Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01410 and RR42_RS01415 are separated by 200 nucleotides RR42_RS01415 and RR42_RS01420 are separated by 8 nucleotides RR42_RS01420 and RR42_RS01425 are separated by 727 nucleotides RR42_RS01425 and RR42_RS01430 are separated by 38 nucleotides
RR42_RS01410: RR42_RS01410 - NAD-dependent dehydratase, at 318,260 to 319,285
_RS01410
RR42_RS01415: RR42_RS01415 - glutathione S-transferase, at 319,486 to 320,175
_RS01415
RR42_RS01420: RR42_RS01420 - 2', 3'-cyclic nucleotide 2'-phosphodiesterase, at 320,184 to 321,419
_RS01420
RR42_RS01425: RR42_RS01425 - hypothetical protein, at 322,147 to 322,341
_RS01425
RR42_RS01430: RR42_RS01430 - hypothetical protein, at 322,380 to 323,579
_RS01430
Position (kb) 320
321
322 Strain fitness (log2 ratio) -2
-1
0
1
2 at 319.351 kb on - strand at 319.351 kb on - strand at 319.382 kb on + strand at 319.382 kb on + strand at 319.383 kb on - strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.643 kb on + strand, within RR42_RS01415 at 319.643 kb on + strand, within RR42_RS01415 at 319.644 kb on - strand, within RR42_RS01415 at 319.699 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.006 kb on - strand, within RR42_RS01415 at 320.038 kb on + strand, within RR42_RS01415 at 320.193 kb on + strand at 320.193 kb on + strand at 320.859 kb on + strand, within RR42_RS01420 at 321.005 kb on + strand, within RR42_RS01420 at 321.463 kb on - strand at 322.039 kb on + strand at 322.087 kb on + strand at 322.088 kb on - strand at 322.131 kb on - strand at 322.138 kb on + strand at 322.138 kb on + strand at 322.355 kb on + strand at 322.355 kb on + strand at 322.356 kb on - strand at 322.381 kb on + strand at 322.381 kb on + strand at 322.381 kb on + strand at 322.382 kb on - strand at 322.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 319,351 - -0.6 319,351 - +1.5 319,382 + +2.0 319,382 + +0.8 319,383 - -0.7 319,513 + -0.7 319,513 + -0.2 319,513 + -0.7 319,643 + RR42_RS01415 0.23 -1.0 319,643 + RR42_RS01415 0.23 +0.1 319,644 - RR42_RS01415 0.23 -0.1 319,699 + RR42_RS01415 0.31 +0.1 320,005 + RR42_RS01415 0.75 -0.5 320,005 + RR42_RS01415 0.75 +0.1 320,005 + RR42_RS01415 0.75 -0.6 320,005 + RR42_RS01415 0.75 +0.0 320,006 - RR42_RS01415 0.75 +0.8 320,038 + RR42_RS01415 0.80 -0.1 320,193 + +0.7 320,193 + +1.1 320,859 + RR42_RS01420 0.55 -0.9 321,005 + RR42_RS01420 0.66 +0.3 321,463 - -0.2 322,039 + -0.2 322,087 + -2.6 322,088 - -0.0 322,131 - -1.0 322,138 + -0.6 322,138 + +1.4 322,355 + +0.6 322,355 + +0.6 322,356 - -0.7 322,381 + -0.8 322,381 + -0.2 322,381 + -0.0 322,382 - -0.2 322,384 - -0.6
Or see this region's nucleotide sequence