Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01780 and RR42_RS01785 are separated by 52 nucleotides RR42_RS01785 and RR42_RS01790 overlap by 32 nucleotides RR42_RS01790 and RR42_RS01795 are separated by 139 nucleotides RR42_RS01795 and RR42_RS01800 are separated by 643 nucleotides
RR42_RS01780: RR42_RS01780 - glutathione synthetase, at 405,076 to 406,029
_RS01780
RR42_RS01785: RR42_RS01785 - PTS fructose transporter subunit IIA, at 406,082 to 406,537
_RS01785
RR42_RS01790: RR42_RS01790 - phosphocarrier protein HPr, at 406,506 to 406,775
_RS01790
RR42_RS01795: RR42_RS01795 - phosphoenolpyruvate-protein phosphotransferase, at 406,915 to 408,690
_RS01795
RR42_RS01800: RR42_RS01800 - bacterioferritin, at 409,334 to 409,813
_RS01800
Position (kb) 406
407
408
409 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 406.010 kb on + strand at 406.011 kb on - strand at 406.050 kb on + strand at 406.051 kb on - strand at 406.051 kb on - strand at 406.051 kb on - strand at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.459 kb on - strand, within RR42_RS01785 at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.505 kb on + strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.506 kb on - strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.528 kb on + strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.535 kb on + strand at 406.535 kb on + strand at 406.535 kb on + strand at 406.536 kb on - strand at 406.536 kb on - strand at 406.773 kb on + strand at 406.773 kb on + strand at 406.774 kb on - strand at 406.774 kb on - strand at 406.774 kb on - strand at 406.786 kb on - strand at 406.912 kb on + strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 406.913 kb on - strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.008 kb on + strand at 407.009 kb on - strand at 407.009 kb on - strand at 407.009 kb on - strand at 407.089 kb on - strand at 407.089 kb on - strand at 407.215 kb on + strand, within RR42_RS01795 at 407.215 kb on + strand, within RR42_RS01795 at 407.215 kb on + strand, within RR42_RS01795 at 407.216 kb on - strand, within RR42_RS01795 at 407.287 kb on + strand, within RR42_RS01795 at 407.287 kb on + strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.288 kb on - strand, within RR42_RS01795 at 407.360 kb on + strand, within RR42_RS01795 at 407.360 kb on + strand, within RR42_RS01795 at 407.523 kb on + strand, within RR42_RS01795 at 407.672 kb on - strand, within RR42_RS01795 at 407.672 kb on - strand, within RR42_RS01795 at 407.672 kb on - strand, within RR42_RS01795 at 407.672 kb on - strand, within RR42_RS01795 at 407.730 kb on + strand, within RR42_RS01795 at 407.730 kb on + strand, within RR42_RS01795 at 407.731 kb on - strand, within RR42_RS01795 at 407.855 kb on + strand, within RR42_RS01795 at 407.855 kb on + strand, within RR42_RS01795 at 407.855 kb on + strand, within RR42_RS01795 at 407.855 kb on + strand, within RR42_RS01795 at 407.856 kb on - strand, within RR42_RS01795 at 407.856 kb on - strand, within RR42_RS01795 at 407.856 kb on - strand, within RR42_RS01795 at 407.908 kb on + strand, within RR42_RS01795 at 408.358 kb on + strand, within RR42_RS01795 at 408.358 kb on + strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.359 kb on - strand, within RR42_RS01795 at 408.403 kb on + strand, within RR42_RS01795 at 408.403 kb on + strand, within RR42_RS01795 at 408.404 kb on - strand, within RR42_RS01795 at 408.404 kb on - strand, within RR42_RS01795 at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.649 kb on + strand at 408.650 kb on - strand at 408.650 kb on - strand at 408.650 kb on - strand at 408.716 kb on - strand at 408.735 kb on + strand at 408.735 kb on + strand at 408.735 kb on + strand at 408.736 kb on - strand at 408.739 kb on + strand at 408.799 kb on - strand at 408.820 kb on + strand at 408.820 kb on + strand at 408.821 kb on - strand at 408.821 kb on - strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.876 kb on + strand at 408.877 kb on - strand at 408.928 kb on + strand at 408.929 kb on - strand at 408.983 kb on + strand at 408.983 kb on + strand at 408.984 kb on - strand at 408.984 kb on - strand at 409.019 kb on + strand at 409.019 kb on + strand at 409.406 kb on + strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.407 kb on - strand, within RR42_RS01800 at 409.427 kb on + strand, within RR42_RS01800 at 409.428 