Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01905 and RR42_RS01910 are separated by 109 nucleotides RR42_RS01910 and RR42_RS01915 are separated by 7 nucleotides RR42_RS01915 and RR42_RS01920 overlap by 4 nucleotides
RR42_RS01905: RR42_RS01905 - cytochrome C oxidase subunit II, at 428,087 to 428,848
_RS01905
RR42_RS01910: RR42_RS01910 - cytochrome C oxidase subunit I, at 428,958 to 429,614
_RS01910
RR42_RS01915: RR42_RS01915 - cytochrome C oxidase subunit I, at 429,622 to 430,725
_RS01915
RR42_RS01920: RR42_RS01920 - protoheme IX farnesyltransferase, at 430,722 to 431,657
_RS01920
Position (kb) 429
430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 428.640 kb on - strand, within RR42_RS01905 at 428.640 kb on - strand, within RR42_RS01905 at 428.753 kb on + strand, within RR42_RS01905 at 428.754 kb on - strand, within RR42_RS01905 at 428.760 kb on - strand, within RR42_RS01905 at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.957 kb on + strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 428.958 kb on - strand at 429.096 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.105 kb on + strand, within RR42_RS01910 at 429.106 kb on - strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.108 kb on + strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.109 kb on - strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.141 kb on + strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.142 kb on - strand, within RR42_RS01910 at 429.154 kb on - strand, within RR42_RS01910 at 429.403 kb on - strand, within RR42_RS01910 at 429.597 kb on + strand at 429.598 kb on - strand at 429.676 kb on + strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.677 kb on - strand at 429.818 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 429.929 kb on - strand, within RR42_RS01915 at 430.273 kb on - strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.276 kb on + strand, within RR42_RS01915 at 430.393 kb on + strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.394 kb on - strand, within RR42_RS01915 at 430.655 kb on - strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.659 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.661 kb on + strand at 430.662 kb on - strand at 430.754 kb on + strand at 431.094 kb on + strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.155 kb on - strand, within RR42_RS01920 at 431.426 kb on + strand, within RR42_RS01920 at 431.466 kb on - strand, within RR42_RS01920 at 431.481 kb on + strand, within RR42_RS01920 at 431.548 kb on - strand, within RR42_RS01920 at 431.592 kb on + strand at 431.592 kb on + strand at 431.592 kb on + strand at 431.631 kb on + strand at 431.631 kb on + strand at 431.632 kb on - strand at 431.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 428,640 - RR42_RS01905 0.73 -0.1 428,640 - RR42_RS01905 0.73 -0.3 428,753 + RR42_RS01905 0.87 -0.5 428,754 - RR42_RS01905 0.88 +0.2 428,760 - RR42_RS01905 0.88 -2.1 428,957 + +0.1 428,957 + +0.0 428,957 + -0.8 428,957 + +0.1 428,958 - -2.1 428,958 - -0.9 428,958 - +0.0 428,958 - +1.2 429,096 + RR42_RS01910 0.21 -0.4 429,105 + RR42_RS01910 0.22 +0.9 429,105 + RR42_RS01910 0.22 -1.1 429,105 + RR42_RS01910 0.22 -0.3 429,106 - RR42_RS01910 0.23 -0.6 429,108 + RR42_RS01910 0.23 -0.1 429,108 + RR42_RS01910 0.23 +1.2 429,108 + RR42_RS01910 0.23 -0.9 429,109 - RR42_RS01910 0.23 -0.7 429,109 - RR42_RS01910 0.23 +0.5 429,109 - RR42_RS01910 0.23 -1.3 429,141 + RR42_RS01910 0.28 -1.5 429,141 + RR42_RS01910 0.28 -0.1 429,142 - RR42_RS01910 0.28 +0.2 429,142 - RR42_RS01910 0.28 -0.1 429,154 - RR42_RS01910 0.30 +0.7 429,403 - RR42_RS01910 0.68 -0.8 429,597 + -1.1 429,598 - -1.4 429,676 + -1.4 429,677 - -1.3 429,677 - -1.3 429,677 - -2.4 429,677 - -0.1 429,677 - +0.7 429,677 - -1.1 429,818 - RR42_RS01915 0.18 -1.3 429,929 - RR42_RS01915 0.28 -1.3 429,929 - RR42_RS01915 0.28 -0.1 430,273 - RR42_RS01915 0.59 +0.2 430,276 + RR42_RS01915 0.59 -0.2 430,276 + RR42_RS01915 0.59 -1.8 430,393 + RR42_RS01915 0.70 -0.3 430,394 - RR42_RS01915 0.70 -0.6 430,394 - RR42_RS01915 0.70 -1.2 430,394 - RR42_RS01915 0.70 -1.3 430,655 - +0.0 430,659 + +0.2 430,659 + +0.5 430,659 + +0.2 430,659 + +0.2 430,659 + +0.8 430,661 + -0.3 430,661 + +0.1 430,661 + +0.7 430,662 - +1.2 430,754 + -1.6 431,094 + RR42_RS01920 0.40 -2.0 431,155 - RR42_RS01920 0.46 +1.2 431,155 - RR42_RS01920 0.46 -2.8 431,426 + RR42_RS01920 0.75 -0.7 431,466 - RR42_RS01920 0.79 -1.8 431,481 + RR42_RS01920 0.81 -0.8 431,548 - RR42_RS01920 0.88 -0.3 431,592 + -2.2 431,592 + -0.9 431,592 + -1.7 431,631 + +0.7 431,631 + -1.1 431,632 - +0.4 431,632 - -1.3
Or see this region's nucleotide sequence