Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01770 and RR42_RS01775 are separated by 458 nucleotides RR42_RS01775 and RR42_RS01780 are separated by 29 nucleotides
RR42_RS01770: RR42_RS01770 - hypothetical protein, at 402,483 to 403,292
_RS01770
RR42_RS01775: RR42_RS01775 - glutamate--cysteine ligase, at 403,751 to 405,046
_RS01775
RR42_RS01780: RR42_RS01780 - glutathione synthetase, at 405,076 to 406,029
_RS01780
Position (kb) 403
404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 403.323 kb on + strand at 403.323 kb on + strand at 403.323 kb on + strand at 403.323 kb on + strand at 403.323 kb on + strand at 403.324 kb on + strand at 403.454 kb on + strand at 403.454 kb on + strand at 403.454 kb on + strand at 403.454 kb on + strand at 403.454 kb on + strand at 403.454 kb on + strand at 403.455 kb on - strand at 403.455 kb on - strand at 403.455 kb on - strand at 403.455 kb on - strand at 403.455 kb on - strand at 403.595 kb on + strand at 403.595 kb on + strand at 403.595 kb on + strand at 403.596 kb on - strand at 403.611 kb on - strand at 403.632 kb on - strand at 403.632 kb on - strand at 403.667 kb on + strand at 403.667 kb on + strand at 403.668 kb on - strand at 403.673 kb on + strand at 403.673 kb on + strand at 403.673 kb on + strand at 403.673 kb on + strand at 403.674 kb on - strand at 403.674 kb on - strand at 403.674 kb on - strand at 403.674 kb on - strand at 403.741 kb on + strand at 403.741 kb on + strand at 403.750 kb on + strand at 403.750 kb on + strand at 403.751 kb on - strand at 403.864 kb on + strand at 403.864 kb on + strand at 403.864 kb on + strand at 403.864 kb on + strand at 403.864 kb on + strand at 403.865 kb on - strand at 404.337 kb on - strand, within RR42_RS01775 at 404.337 kb on - strand, within RR42_RS01775 at 404.562 kb on - strand, within RR42_RS01775 at 404.591 kb on + strand, within RR42_RS01775 at 404.591 kb on + strand, within RR42_RS01775 at 404.592 kb on - strand, within RR42_RS01775 at 404.622 kb on - strand, within RR42_RS01775 at 404.622 kb on - strand, within RR42_RS01775 at 404.795 kb on + strand, within RR42_RS01775 at 404.795 kb on + strand, within RR42_RS01775 at 404.796 kb on - strand, within RR42_RS01775 at 404.829 kb on - strand, within RR42_RS01775 at 404.964 kb on - strand at 404.965 kb on + strand at 404.965 kb on + strand at 404.966 kb on - strand at 404.966 kb on - strand at 404.966 kb on - strand at 404.969 kb on + strand at 404.970 kb on - strand at 404.970 kb on - strand at 404.970 kb on - strand at 405.122 kb on - strand at 405.136 kb on + strand at 405.136 kb on + strand at 405.355 kb on + strand, within RR42_RS01780 at 405.356 kb on - strand, within RR42_RS01780 at 405.356 kb on - strand, within RR42_RS01780 at 405.472 kb on + strand, within RR42_RS01780 at 405.472 kb on + strand, within RR42_RS01780 at 405.473 kb on - strand, within RR42_RS01780 at 405.511 kb on - strand, within RR42_RS01780 at 405.511 kb on - strand, within RR42_RS01780 at 405.670 kb on + strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.671 kb on - strand, within RR42_RS01780 at 405.672 kb on + strand, within RR42_RS01780 at 405.673 kb on - strand, within RR42_RS01780 at 405.673 kb on - strand, within RR42_RS01780 at 405.733 kb on + strand, within RR42_RS01780 at 405.748 kb on - strand, within RR42_RS01780 at 405.904 kb on + strand, within RR42_RS01780 at 405.905 kb on - strand, within RR42_RS01780 at 406.010 kb on + strand at 406.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 403,323 + -0.0 403,323 + -0.1 403,323 + +0.7 403,323 + +0.4 403,323 + -0.1 403,324 + +0.2 403,454 + -1.7 403,454 + -0.8 403,454 + +0.3 403,454 + +0.3 403,454 + +0.8 403,454 + +0.7 403,455 - -0.2 403,455 - +0.5 403,455 - -0.2 403,455 - +0.3 403,455 - +0.5 403,595 + +0.4 403,595 + +0.4 403,595 + +0.1 403,596 - +0.2 403,611 - -0.7 403,632 - -0.2 403,632 - -1.1 403,667 + +0.2 403,667 + +0.1 403,668 - -0.2 403,673 + -0.2 403,673 + -3.3 403,673 + +0.2 403,673 + +0.6 403,674 - +0.7 403,674 - +1.0 403,674 - -0.8 403,674 - -1.2 403,741 + +0.8 403,741 + +0.3 403,750 + -0.8 403,750 + -0.1 403,751 - -1.0 403,864 + -2.3 403,864 + -0.4 403,864 + -1.2 403,864 + -0.5 403,864 + +0.0 403,865 - -0.3 404,337 - RR42_RS01775 0.45 -0.6 404,337 - RR42_RS01775 0.45 -0.6 404,562 - RR42_RS01775 0.63 -1.2 404,591 + RR42_RS01775 0.65 -2.4 404,591 + RR42_RS01775 0.65 -1.2 404,592 - RR42_RS01775 0.65 -1.2 404,622 - RR42_RS01775 0.67 -0.2 404,622 - RR42_RS01775 0.67 -1.8 404,795 + RR42_RS01775 0.81 -2.1 404,795 + RR42_RS01775 0.81 -0.7 404,796 - RR42_RS01775 0.81 -0.7 404,829 - RR42_RS01775 0.83 -2.2 404,964 - -0.7 404,965 + -2.1 404,965 + -0.9 404,966 - -0.7 404,966 - -1.5 404,966 - -0.7 404,969 + -1.2 404,970 - -2.3 404,970 - -1.8 404,970 - -1.6 405,122 - +1.0 405,136 + +1.6 405,136 + -2.3 405,355 + RR42_RS01780 0.29 -1.5 405,356 - RR42_RS01780 0.29 -1.1 405,356 - RR42_RS01780 0.29 +0.3 405,472 + RR42_RS01780 0.42 -1.4 405,472 + RR42_RS01780 0.42 +1.8 405,473 - RR42_RS01780 0.42 -0.7 405,511 - RR42_RS01780 0.46 +0.6 405,511 - RR42_RS01780 0.46 -0.1 405,670 + RR42_RS01780 0.62 -1.7 405,671 - RR42_RS01780 0.62 +1.3 405,671 - RR42_RS01780 0.62 -0.8 405,671 - RR42_RS01780 0.62 -1.2 405,671 - RR42_RS01780 0.62 +1.3 405,672 + RR42_RS01780 0.62 -2.1 405,673 - RR42_RS01780 0.63 -1.1 405,673 - RR42_RS01780 0.63 -1.7 405,733 + RR42_RS01780 0.69 -1.7 405,748 - RR42_RS01780 0.70 -1.4 405,904 + RR42_RS01780 0.87 -0.6 405,905 - RR42_RS01780 0.87 -0.9 406,010 + -1.0 406,011 - -0.6
Or see this region's nucleotide sequence