kb on - strand, within RR42_RS01800 at 409.428 kb on - strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.466 kb on + strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.467 kb on - strand, within RR42_RS01800 at 409.648 kb on + strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.649 kb on - strand, within RR42_RS01800 at 409.652 kb on + strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.653 kb on - strand, within RR42_RS01800 at 409.674 kb on - strand, within RR42_RS01800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 406,010 + -1.0 406,011 - -0.6 406,050 + -0.7 406,051 - -0.7 406,051 - +0.7 406,051 - +0.8 406,459 - RR42_RS01785 0.83 -0.4 406,459 - RR42_RS01785 0.83 -1.8 406,459 - RR42_RS01785 0.83 -1.9 406,459 - RR42_RS01785 0.83 -2.1 406,505 + -0.6 406,505 + -0.7 406,505 + +0.8 406,505 + -0.7 406,505 + -0.9 406,506 - +0.6 406,506 - -1.8 406,506 - -1.2 406,506 - +0.4 406,506 - -1.2 406,506 - -1.7 406,506 - -1.6 406,506 - -0.8 406,506 - -1.2 406,506 - -0.1 406,506 - -0.9 406,506 - +0.4 406,506 - -1.6 406,506 - +0.3 406,506 - -2.6 406,528 + -1.3 406,528 + -1.1 406,528 + -1.8 406,528 + -1.2 406,528 + -0.7 406,529 - -0.1 406,529 - -0.2 406,529 - -1.3 406,529 - -0.3 406,529 - -2.0 406,529 - -0.8 406,529 - -0.9 406,529 - -2.7 406,529 - -0.6 406,529 - -2.7 406,529 - +0.7 406,529 - -1.4 406,535 + -1.1 406,535 + -0.3 406,535 + -1.6 406,536 - +0.0 406,536 - -0.8 406,773 + +0.1 406,773 + -0.5 406,774 - -1.3 406,774 - -1.5 406,774 - -1.1 406,786 - -1.3 406,912 + +1.3 406,913 - -0.6 406,913 - -0.3 406,913 - -1.0 406,913 - -1.3 406,913 - +0.1 406,913 - +0.1 406,913 - -1.2 406,913 - -0.3 407,008 + -2.6 407,008 + -0.8 407,008 + -3.3 407,008 + -0.8 407,009 - -0.6 407,009 - -1.2 407,009 - -0.4 407,089 - -4.4 407,089 - -1.1 407,215 + RR42_RS01795 0.17 -1.2 407,215 + RR42_RS01795 0.17 -0.1 407,215 + RR42_RS01795 0.17 -1.7 407,216 - RR42_RS01795 0.17 -1.7 407,287 + RR42_RS01795 0.21 -1.3 407,287 + RR42_RS01795 0.21 -0.2 407,288 - RR42_RS01795 0.21 -0.9 407,288 - RR42_RS01795 0.21 -1.0 407,288 - RR42_RS01795 0.21 -0.5 407,288 - RR42_RS01795 0.21 -0.3 407,288 - RR42_RS01795 0.21 -0.6 407,288 - RR42_RS01795 0.21 -0.3 407,288 - RR42_RS01795 0.21 +0.3 407,360 + RR42_RS01795 0.25 -1.0 407,360 + RR42_RS01795 0.25 -1.6 407,523 + RR42_RS01795 0.34 -0.8 407,672 - RR42_RS01795 0.43 -1.4 407,672 - RR42_RS01795 0.43 +2.3 407,672 - RR42_RS01795 0.43 -0.4 407,672 - RR42_RS01795 0.43 -1.0 407,730 + RR42_RS01795 0.46 -0.6 407,730 + RR42_RS01795 0.46 -1.3 407,731 - RR42_RS01795 0.46 -1.8 407,855 + RR42_RS01795 0.53 -1.3 407,855 + RR42_RS01795 0.53 -2.0 407,855 + RR42_RS01795 0.53 +0.0 407,855 + RR42_RS01795 0.53 -1.3 407,856 - RR42_RS01795 0.53 +0.3 407,856 - RR42_RS01795 0.53 -0.1 407,856 - RR42_RS01795 0.53 +0.3 407,908 + RR42_RS01795 0.56 -2.0 408,358 + RR42_RS01795 0.81 -1.4 408,358 + RR42_RS01795 0.81 -0.3 408,359 - RR42_RS01795 0.81 -2.0 408,359 - RR42_RS01795 0.81 -1.3 408,359 - RR42_RS01795 0.81 -1.6 408,403 + RR42_RS01795 0.84 -1.1 408,403 + RR42_RS01795 0.84 -0.2 408,404 - RR42_RS01795 0.84 -1.1 408,404 - RR42_RS01795 0.84 +0.3 408,649 + -1.0 408,649 + -2.1 408,649 + +0.3 408,649 + -2.4 408,649 + +1.8 408,650 - -1.2 408,650 - +0.7 408,650 - -0.6 408,716 - +3.3 408,735 + -0.3 408,735 + -0.1 408,735 + -0.3 408,736 - -1.1 408,739 + -0.7 408,799 - -0.9 408,820 + -0.6 408,820 + -0.3 408,821 - -0.2 408,821 - +0.5 408,876 + -0.2 408,876 + +0.9 408,876 + -0.6 408,876 + -0.3 408,876 + +0.3 408,877 - -1.6 408,928 + +0.1 408,929 - -1.4 408,983 + -0.6 408,983 + -1.3 408,984 - -2.1 408,984 - +0.7 409,019 + -1.1 409,019 + +0.5 409,406 + RR42_RS01800 0.15 +0.1 409,407 - RR42_RS01800 0.15 -0.4 409,407 - RR42_RS01800 0.15 +0.4 409,407 - RR42_RS01800 0.15 +0.2 409,427 + RR42_RS01800 0.19 -0.3 409,428 - RR42_RS01800 0.20 +0.3 409,428 - RR42_RS01800 0.20 +1.1 409,466 + RR42_RS01800 0.28 +0.5 409,466 + RR42_RS01800 0.28 -1.2 409,466 + RR42_RS01800 0.28 +1.8 409,467 - RR42_RS01800 0.28 +1.1 409,467 - RR42_RS01800 0.28 -1.1 409,467 - RR42_RS01800 0.28 +0.1 409,648 + RR42_RS01800 0.65 +3.6 409,649 - RR42_RS01800 0.66 +0.1 409,649 - RR42_RS01800 0.66 +0.4 409,649 - RR42_RS01800 0.66 +1.3 409,649 - RR42_RS01800 0.66 +0.1 409,652 + RR42_RS01800 0.66 +0.3 409,653 - RR42_RS01800 0.66 -0.4 409,653 - RR42_RS01800 0.66 -0.6 409,653 - RR42_RS01800 0.66 -0.6 409,674 - RR42_RS01800 0.71 +1.4
Or see this region's nucleotide sequence