Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01915 and RR42_RS01920 overlap by 4 nucleotides RR42_RS01920 and RR42_RS01925 are separated by 148 nucleotides RR42_RS01925 and RR42_RS01930 are separated by 99 nucleotides
RR42_RS01915: RR42_RS01915 - cytochrome C oxidase subunit I, at 429,622 to 430,725
_RS01915
RR42_RS01920: RR42_RS01920 - protoheme IX farnesyltransferase, at 430,722 to 431,657
_RS01920
RR42_RS01925: RR42_RS01925 - photosynthetic protein synthase I, at 431,806 to 432,432
_RS01925
RR42_RS01930: RR42_RS01930 - RNA polymerase factor sigma-32, at 432,532 to 433,461
_RS01930
Position (kb) 430
431
432 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 429.818 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 430.273 kb on - strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.393 kb on + strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.655 kb on - strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.662 kb on - strand at 430.754 kb on + strand at 431.094 kb on + strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.426 kb on + strand, within RR42_RS01920 at 431.466 kb on - strand, within RR42_RS01920 at 431.481 kb on + strand, within RR42_RS01920 at 431.548 kb on - strand, within RR42_RS01920 at 431.592 kb on + strand at 431.592 kb on + strand at 431.592 kb on + strand at 431.631 kb on + strand at 431.631 kb on + strand at 431.632 kb on - strand at 431.632 kb on - strand at 431.738 kb on + strand at 431.739 kb on - strand at 431.739 kb on - strand at 431.739 kb on - strand at 432.046 kb on - strand, within RR42_RS01925 at 432.046 kb on - strand, within RR42_RS01925 at 432.046 kb on - strand, within RR42_RS01925 at 432.165 kb on + strand, within RR42_RS01925 at 432.165 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.187 kb on + strand, within RR42_RS01925 at 432.188 kb on - strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.277 kb on + strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.311 kb on - strand, within RR42_RS01925 at 432.337 kb on + strand, within RR42_RS01925 at 432.337 kb on + strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.338 kb on - strand, within RR42_RS01925 at 432.477 kb on + strand at 432.511 kb on + strand at 432.512 kb on - strand at 432.526 kb on + strand at 432.526 kb on + strand at 432.526 kb on + strand at 432.527 kb on - strand at 432.527 kb on - strand at 432.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 429,818 - RR42_RS01915 0.18 -1.3 429,929 - RR42_RS01915 0.28 -1.3 429,929 - RR42_RS01915 0.28 -0.1 430,273 - RR42_RS01915 0.59 +0.2 430,276 + RR42_RS01915 0.59 -0.2 430,276 + RR42_RS01915 0.59 -1.8 430,393 + RR42_RS01915 0.70 -0.3 430,394 - RR42_RS01915 0.70 -0.6 430,394 - RR42_RS01915 0.70 -1.2 430,394 - RR42_RS01915 0.70 -1.3 430,655 - +0.0 430,659 + +0.2 430,659 + +0.5 430,659 + +0.2 430,659 + +0.2 430,659 + +0.8 430,661 + -0.3 430,661 + +0.1 430,661 + +0.7 430,662 - +1.2 430,754 + -1.6 431,094 + RR42_RS01920 0.40 -2.0 431,155 - RR42_RS01920 0.46 +1.2 431,155 - RR42_RS01920 0.46 -2.8 431,426 + RR42_RS01920 0.75 -0.7 431,466 - RR42_RS01920 0.79 -1.8 431,481 + RR42_RS01920 0.81 -0.8 431,548 - RR42_RS01920 0.88 -0.3 431,592 + -2.2 431,592 + -0.9 431,592 + -1.7 431,631 + +0.7 431,631 + -1.1 431,632 - +0.4 431,632 - -1.3 431,738 + +0.5 431,739 - +0.8 431,739 - +0.2 431,739 - +0.1 432,046 - RR42_RS01925 0.38 -1.2 432,046 - RR42_RS01925 0.38 -1.3 432,046 - RR42_RS01925 0.38 -2.6 432,165 + RR42_RS01925 0.57 +1.7 432,165 + RR42_RS01925 0.57 -0.8 432,187 + RR42_RS01925 0.61 -2.5 432,187 + RR42_RS01925 0.61 -2.1 432,187 + RR42_RS01925 0.61 -1.2 432,187 + RR42_RS01925 0.61 -4.3 432,188 - RR42_RS01925 0.61 -1.8 432,277 + RR42_RS01925 0.75 -1.5 432,277 + RR42_RS01925 0.75 +1.2 432,277 + RR42_RS01925 0.75 -3.0 432,311 - RR42_RS01925 0.81 -1.3 432,311 - RR42_RS01925 0.81 -2.9 432,311 - RR42_RS01925 0.81 +0.5 432,337 + RR42_RS01925 0.85 -0.9 432,337 + RR42_RS01925 0.85 -1.5 432,338 - RR42_RS01925 0.85 -2.1 432,338 - RR42_RS01925 0.85 -1.3 432,338 - RR42_RS01925 0.85 -1.6 432,338 - RR42_RS01925 0.85 +0.2 432,477 + +0.7 432,511 + -0.4 432,512 - +0.3 432,526 + +0.6 432,526 + -0.2 432,526 + +0.5 432,527 - +0.8 432,527 - +0.3 432,527 - +0.9
Or see this region's nucleotide sequence