Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02040 and RR42_RS02045 are separated by 283 nucleotides RR42_RS02045 and RR42_RS02050 are separated by 99 nucleotides
RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171
_RS02040
RR42_RS02045: RR42_RS02045 - GTPase, at 455,455 to 457,398
_RS02045
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
Position (kb)
455
456
457
458 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 454.464 kb on + strand, within RR42_RS02040 at 454.464 kb on + strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.465 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.792 kb on - strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.868 kb on + strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.869 kb on - strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.872 kb on + strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 454.873 kb on - strand, within RR42_RS02040 at 455.071 kb on - strand, within RR42_RS02040 at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.148 kb on + strand at 455.214 kb on - strand at 455.232 kb on + strand at 455.232 kb on + strand at 455.233 kb on - strand at 455.327 kb on - strand at 455.327 kb on - strand at 455.351 kb on + strand at 455.352 kb on - strand at 455.485 kb on + strand at 455.485 kb on + strand at 455.486 kb on - strand at 455.508 kb on - strand at 455.508 kb on - strand at 455.557 kb on + strand at 455.590 kb on - strand at 455.727 kb on + strand at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.727 kb on + strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.728 kb on - strand, within RR42_RS02045 at 455.753 kb on - strand, within RR42_RS02045 at 455.853 kb on + strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.854 kb on - strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.959 kb on + strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 455.960 kb on - strand, within RR42_RS02045 at 456.376 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 457.059 kb on + strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.107 kb on + strand, within RR42_RS02045 at 457.295 kb on + strand at 457.295 kb on + strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.298 kb on + strand at 457.298 kb on + strand at 457.299 kb on - strand at 457.492 kb on + strand at 457.642 kb on + strand, within RR42_RS02050 at 457.702 kb on + strand, within RR42_RS02050 at 457.703 kb on - strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.798 kb on + strand, within RR42_RS02050 at 457.799 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 454,464 + RR42_RS02040 0.20 -0.2 454,464 + RR42_RS02040 0.20 -0.8 454,465 - RR42_RS02040 0.20 +1.5 454,465 - RR42_RS02040 0.20 +0.9 454,792 - RR42_RS02040 0.57 +1.8 454,792 - RR42_RS02040 0.57 -0.1 454,868 + RR42_RS02040 0.66 -0.7 454,868 + RR42_RS02040 0.66 -0.6 454,868 + RR42_RS02040 0.66 +0.9 454,868 + RR42_RS02040 0.66 +0.1 454,868 + RR42_RS02040 0.66 -0.1 454,869 - RR42_RS02040 0.66 -0.6 454,869 - RR42_RS02040 0.66 -1.1 454,869 - RR42_RS02040 0.66 -1.1 454,869 - RR42_RS02040 0.66 +2.2 454,869 - RR42_RS02040 0.66 -0.0 454,869 - RR42_RS02040 0.66 -1.1 454,869 - RR42_RS02040 0.66 +1.2 454,869 - RR42_RS02040 0.66 +0.7 454,872 + RR42_RS02040 0.66 -0.4 454,872 + RR42_RS02040 0.66 +0.9 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -0.6 454,872 + RR42_RS02040 0.66 +0.4 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 -1.6 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -0.2 454,872 + RR42_RS02040 0.66 -0.5 454,872 + RR42_RS02040 0.66 -0.4 454,873 - RR42_RS02040 0.66 +0.8 454,873 - RR42_RS02040 0.66 +0.3 454,873 - RR42_RS02040 0.66 -1.6 454,873 - RR42_RS02040 0.66 +0.1 454,873 - RR42_RS02040 0.66 -0.7 454,873 - RR42_RS02040 0.66 +0.9 454,873 - RR42_RS02040 0.66 +0.7 454,873 - RR42_RS02040 0.66 -0.1 454,873 - RR42_RS02040 0.66 +0.1 455,071 - RR42_RS02040 0.89 -0.4 455,148 + +0.4 455,148 + +0.5 455,148 + -0.1 455,148 + -0.2 455,214 - -1.6 455,232 + +0.6 455,232 + -0.4 455,233 - +1.0 455,327 - -0.9 455,327 - +0.8 455,351 + -0.4 455,352 - +0.4 455,485 + +1.1 455,485 + +2.8 455,486 - +1.2 455,508 - +1.4 455,508 - +2.5 455,557 + +2.2 455,590 - -1.8 455,727 + +0.7 455,727 + RR42_RS02045 0.14 +2.0 455,727 + RR42_RS02045 0.14 +1.2 455,727 + RR42_RS02045 0.14 +0.8 455,727 + RR42_RS02045 0.14 +1.7 455,728 - RR42_RS02045 0.14 +1.1 455,728 - RR42_RS02045 0.14 +2.4 455,728 - RR42_RS02045 0.14 +1.9 455,728 - RR42_RS02045 0.14 +0.8 455,728 - RR42_RS02045 0.14 +1.2 455,753 - RR42_RS02045 0.15 +1.7 455,853 + RR42_RS02045 0.20 +1.8 455,854 - RR42_RS02045 0.21 +1.6 455,854 - RR42_RS02045 0.21 +1.7 455,959 + RR42_RS02045 0.26 +0.7 455,959 + RR42_RS02045 0.26 +1.1 455,960 - RR42_RS02045 0.26 +1.3 455,960 - RR42_RS02045 0.26 +0.7 456,376 + RR42_RS02045 0.47 +0.1 456,928 + RR42_RS02045 0.76 +1.8 456,928 + RR42_RS02045 0.76 +1.0 456,929 - RR42_RS02045 0.76 +0.8 456,929 - RR42_RS02045 0.76 +0.9 456,929 - RR42_RS02045 0.76 +1.9 456,957 + RR42_RS02045 0.77 +0.9 456,957 + RR42_RS02045 0.77 +1.3 456,957 + RR42_RS02045 0.77 +1.3 456,957 + RR42_RS02045 0.77 +1.1 456,958 - RR42_RS02045 0.77 -0.1 456,958 - RR42_RS02045 0.77 +1.6 456,958 - RR42_RS02045 0.77 +1.0 456,958 - RR42_RS02045 0.77 +0.6 456,958 - RR42_RS02045 0.77 +0.2 456,994 + RR42_RS02045 0.79 +0.8 456,994 + RR42_RS02045 0.79 +1.2 456,994 + RR42_RS02045 0.79 +1.9 456,994 + RR42_RS02045 0.79 +1.2 456,995 - RR42_RS02045 0.79 +1.6 456,995 - RR42_RS02045 0.79 +1.8 457,059 + RR42_RS02045 0.83 +1.5 457,060 - RR42_RS02045 0.83 +0.8 457,060 - RR42_RS02045 0.83 +2.2 457,107 + RR42_RS02045 0.85 +0.6 457,295 + +0.9 457,295 + +0.8 457,296 - +1.8 457,296 - +1.3 457,296 - -0.5 457,298 + +0.7 457,298 + +0.6 457,299 - +1.2 457,492 + -0.3 457,642 + RR42_RS02050 0.12 -0.4 457,702 + RR42_RS02050 0.17 -0.8 457,703 - RR42_RS02050 0.18 +0.0 457,795 + RR42_RS02050 0.25 -1.5 457,795 + RR42_RS02050 0.25 -0.0 457,798 + RR42_RS02050 0.26 +1.0 457,799 - RR42_RS02050 0.26 +0.6 458,108 - RR42_RS02050 0.52 +0.0 458,108 - RR42_RS02050 0.52 +0.0 458,108 - RR42_RS02050 0.52 -0.5
Or see this region's nucleotide sequence