.9 463,057 + RR42_RS02080 0.68 -1.1 463,089 + RR42_RS02080 0.77 -0.1 463,112 + RR42_RS02080 0.84 +1.5 463,112 + RR42_RS02080 0.84 -0.4 463,113 - RR42_RS02080 0.84 -0.4 463,113 - RR42_RS02080 0.84 -0.8 463,168 - -0.5 463,218 + -0.4 463,218 + -0.9 463,218 + -0.5 463,219 - +0.2 463,229 - -0.4 463,238 + +1.2 463,428 - RR42_RS02085 0.14 +0.8 463,482 + RR42_RS02085 0.18 +0.2 463,483 - RR42_RS02085 0.18 +1.9 463,486 + RR42_RS02085 0.18 +2.8 463,487 - RR42_RS02085 0.18 +2.0 463,531 + RR42_RS02085 0.21 +0.9 463,588 - RR42_RS02085 0.25 +1.5 463,628 + RR42_RS02085 0.28 -0.4 463,629 - RR42_RS02085 0.28 +0.1 463,651 + RR42_RS02085 0.29 +0.6 463,652 - RR42_RS02085 0.29 -0.4 463,740 + RR42_RS02085 0.35 +0.4 463,741 - RR42_RS02085 0.36 +0.3 463,741 - RR42_RS02085 0.36 +0.6 463,857 + RR42_RS02085 0.43 +1.0 464,188 - RR42_RS02085 0.66 +1.2 464,221 + RR42_RS02085 0.68 +0.2 464,403 + RR42_RS02085 0.80 +1.2 464,404 - RR42_RS02085 0.80 +1.1 464,647 + -0.2 464,679 - +1.0 464,679 - -0.6 464,699 + +1.2 464,704 + +1.6 464,704 + -1.8 464,705 - +1.6 464,705 - -1.9 464,705 - -0.3 464,705 - -0.9 464,741 - +0.6 464,773 - -0.6 464,832 + +0.0 464,832 + -0.9 464,832 + -0.8 464,832 + +0.4 464,832 + +0.2 464,832 + -1.3 464,833 - -0.0 464,833 - -0.4
Or see this region's nucleotide sequence