Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01625 and RR42_RS01630 overlap by 4 nucleotides RR42_RS01630 and RR42_RS01635 are separated by 24 nucleotides RR42_RS01635 and RR42_RS01640 are separated by 26 nucleotides
RR42_RS01625: RR42_RS01625 - long-chain fatty acid--CoA ligase, at 369,975 to 371,945
_RS01625
RR42_RS01630: RR42_RS01630 - branched-chain amino acid ABC transporter ATP-binding protein, at 371,942 to 372,865
_RS01630
RR42_RS01635: RR42_RS01635 - ABC transporter permease, at 372,890 to 373,804
_RS01635
RR42_RS01640: RR42_RS01640 - ABC transporter permease, at 373,831 to 374,907
_RS01640
Position (kb) 371
372
373 Strain fitness (log2 ratio) -1
0
1
2 at 371.149 kb on - strand, within RR42_RS01625 at 371.149 kb on - strand, within RR42_RS01625 at 371.274 kb on + strand, within RR42_RS01625 at 371.275 kb on - strand, within RR42_RS01625 at 371.277 kb on + strand, within RR42_RS01625 at 371.277 kb on + strand, within RR42_RS01625 at 371.322 kb on + strand, within RR42_RS01625 at 371.433 kb on + strand, within RR42_RS01625 at 371.464 kb on - strand, within RR42_RS01625 at 371.465 kb on + strand, within RR42_RS01625 at 371.571 kb on + strand, within RR42_RS01625 at 371.572 kb on - strand, within RR42_RS01625 at 371.580 kb on + strand, within RR42_RS01625 at 371.580 kb on + strand, within RR42_RS01625 at 371.766 kb on + strand at 371.767 kb on - strand at 371.767 kb on - strand at 371.767 kb on - strand at 371.768 kb on + strand at 371.768 kb on + strand at 371.805 kb on + strand at 371.900 kb on - strand at 371.900 kb on - strand at 371.900 kb on - strand at 372.163 kb on + strand, within RR42_RS01630 at 372.258 kb on - strand, within RR42_RS01630 at 372.924 kb on + strand at 372.974 kb on + strand at 372.975 kb on - strand at 373.019 kb on + strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.020 kb on - strand, within RR42_RS01635 at 373.460 kb on - strand, within RR42_RS01635 at 373.460 kb on - strand, within RR42_RS01635 at 373.597 kb on + strand, within RR42_RS01635 at 373.664 kb on + strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.665 kb on - strand, within RR42_RS01635 at 373.731 kb on - strand at 373.731 kb on - strand at 373.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 371,149 - RR42_RS01625 0.60 +2.1 371,149 - RR42_RS01625 0.60 +0.5 371,274 + RR42_RS01625 0.66 -0.6 371,275 - RR42_RS01625 0.66 -0.3 371,277 + RR42_RS01625 0.66 +0.3 371,277 + RR42_RS01625 0.66 +0.3 371,322 + RR42_RS01625 0.68 +0.5 371,433 + RR42_RS01625 0.74 +0.6 371,464 - RR42_RS01625 0.76 +0.5 371,465 + RR42_RS01625 0.76 +0.7 371,571 + RR42_RS01625 0.81 -0.5 371,572 - RR42_RS01625 0.81 -0.5 371,580 + RR42_RS01625 0.81 +2.3 371,580 + RR42_RS01625 0.81 -0.0 371,766 + -0.7 371,767 - -0.7 371,767 - -1.3 371,767 - +0.3 371,768 + -0.0 371,768 + +0.5 371,805 + +0.3 371,900 - +1.7 371,900 - +0.7 371,900 - -1.1 372,163 + RR42_RS01630 0.24 +1.5 372,258 - RR42_RS01630 0.34 +1.9 372,924 + +1.4 372,974 + +0.3 372,975 - +0.7 373,019 + RR42_RS01635 0.14 +1.6 373,020 - RR42_RS01635 0.14 -0.5 373,020 - RR42_RS01635 0.14 -0.5 373,020 - RR42_RS01635 0.14 -0.8 373,020 - RR42_RS01635 0.14 +0.2 373,460 - RR42_RS01635 0.62 -1.2 373,460 - RR42_RS01635 0.62 -0.3 373,597 + RR42_RS01635 0.77 -0.9 373,664 + RR42_RS01635 0.85 -0.1 373,665 - RR42_RS01635 0.85 +0.5 373,665 - RR42_RS01635 0.85 +2.1 373,665 - RR42_RS01635 0.85 -1.0 373,731 - +0.7 373,731 - -0.3 373,731 - +0.2
Or see this region's nucleotide sequence